Recent advances in capillary electrophoresis and capillary electrochromatography of peptides

Kasicka,

doi:10.1002/elps.200305660

Cited by 106 publications

(67 citation statements)

References 283 publications

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“…In particular, flow separation methods, such as chromatography, 1,2 electrophoresis, [3][4][5][6][7] and field flow fractionation (FFF), [8][9][10] have been extensively used in various fundamental and practical fields, and have facilitated the advancements of modern science. These methods cover separation of almost all classes of materials.…”

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confidence: 99%

Solute Distribution Coupled with Laminar Flow in Wide-Bore Capillaries: What Can Be Separated without Chemical or Physical Fields?

et al. 2005

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A number of researches have been devoted to the developments of novel separation principles and methodology, because material separation is one of the most important fundamentals in scientific and technological disciplines. In particular, flow separation methods, such as chromatography, 1,2 electrophoresis, [3][4][5][6][7] and field flow fractionation (FFF), [8][9][10] have been extensively used in various fundamental and practical fields, and have facilitated the advancements of modern science. These methods cover separation of almost all classes of materials. A number of dissolved molecules, from simple ions to macromolecules, can be separated by chromatography or electrophoresis, FFF and electrophoresis being capable of resolving even particles. The dimensions of solutes to be separated by these methods thus range from 10 -10 m to 10 -5 m. This situation may suggest that separation science and technology have been completely matured. However, studying new separation principles is still important, because they can allow the separation based on material properties that have not been utilized for conventional methods, provide higher performance and more efficient separation than existing means, and facilitate the understanding of materials through separation processes.According to chromatographic theories, the intrinsic separation performance of liquid chromatography is restricted in comparison with that of gas chromatography because of much lower solute diffusion in liquid phases. 11However, a liquid flow obviously provides versatile ways for material separation because liquids have higher density and dissolution power than gases, and thus has made possible application to various types of solutes with wide ranges in molecular weights and sizes. In the usual flow separation methods, appropriate chemical (chromatography) or physical (FFF) separation fields are created, which retain solutes to different extents and separate them. A variety of stationary phases for chromatography 2,[12][13][14] and physical forces for FFF [15][16][17][18][19] have been exploited, and have provided different capabilities and selectivity from those performed by conventional separation. In contrast, a separation method that requires neither chemical interactions nor special external fields should also be useful because of its instrumental simplicity. Hydrodynamic chromatography (HDC) is such a case, which allows separation of particles or macromolecules according to their hydrodynamic sizes just by passing them through a narrow channel. [20][21][22][23][24] Particles with larger diameter for example cannot approach the channel wall, and thus flow through the channel faster than the smaller particles, when the laminar flow profile is maintained therein. Although the applicability of this method was very restricted, recent developments of chip technology have brought about a remarkable progress. 23,24 If a capillary of radius a is used for HDC, the time for the elution of a particle of radius rp is given bywhere t0 is the time required ...

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Solute Distribution Coupled with Laminar Flow in Wide-Bore Capillaries: What Can Be Separated without Chemical or Physical Fields?

et al. 2005

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“…The same group reported another approach to label proteins on-capillary with FQ [114], in which the labelling reaction is performed directly in the capillary by EMMA. The fluorescent derivative formed with conalbumin in the presence of potassium cyanide as coreagent at a temperature of 657C was detected with an LOD of 3610 24 nM, corresponding to an improvement of 7610 6 -fold compared to UV detection. This group also employed EMMA-FQ combined to field-enhanced stacking for a selective label of different components in a protein mixture from six Staphylococcus species [115].…”

Section: -(2-furoyl)quinoline-2-carboxaldehyde (Fq)mentioning

confidence: 99%

“…Specific reviews or books dedicated to CE analysis of peptides [4][5][6][7][8][9][10] and proteins [7][8][9][10][11][12][13][14][15] have been published, outlining that the extremely small id of the capillary has the advantage of requiring small sample volumes but often presents poor mass sensitivity. This is observed particularly with UV absorbance detection due to the short optical path length across the capillary.…”

Section: Introductionmentioning

confidence: 99%

LIF detection of peptides and proteins in CE

2007

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CE- and microchip-based separations coupled with LIF are powerful tools for the separation, detection and determination of biomolecules. CE with certain configurations has the potential to detect a small number of molecules or even a single molecule, thanks to the high spatial coherence of the laser source which permits the excitation of very small sample volumes with high efficiency. This review article discusses the use of LIF detection for the analysis of peptides and proteins in CE. The most common laser sources, basic instrumentation, derivatization modes and set-ups are briefly presented and special attention is paid to the different fluorogenic agents used for pre-, on- and postcapillary derivatization of the functional groups of these compounds. A table summarizing major applications of these derivatization reactions to the analysis of peptides and proteins in CE-LIF and a bibliography with 184 references are provided which covers papers published to the end of 2005.

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“…The increasing use of this coupled technique can be inferred by the high number of reviews published involving the use of CE-MS for proteins and peptides analysis [5][6][7][8][9][10][11][12] as well as for other biomolecules [7,[13][14][15] or more general CE-MS developments and applications [16][17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis‐electrospray‐mass spectrometry in peptide analysis and peptidomics

2008

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In the present work, an exhaustive review of the main developments and applications of CE-MS for peptide analysis is given. This review includes the use of different CE separation modes, MS analyzers, capillary coatings, preconcentration techniques, on-chip applications as well as other different multidimensional strategies for peptide analysis. Key applications are critically discussed and relevant works published from January 2000 to May 2007 are summarized including information concerning the type of sample, CE-MS parameters as well as some figures of merit of the different CE-MS procedures developed for peptide analysis and peptidomics.

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Recent advances in capillary electrophoresis and capillary electrochromatography of peptides

Cited by 106 publications

References 283 publications

Solute Distribution Coupled with Laminar Flow in Wide-Bore Capillaries: What Can Be Separated without Chemical or Physical Fields?

Solute Distribution Coupled with Laminar Flow in Wide-Bore Capillaries: What Can Be Separated without Chemical or Physical Fields?

LIF detection of peptides and proteins in CE

Capillary electrophoresis‐electrospray‐mass spectrometry in peptide analysis and peptidomics

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