Canine visceral leishmaniosis: A comparative analysis of the EIE-leishmaniose-visceral-canina-Bio-Manguinhos and the IFI-leishmaniose-visceral-canina-Bio-Manguinhos kits

Lira, Rodrigo Araújo; Cavalcanti, Milena Paiva; Nakazawa, Mineo; Ferreira, Antônio G. P.; Silva, E.D.; Abath, Frederico G. C.; Alves, Lêucio Câmara; Souza, Wayner Vieira de; Gomes, Yara M.

doi:10.1016/j.vetpar.2005.12.020

Cited by 62 publications

(53 citation statements)

References 15 publications

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“…OLIVEIRA et al 21 demonstrated 90% sensitivity and 100% specificity for ELISA and 40% sensitivity and 98.6% specificity for IFAT. However, LIRA et al 14 observed no significant difference between the two tests. Although no differences in the sensitivity of the techniques were observed in the present study, specificity was significantly higher for ELISA and the positive predictive values for the IFAT were very low.…”

Section: Discussionmentioning

confidence: 90%

Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples

Figueiredo

Madeira

Nascimento

et al. 2010

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe Brazilian Ministry of Health recommends the culling and euthanasia of dogs with a positive serological test for canine visceral leishmaniasis (CVL). In the Municipality of Rio de Janeiro, the technique used for the diagnosis of CVL is the indirect fluorescent antibody test (IFAT), using blood samples eluted on filter paper (eluate). A dog survey was conducted over a period of one year in the region of Carapiá, in order to evaluate the diagnosis of CVL in this region. All animals underwent clinical examination, and blood samples (serum and eluate) were collected for analysis by enzyme immunoassay (ELISA) and IFAT. A skin biopsy was obtained for parasitological examination (culture). A total of 305 animals were studied and Leishmania chagasi was isolated from nine animals. Sensitivity and specificity were 100% and 96.6% for ELISA, respectively, 100% and 65.5% for IFAT (cut-off at a 1:40 dilution), 100% and 83.4% for IFAT (cut-off at a 1:80 dilution), and 22.2% and 97.0% for eluate IFAT. In conclusion, ELISA was the best tool for the diagnosis of CVL among the serological techniques tested. The present results suggest the need for a better evaluation of filter paper IFAT as the only diagnostic method for CVL in the Municipality of Rio de Janeiro.

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Section: Discussionmentioning

confidence: 90%

Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples

Figueiredo

Madeira

Nascimento

et al. 2010

Rev. Inst. Med. trop. S. Paulo

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“…antibodies. There are several serological methods used for detection of Leishmania sp.-specific antibodies, including the indirect fluorescent antibody test (IFAT), complement fixation, direct agglutination, enzyme-linked immunosorbent assay, and ICT dipstick tests (Rosypal et al, 2003;Lira et al, 2006). The IFAT is the most commonly used of all serological assays and represents the ''gold standard'' with which other serological tests are compared (Rosypal et al, 2003).…”

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confidence: 99%

Surveillance for Antibodies to Leishmania spp. in Dogs from Sri Lanka

Rosypal¹,

Tripp²,

Kinlaw³

et al. 2010

Journal of Parasitology

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“…The Enzyme-linked immunosorbent assay (EIE) 1 and Indirect Immunofluorescence (IFI) 1 were used to analyze the seroreactivity of each sample [23]. For these assays, the cutoff points were considered a titer higher than 1:40.…”

Section: Serological Testsmentioning

confidence: 99%

Catalase and Glutathione Peroxidase in Dogs Naturally Infected by Leishmania infantum

Lopes-Neto

Santos

Lima

et al. 2016

Acta Scientiae. Vet.

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Background: Canine leishmaniasis (CanL) is caused by an obligatory intracellular parasite of Leishmania genus that affects organs and tissues. Several studies evaluate the role of reactive oxygen species (ROS) in the pathogenesis of many diseases. The overproduction of ROS on infectious diseases can induce an imbalance between oxidants and antioxidants at cellular or systemic level. Thus, the aim of this study was to evaluate the activity of antioxidant enzymes in CanL.Materials, Methods & Results: Females (n = 17) and males (n = 10), at different ages and with different weight, were selected for this study. Dogs were divided into two groups according classical clinical signs and sorological test to CanL. Animals were considered infected based on indirect immunofluorescent assay and ELISA titration ≥ 1:40. Group B (n = 15) composed by positive dogs to CanL from Zoonosis Control Center of Fortaleza (Ceará, Brazil) and group A (n = 12) was composed by dogs from private kennel that were serologically negative to L. infantum and had absence of clinical signs to CanL. Blood sample were collected for evaluation of hematological and biochemical parameters and glutathione peroxidase (GPx) and catalase (CAT) enzymatic activity. Data were analyzed by Student’s t-test and Pearson correlation coefficient (P < 0.05). Total proteins (TP, mg/dL) and alkaline phosphatase (ALP, U/L) were increased (P < 0.05) on group B (8.2 ± 1.2; 165.4 ± 46.4) when compared to group A (6.5 ± 1.1; 109.1 ± 38.3), respectively. Hemoglobin (Hb; g/dL) and hematocrit (Hct; %) were decreased (P < 0.05) on Group B (14.7 ± 1.8; 48.2 ± 5.7) when compared to group A (16.5 ± 1.3; 52.1 ± 2.4), respectively. Group B presented CAT (U/g Hb) and GPx (mU/mg Hb) lower (189.4 ± 90.4; 3,609.6 ± 1,569.1) than group A (326.6 ± 104.5; 5,055.6 ±1,569.1), respectively (P < 0.001). Positive correlation was observed between RBC and CAT; however, it was not significant.Discussion: Organisms require a good defense system in order to revert the overproduction of free radicals and consequently the injuries caused by them. This is possible through the production of antioxidant agents, which act on oxidative prevention and on tissue and cellular regeneration, by taking the reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) enzymes in the frontline. Erythrocyte changes promoted by CanL suggest possible correlation between anemia and the appearance of clinical signs, which in many cases is not seen. Erythrocytes contain SOD, CAT and GPx enzymes, thus, changes in these cells will reflect on the activity of these enzymes. In our results only CAT showed positive correlation with erythrocyte count, however it was not significant. GPx activity was lower (P < 0.001) in infected dogs than control group. This result agrees with another study, which showed a decrease in GPx levels in CanL, although it was not significant. However, it was found a positive correlation (P < 0.001) between erythrocytes and GPx activity and between hemoglobin and GPx activity in animals with leishmaniasis. These results suggest that the reduction in detoxification activity can be related to the decrease in erythrocyte count and that the GPx activity depends on the control mechanism of the antioxidant system in CanL. Furthermore, this result could be associated with decrease of blood cell count in animals with CanL, once GPx is an erythrocyte enzyme, which plays an important role in hemoglobin protection against oxidative damage. This study was carried out in naturally infected dogs with L. infantum. In conclusion, CAT and GPx activities are relate to oxidative stress induced by L. infantum infection and can be used as biomarkers on CanL.

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Canine visceral leishmaniosis: A comparative analysis of the EIE-leishmaniose-visceral-canina-Bio-Manguinhos and the IFI-leishmaniose-visceral-canina-Bio-Manguinhos kits

Cited by 62 publications

References 15 publications

Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples

Canine visceral leishmaniasis: study of methods for the detection of IgG in serum and eluate samples

Surveillance for Antibodies to Leishmania spp. in Dogs from Sri Lanka

Catalase and Glutathione Peroxidase in Dogs Naturally Infected by Leishmania infantum

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