Capacitive field-effect biosensor modified with a stacked bilayer of weak polyelectrolyte and plant virus particles as enzyme nanocarriers

Welden, Melanie; Poghossian, Arshak; Vahidpour, Farnoosh; Wendlandt, Tim; Keusgen, Michael; ChristinaWege,; Schöning, Michael J.

doi:10.1016/j.bioelechem.2023.108397

Cited by 10 publications

(19 citation statements)

References 69 publications

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“…The Ta 2 O 5 -gate EISCAPs were used for multiplexed pH measurements, because Ta 2 O 5 belongs to the best pHsensitive materials, exhibiting a nearly-Nernstian pH sensitivity, small drift and a low hysteresis. [1,25,27,49,60] The fabrication process steps of EISCAPs were described previously (see e.g., [7,17] ). Briefly, the Ta 2 O 5 layer (60 nm) was prepared by electron-beam evaporation of a 30 nm tantalum layer and subsequent oxidation under oxygen atmosphere at 520 °C for 60 min.…”

Section: Methodsmentioning

confidence: 99%

“…The fabrication process steps of EISCAPs were described previously (see e.g., [ 7,17 ] ). Briefly, the Ta 2 O 5 layer (60 nm) was prepared by electron‐beam evaporation of a 30 nm tantalum layer and subsequent oxidation under oxygen atmosphere at 520 °C for 60 min.…”

Section: Methodsmentioning

confidence: 99%

“…Similar to other types of field-effect biochemical sensors, EISCAPs are very sensitive to any kind of potential (or charge) changes induced via biochemical reactions at or nearby the gate-insulator/electrolyte interface. Due to the simple structure and easy fabrication, the EISCAP platform has been widely applied for designing a large variety of biochemical sensors capable of concentration measurements of ions [1][2][3] and metabolites, [4][5][6][7][8][9] as well as for the label-free detection of charged biomolecules, [10][11][12][13][14][15] virus particles, [16][17][18] carbon nanotubes, [19] and nanoparticle/molecule hybrids [20][21][22] by their intrinsic charge. Moreover, EISCAPs have been applied for the creation of biomolecular logic gates.…”

Section: Introductionmentioning

confidence: 99%

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Multiplexing System for Automated Characterization of a Capacitive Field‐Effect Sensor Array

Karschuck

Schmidt

Achtsnicht

et al. 2023

Physica Status Solidi (a)

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In comparison to single‐analyte devices, multiplexed systems for a multi‐analyte detection offer a reduced assay time and sample volume, low costs and high throughput. In this work, a multiplexing platform for an automated quasi‐simultaneous characterization of multiple (up to sixteen) capacitive field‐effect sensors by the capacitive‐voltage (C−V) and the constant‐capacitance (ConCap) mode is presented. The sensors were mounted in a newly designed multi‐cell arrangement with one common reference electrode and electrically connected to the impedance analyzer via the base station. A Python script for the automated characterization of the sensors executes the user‐defined measurement protocol. The developed multiplexing system was tested for pH measurements and the label‐free detection of ligand‐stabilized, charged gold nanoparticles.This article is protected by copyright. All rights reserved.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multiplexing System for Automated Characterization of a Capacitive Field‐Effect Sensor Array

Karschuck

Schmidt

Achtsnicht

et al. 2023

Physica Status Solidi (a)

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“…Reproduced with permission. [72] Copyright 2023, Elsevier B.V. dynamics of TMV. Meanwhile, in ELISA, TMV-functionalized impedance sensors responded to concentrations ranging from 12 ng mL À1 to 1.2 μg mL À1 anti-Flag IgG antibodies, resulting in an 18-35% increase in total impedance, respectively.…”

Section: Biosensingmentioning

confidence: 99%

Advances of Structural Design and Biomedical Applications of Tobacco Mosaic Virus Coat Protein

