The Study of Human Osteoblast-Like MG 63 Cells Proliferation on Resorbable Polymer-Based Nanocomposites Modified with Ceramic and Carbon Nanoparticles

Wiecheć, A.; Stodolak-Zych, Ewa; Frączek-Szczypta, Aneta; Błażewicz, M.; Kwiatek, Wojciech M.

doi:10.12693/aphyspola.121.546

Cited by 6 publications

(3 citation statements)

References 10 publications

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“…No significant difference in doubling time were seen among the surfaces examined (Ti-KC, 31.03 ±6 h and Ti-KF, 33.6 ± 6.4 h), which were similar to that seen (∼29 h) with culture on keratin sponges [23]. While studies have shown 1.3-1.9 times higher doubling times of MG-63 cells cultured on different substrates [37,38], the results of this study indicate that the growth rate of MG-63 cells on all surfaces was similar despite any poor initial cell attachment.…”

Section: Discussionsupporting

confidence: 72%

The effects of keratin-coated titanium on osteoblast function and bone regeneration

Ranjit,

Hamlet,

Shelper

et al. 2024

Biomed. Mater.

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Introduction: Wool derived keratin, due to its demonstrated ability to promote bone formation, has been suggested as a potential bioactive material for implant surfaces. The aim of this study was to assess the effects of keratin-coated titanium on osteoblast function in vitro and bone healing in vivo.Methods: Keratin-coated titanium surfaces were fabricated via solvent casting and molecular grafting. The effect of these surfaces on the attachment, osteogenic gene, and osteogenic protein expression of MG-63 osteoblast-like cells were quantified in vitro. The effect of these keratin-modified surfaces on bone healing over three weeks using an intraosseous calvaria defect was assessed in rodents.Results: Keratin coating did not affect MG-63 proliferation or viability, but enhanced osteopontin, osteocalcin and bone morphogenetic expression in vitro. Histological analysis of recovered calvaria specimens showed osseous defects covered with keratin-coated titanium had a higher percentage of new bone area two weeks after implantation compared to that in defects covered with titanium alone.Conclusions: The keratin-coated surfaces were biocompatible and stimulated osteogenic expression in adherent MG-63 osteoblasts. Furthermore, a pilot preclinical study in rodents suggested keratin may stimulate earlier intraosseous calvaria bone healing. 

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Section: Discussionsupporting

confidence: 72%

The effects of keratin-coated titanium on osteoblast function and bone regeneration

Ranjit,

Hamlet,

Shelper

et al. 2024

Biomed. Mater.

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“…The PLA nanofibres are degrading in a biological environment with the time, and it may be expected to expose the surface of carbon nanotubes. The biological tests indicated that the samples containing carbon nanotubes were not toxic [51]. On the other hand, the presence of CNTs in PLA nanofibres modifies their mechanical and electrical properties.…”

Section: Resultsmentioning

confidence: 97%

Preparation and Characterization of Nanofibrous Polymer Scaffolds for Cartilage Tissue Engineering

Markowski

Magiera

Lesiak

et al. 2015

Journal of Nanomaterials

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Polymer substrates obtained from poly(lactic acid) (PLA) nanofibres modified with carbon nanotubes (CNTs) and gelatin (GEL) for cartilage tissue engineering are studied. The work presents the results of physical, mechanical, and biological assessment. The hybrid structure of PLA and gelatine nanofibres, carbon nanotubes- (CNTs-) modified PLA nanofibres, and pure PLA-based nanofibres was manufactured in the form of fibrous membranes. The fibrous samples with different microstructures were obtained by electrospinning method. Microstructure, physical and mechanical properties of samples made from pure PLA nanofibres, CNTs-, and gelatin-modified PLA-nanofibres were studied. The scaffolds were also testedin vitroin cell culture of human chondrocytes collected from patients. To assess the influence of the nanofibrous scaffolds upon chondrocytes, tests for cytotoxicity and genotoxicity were performed. The work reveals that the nanofibrous structures studied were neither genotoxic nor cytotoxic, and their microstructure, physical and mechanical properties create promising scaffolds for potential use in cartilage repairing.

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“…It was previously reported that bone marrow‐derived stromal cells (BMSCs) on the composite scaffolds differentiated down the osteogenic lineage and expressed high levels of the bone marker ALP. It was confirmed that the PCL/CNT nanocomposites are appropriate for the growth and proliferation of the human osteoblast‐like cells []. Biodegradation experiments showed that PCL still holds its biodegradability and the CNTs hold on to their inherently tube‐like characteristic.…”

Section: Hybrid Bone Scaffolds For Maxillofacial Defects and Tissue Ementioning

confidence: 92%

Unraveling the mechanical strength of biomaterials used as a bone scaffold in oral and maxillofacial defects

Prasadh

Wong

2018

Oral Science International

212

125

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Three‐dimensional printed natural and synthetic biomaterials have evolved as gold standards for tissue engineering scaffolds in recent trends owing to their superior role in hard tissue regeneration. The major drawback of these scaffolds is their relatively poor mechanical strength. Another key consideration in the design of the scaffolds is the difficulty in replicating the complex structural composition of hard tissues such as bones, and its structure cannot be reproduced with a single material that provides a limited range of properties. Sufficient mechanical strength is provided by the structure required for the replacement tissue. The mechanical properties of the scaffold play an important role in many applications of tissue engineering. Therefore, is it sufficient to withstand the force with only a single material used for the scaffold? There are many materials such as natural resin, synthetic resin, and polymers. They are used in combination to fulfill the function and to act as a kingpin by solving their drawbacks. The added material is not only superior in mechanical strength but also compatible with the tissues surrounding the implant, promoting cell adhesion and gradually degrading rather than intoxicating the patient. This review focuses on the various biomaterials used as scaffolds for critical size defects and the aftermath in their mechanical properties.

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The Study of Human Osteoblast-Like MG 63 Cells Proliferation on Resorbable Polymer-Based Nanocomposites Modified with Ceramic and Carbon Nanoparticles

Cited by 6 publications

References 10 publications

The effects of keratin-coated titanium on osteoblast function and bone regeneration

The effects of keratin-coated titanium on osteoblast function and bone regeneration

Preparation and Characterization of Nanofibrous Polymer Scaffolds for Cartilage Tissue Engineering

Unraveling the mechanical strength of biomaterials used as a bone scaffold in oral and maxillofacial defects

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