Capacitative calcium entry and proliferation of human osteoblast‐like MG‐63 cells

Labelle, Dominique; Jumarie, Catherine; Moreau, Robert A.

doi:10.1111/j.1365-2184.2007.00477.x

Cited by 28 publications

(36 citation statements)

References 78 publications

(127 reference statements)

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“…The cell population doubling times estimated in this study are presented in Table II. The MG63 cells doubling time growing on the polystyrene was 29.5 ± 3.7 h, and it is similar to results obtained by others [10,11]. The cell population doubling time on the PCL was twice higher than the control (58.3 vs. 29.5 h).…”

Section: Human Osteoblast-like Mg 63 Cells Proliferation On Nanocomposupporting

confidence: 79%

“…For example the cellular redox activity of succinate dehydrogenase might be evaluated by the tetrazolium salt reduction (express as colored end products) in tests: MTT (3-(4,5--dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole reduced to purple formazan in living cells) or XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)--2H-tetrazolium-5-carboxanilide -more sensitive, the formed formazan dye is water soluble, which allows avoiding a final solubilization step). These tests are recommended for measurement of cytotoxicity and proliferation ability [10]. Figure 1 shows the results of comparison of the number of cells growing on the nanocomposites with the number of cells in the control material (dedicated to the conducting cell cultures).…”

Section: Human Osteoblast-like Mg 63 Cells Proliferation On Nanocompomentioning

confidence: 99%

“…Such high cellular survival means that the applied nanocomposites as well as polymer matrix do not exhibit cytotoxic properties. However it is suggested [10] that for certainty, non-cytotoxic property should be additionally confirmed in other tests. In this case the colorimetric assays measuring the enzyme activity that are normally inside cells might be applied.…”

Section: Human Osteoblast-like Mg 63 Cells Proliferation On Nanocompomentioning

confidence: 99%

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The Study of Human Osteoblast-Like MG 63 Cells Proliferation on Resorbable Polymer-Based Nanocomposites Modified with Ceramic and Carbon Nanoparticles

et al. 2012

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Polymer-based nanocomposites containing biocompatible and bioactive nanocomponents seem to be excellent materials that could be used in many biomedical applications. The aim of this study was biological evaluation of resorbable polymer-based nanocomposites (PLA, PCL) and their modifications with ceramic nanoparticles (silica -SiO 2 , montmorylonit -MMT) or carbon nanotubes. The nanocomposites were seeded with the human osteoblast-like MG 63 cells. After 1, 3 and 7 days of incubation, Trypan blue exclusion test was used to determine the viability and number of cells. The cell population density depending on incubation time and cell population doubling time was calculated. The cell proliferation abilities on the all applied nanocomposites and on control material (polystyrene cell culture plate) were also compared. The number of cells growing on the nanocomposite surfaces increased with the incubation time. The cell viability was not decreased for all applied materials during the entire study (97-100%). The ceramic nanoparticles and carbon nanotubes modified the bone cell growth and proliferation rate. Results of this study confirm that all types of the nanocomposites are appropriate to the growing and proliferation of human osteoblast-like cells.

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Section: Human Osteoblast-like Mg 63 Cells Proliferation On Nanocomposupporting

confidence: 79%

Section: Human Osteoblast-like Mg 63 Cells Proliferation On Nanocompomentioning

confidence: 99%

Section: Human Osteoblast-like Mg 63 Cells Proliferation On Nanocompomentioning

confidence: 99%

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The Study of Human Osteoblast-Like MG 63 Cells Proliferation on Resorbable Polymer-Based Nanocomposites Modified with Ceramic and Carbon Nanoparticles

et al. 2012

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“…3B), corresponding to a 42% diminution in comparison to the scramble (p < 0.05). Our results for MSC indicate that the TRPC1 protein also plays an important role in the proliferation of stem cells, in accordance with recent results for breast cancer cells [31] and osteoblasts [32]. It should be noted that the hepatoma cell proliferation depended on the expression of the TRPC6 gene but not on that of the TRPC1 gene [29], indicating the necessity of a detailed study of the localization and functionality of the two proteins in the MSC.…”

Section: Resultssupporting

confidence: 77%

TRPC expression in mesenchymal stem cells

Torossian¹,

Bisson

Vannier

et al. 2010

Cellular and Molecular Biology Letters

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Transient receptor potential canonical (TRPC) channels are key players in calcium homeostasis and various regulatory processes in cell biology. Little is currently known about the TRPC subfamily members in mesenchymal stem cells (MSC), where they could play a role in cell proliferation. We report on the presence of TRPC1, 2, 4 and 6 mRNAs in MSC. Western blot and immunofluorescence staining indicate a membrane and intracellular distribution of TRPC1. Furthermore, the decrease in the level of TRPC1 protein caused by RNA interference is accompanied by the downregulation of cell proliferation. These results indicate that MSC express TRPC1, 2, 4 and 6 mRNA and that TRPC1 may play a role in stem cell proliferation.