Zhang,

He,

Zeng

et al. 2024

Advanced NanoBiomed Research

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Recognized as the primary RNA virus to be categorized and extensively studied, the tobacco mosaic virus (TMV) is foundational to advancements in virology, molecular biology, and biomaterials. Over the past few decades, the deep comprehension of the TMV coat protein (TMVcp) molecular structure and assembly principles has stimulated a surge in research on TMVcp structural design using genetic engineering and chemical modification techniques. The unique characteristics of TMVcp, including its nanoscale orderly structure, ease of modification, and considerable drug loading capacity, have enabled significant progress in its biomedical applications. This review summarizes the advanced strategies deployed for TMVcp design and multidimensional assembly and underscores the prototypical applications of TMVcp‐based biomaterials in bioimaging, drug delivery, tissue engineering, and biosensing. Ultimately, the future prospects of TMVcp research in structural design and biomedical applications are explored, which encompass artificial intelligence‐guided structural and functional design, the development of stimulus‐responsive biomaterials, and potential clinical translation.

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“…With the spatially well-defined GM spot array and a known number of docking sites exposed on the TMV nanocarriers, it will be feasible to integrate a defined number of enzyme molecules per spot and to combine distinct enzymes on one biosensor platform via GM formulations with different nanoparticle-enzyme conjugates. TMV has already been successfully applied in various sensor layouts [39][40][41][62][63][64][65][66][67][68][69][70] and was shown to stabilize distinct enzymes including penicillinase in label-free electrochemical field-effect biosensors that retained their perfor-mance for at least a year of repetitive use. [41] This points to the future potential of the combination of plant viral bioscaffolds with hydrogel soft biosensors, for example, as sensor domains in 3D cell cultures grown for tissue replacement, in implanted online sensors, or for in situ detection purposes for process monitoring in biotechnological or food fabrication fermenters.…”

Section: Assembly Of the All-soft Biosensormentioning

confidence: 99%

Soft Sub‐Structured Multi‐Material Biosensor Hydrogels with Enzymes Retained by Plant Viral Scaffolds

Grübel,

Wendlandt,

Urban

et al. 2023

Macromolecular Bioscience

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An all‐soft multi‐material combination consisting of a hydrogel based on poly(ethylene glycol) (PEG) coated with spatially defined spots of gelatin methacryloyl (GM) containing selectively addressable viral nanorods is presented, and its basic application as a qualitative biosensor with reporter enzymes displayed on the tobacco mosaic virus (TMV) bioscaffolds within the GM is demonstrated. Biologically inert PEG supports are equipped with GM spots serving as biological matrix for enzymes clustered on TMV particles preventing diffusion out of the gel. For this multi‐material combination, i) the PEG‐based hydrogel surface is modified to achieve a clear boundary between coated and non‐coated regions by introducing either isothiouronium or thiol groups. ii) Cross‐linking of the GM spots is studied to achieve anchoring to the hydrogel surface. iii) The enzymes horseradish peroxidase or penicillinase (Pen) are conjugated to TMV and integrated into the GM matrix. In contrast to free enzymes, enzyme‐decorated TMVs persist in GM spots and show sustained enzyme activity as evidenced by specific color reaction after 7 days of washing, and for Pen after 22 months after dry storage. Therefore, the integration of enzyme‐coupled TMV into hydrogel matrices is a promising and versatile approach to obtaining reusable and analyte‐specific sensor components.

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Capacitive field-effect biosensor modified with a stacked bilayer of weak polyelectrolyte and plant virus particles as enzyme nanocarriers

Cited by 10 publications

References 69 publications

Multiplexing System for Automated Characterization of a Capacitive Field‐Effect Sensor Array

Multiplexing System for Automated Characterization of a Capacitive Field‐Effect Sensor Array

Advances of Structural Design and Biomedical Applications of Tobacco Mosaic Virus Coat Protein

Soft Sub‐Structured Multi‐Material Biosensor Hydrogels with Enzymes Retained by Plant Viral Scaffolds

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