TRPC expression in mesenchymal stem cells

Torossian, Frédéric; Bisson, Aurélie; Vannier, Jean‐Pierre; Boyer, Olivier; Lamacz, Marek

doi:10.2478/s11658-010-0031-3

Cited by 15 publications

(12 citation statements)

References 33 publications

(44 reference statements)

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“…TRPV4-mediated Ca 2+ signaling is also a positive regulator of Sox9 and as such, has been shown to promote chondrogenesis 65 and in transducing the mechanical signals that support cartilage extracellular matrix maintenance and joint health 44 , 59 . TRPC1 is expressed during early chondrocyte expansion 66 , as well as being involved in the proliferation of mesenchymal stem cells 67 . We detected time, intensity, and PEMF dosage-dependent up-regulations of both TRPV4 and TRPC1 that closely correlated with the PEMF-induced expression pattern of chondrogenic markers (Fig.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of mesenchymal stem cell chondrogenesis with short-term low intensity pulsed electromagnetic fields

Parate

Franco‐Obregón

Fröhlich

et al. 2017

Sci Rep

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Pulse electromagnetic fields (PEMFs) have been shown to recruit calcium-signaling cascades common to chondrogenesis. Here we document the effects of specified PEMF parameters over mesenchymal stem cells (MSC) chondrogenic differentiation. MSCs undergoing chondrogenesis are preferentially responsive to an electromagnetic efficacy window defined by field amplitude, duration and frequency of exposure. Contrary to conventional practice of administering prolonged and repetitive exposures to PEMFs, optimal chondrogenic outcome is achieved in response to brief (10 minutes), low intensity (2 mT) exposure to 6 ms bursts of magnetic pulses, at 15 Hz, administered only once at the onset of chondrogenic induction. By contrast, repeated exposures diminished chondrogenic outcome and could be attributed to calcium entry after the initial induction. Transient receptor potential (TRP) channels appear to mediate these aspects of PEMF stimulation, serving as a conduit for extracellular calcium. Preventing calcium entry during the repeated PEMF exposure with the co-administration of EGTA or TRP channel antagonists precluded the inhibition of differentiation. This study highlights the intricacies of calcium homeostasis during early chondrogenesis and the constraints that are placed on PEMF-based therapeutic strategies aimed at promoting MSC chondrogenesis. The demonstrated efficacy of our optimized PEMF regimens has clear clinical implications for future regenerative strategies for cartilage.

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Section: Discussionmentioning

confidence: 99%

Enhancement of mesenchymal stem cell chondrogenesis with short-term low intensity pulsed electromagnetic fields

Parate

Franco‐Obregón

Fröhlich

et al. 2017

Sci Rep

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“…In addition, TRPV4 agonist-induced calcium entry can be directly associated with actomyosin network [23] . Transient Receptor Potential Channel 1 (TRPC1) and Transient Receptor Potential Channel 6 (TRPC6) expressed in MSCs [24] might also be cooperating with TRPV4 channels in mediating intracellular calcium signaling in response to mechanical stimuli [25] .…”

Section: Discussionmentioning

confidence: 99%

Prolonged Mechanical Stretch Initiates Intracellular Calcium Oscillations in Human Mesenchymal Stem Cells

Kim

Sun

et al. 2014

PLoS ONE

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Mesenchymal stem cells (MSCs) are a promising candidate for cell-based therapy in regenerative medicine. These stem cells can interact with their mechanical microenvironment to control their functions. External mechanical cues can be perceived and transmitted into intracellular calcium dynamics to regulate various cellular processes. Recent studies indicate that human MSCs (hMSCs) exhibit a heterogeneous nature with a subset of hMSCs lacking spontaneous calcium oscillations. In this study, we studied whether and how external mechanical tension can be applied to trigger and restore the intracellular calcium oscillation in these hMSCs lacking spontaneous activities. Utilizing the fluorescence resonance energy transfer (FRET) based calcium biosensor, we found that this subpopulation of hMSCs can respond to a prolonged mechanical stretch (PMS). Further results revealed that the triggering of calcium oscillations in these cells is dependent on the calcium influx across the plasma membrane, as well as on both cytoskeletal supports, myosin light chain kinase (MLCK)-driven actomyosin contractility, and phospholipase C (PLC) activity. Thus, our report confirmed that mechanical tension can govern the intracellular calcium oscillation in hMSCs, possibly via the control of the calcium permeability of channels at the plasma membrane. Our results also provide novel mechanistic insights into how hMSCs sense mechanical environment to regulate cellular functions.

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“…Weick and co-workers demonstrated that TRPC1 and TRPC4 act as molecular targets for controlling neurite elongation in human embryonic stem cell-derived neurons (80). That MSCs express canonical TRPC1, 2, 4 and 6 mRNA and that TRPC1 may be involved in stem cell proliferation has been recently shown (81).…”

Section: Transient Receptor Potential (Trp) Pathwaysmentioning

confidence: 99%

Calcium signalling in chondrogenesis implications for cartilage repair

Matta¹

2013

Front Biosci

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Undifferentiated mesenchymal stem cells (MSCs) represent an important source for cell-based tissue regeneration techniques that require differentiation towards specific lineages, including chondrocytes. Chondrogenesis, the process by which committed mesenchymal cells differentiate into chondrocytes, is controlled by complex but not yet completely understood mechanisms that involve many components, including intracellular signalling pathways, as well as plasma membrane receptors and ion channels. Some of these signalling components are Ca2+ sensitive. Although the Ca2+-signalling toolkit of undifferentiated MSCs and mature chondrocytes are extensively studied, the adaptation of these components during differentiation and their role in chondrogenesis is not adequately established. In this review, various aspects of Ca2+ signalling are discussed in MSCs and in mature chondrocytes including spatial and temporal aspects, as well as Ca2+ entry and elimination processes, with implications for their involvement in chondrogenesis. A better understanding of these pathways is envisaged to provide a more efficient differentiation of MSCs towards chondrocytes that may lead to the development of better cartilage tissue engineering techniques.

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TRPC expression in mesenchymal stem cells

Cited by 15 publications

References 33 publications

Enhancement of mesenchymal stem cell chondrogenesis with short-term low intensity pulsed electromagnetic fields

Enhancement of mesenchymal stem cell chondrogenesis with short-term low intensity pulsed electromagnetic fields

Prolonged Mechanical Stretch Initiates Intracellular Calcium Oscillations in Human Mesenchymal Stem Cells

Calcium signalling in chondrogenesis implications for cartilage repair

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