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“…First, a significant increase in MTT activity was seen as soon as 9 h after the addition of oxLDL to the incubation media. Under high cell proliferation rate in the presence of serum, MG-63 cells rather showed doubling time of 28 h (22). Therefore completion of cell cycle that would be associated with increased cell number and MTT activity within 9 h is unlikely.…”

Section: Determination Of Hormesis-like Effects Induced By Oxldl and mentioning

confidence: 99%

Characterization of oxidized low-density lipoprotein-induced hormesis-like effects in osteoblastic cells

Hamel¹,

Abed²,

Brissette³

et al. 2008

American Journal of Physiology-Cell Physiology

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Hamel P, Abed E, Brissette L, Moreau R. Characterization of oxidized low-density lipoprotein-induced hormesis-like effects in osteoblastic cells. Am J Physiol Cell Physiol 294: C1021-C1033, 2008. First published February 20, 2008 doi:10.1152/ajpcell.00361.2007.-Epidemiological studies indicate that patients suffering from atherosclerosis are predisposed to develop osteoporosis. Atherogenic determinants such as oxidized low-density lipoprotein (oxLDL) particles have been shown both to stimulate the proliferation and promote apoptosis of bone-forming osteoblasts. Given such opposite responses, we characterized the oxLDL-induced hormesis-like effects in osteoblasts. Biphasic 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reductive activity responses were induced by oxLDL where low concentrations (10 -50 g/ml) increased and high concentrations (from 150 g/ml) reduced the MTT activity. Cell proliferation stimulation by oxLDL partially accounted for the increased MTT activity. No alteration of mitochondria mass was noticed, whereas low concentrations of oxLDL induced mitochondria hyperpolarization and increased the cellular levels of reactive oxygen species (ROS). The oxLDL-induced MTT activity was not related to intracellular ROS levels. OxLDL increased NAD(P)H-associated cellular fluorescence and flavoenzyme inhibitor diphenyleneiodonium reduced basal and oxLDL-induced MTT activity, suggesting an enhancement of NAD(P)H-dependent cellular reduction potential. Low concentrations of oxLDL reduced cellular thiol content and increased metallothionein expression, suggesting the induction of compensatory mechanisms for the maintenance of cell redox state. These concentrations of oxLDL reduced osteoblast alkaline phosphatase activity and cell migration. Our results indicate that oxLDL particles cause hormesis-like response with the stimulation of both proliferation and cellular NAD(P)H-dependent reduction potential by low concentrations, whereas high concentrations lead to reduction of MTT activity associated with the cell death. Given the effects of low concentrations of oxLDL on osteoblast functions, oxLDL may contribute to the impairment of bone remodeling equilibrium.osteoblasts; atherosclerosis; oxysterol ELEVATED LEVELS of serum low-density lipoprotein (LDL) particles are considered as the most important atherogenic risk factor. LDL particles are thought to become atherogenic after undergoing oxidative modifications, and key roles of oxidized LDL (various oxidized species collectively designated oxLDL) in atherosclerosis have been largely reviewed by Steinberg (46). OxLDL and oxysterols have been shown to increase or decrease proliferation and trigger the apoptosis process depending on the cell types related to vasculature alterations, nature of oxLDL and oxysterols, and on the concentrations used (52), thus supporting numerous deleterious effects that sustain the development of atherosclerosis. Also, a number of clinical studies suggest an association between cardiovascular disease and the develo...

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Capacitative calcium entry and proliferation of human osteoblast‐like MG‐63 cells

Abstract: Our results indicate that CCE, likely attributed to the activation of TRPCs, might be the main route for Ca2+ influx involved in osteoblast proliferation.

Cited by 28 publications

References 78 publications

The Study of Human Osteoblast-Like MG 63 Cells Proliferation on Resorbable Polymer-Based Nanocomposites Modified with Ceramic and Carbon Nanoparticles

The Study of Human Osteoblast-Like MG 63 Cells Proliferation on Resorbable Polymer-Based Nanocomposites Modified with Ceramic and Carbon Nanoparticles

TRPC expression in mesenchymal stem cells

Characterization of oxidized low-density lipoprotein-induced hormesis-like effects in osteoblastic cells

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