Candidate Reference Method for the Quantification of Circulating 25-Hydroxyvitamin D3 by Liquid Chromatography–Tandem Mass Spectrometry

Vogeser, Michael; Kyriatsoulis, Apostolos; Huber, E.M.; Kobold, Uwe

doi:10.1373/clinchem.2004.031831

Cited by 119 publications

(82 citation statements)

References 17 publications

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“…[10] Another large study including 310 genotype 1 patients also found no association. [24] In 2 studies that frankly showed no association of response with vitamin D level [our current study and Kitson et al,2013] vitamin D levels were measured by LC-MS/MS methodology, [10,32] avoiding fallacies that may be induced by other commercially available kits. Another noticeable difference between the studies is the prevalence of advanced fibrosis.…”

Section: Discussionmentioning

confidence: 96%

Vitamin D Receptor FokI Gene Polymorphism Predicted Poor Response to Treatment in Chronic HCV Genotype 4

Shehab¹,

Sabry²,

Mukhtar³

et al. 2016

Int J Biomed

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The aim of this study was to investigate the association between a genetic polymorphism of the vitamin D receptor (VDR) and antiviral responses in Egyptian patients with chronic hepatitis C virus genotype 4 (HCV-4). Methods: Our study enrolled 100 HCV-4 patients who received pegylated interferon alpha-2a (pegIFNα-2a) and ribavirin for 48 weeks. Patients were divided into 2 groups according to their response to therapy: 50 were responders, and 50 were nonresponders. All HCV-4 patients were further subjected to the following laboratory tests: HCV-RNA using quantitative PCR, vitamin D level using ELISA and VDR genotype using PCR-RFLP assays, and abdominal ultrasonography.Results: There was a statistically significant difference in the frequency of the VDR polymorphism (FokI rs10735810) between responders (FF:60%, Ff:16%, ff:24%) and non-responders (FF:10%, Ff:26%, ff:64%) (P<0.001). There was a statistically significant association between VDR polymorphism with higher ALT levels (ff: 63.2±30

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Section: Discussionmentioning

confidence: 96%

Vitamin D Receptor FokI Gene Polymorphism Predicted Poor Response to Treatment in Chronic HCV Genotype 4

Shehab¹,

Sabry²,

Mukhtar³

et al. 2016

Int J Biomed

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“…The Elecsys assay is standardized against a modified version of the LC-MS/MS method published by Vogeser et al (22) with traceability to the reference material NIST SRM 2972, which consists of two ethanolic solutions of either 25-OHD2 or 25-OHD3 that are used to calibrate chromatographic methods (5,21,30). In addition, the performance of the in house LC-MS/MS was monitored by using NIST SRM 972 (vitamin D in human serum), which is the first certified reference material for quality-control use with officially assigned target values (5,18,19,30).…”

Section: Discussionmentioning

confidence: 99%

“…The Penzberg LC-MS/MS method utilizes an HPLC system (Agilent 1100 series) with additional binary pump for online-SPE (solidphase extraction) in conjunction with a triple quadrupole mass spectrometer with APCI source (atmospheric pressure chemical ionization; Thermo Scientific TSQ Quantum Ultra EMR). The calibration is performed against NIST standards as described above, using a modified version of a previously published LC-MS/MS method (22). The HPLC system was calibrated with the Chromsystems Serum Calibration Standard D3/D3 nr 38033.…”

Section: Method-correlation Studiesmentioning

confidence: 99%

Automated Competitive Protein‐Binding Assay for Total 25‐OH Vitamin D, Multicenter Evaluation and Practical Performance

Wielders

Carter²,

Eberl

et al. 2014

Clinical Laboratory Analysis

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Background:The Roche Elecsys Vitamin D Total competitive protein-binding assay uses recombinant vitamin D binding protein for measuring 25-hydroxyvitamin D (25-OHD), which is different from commonly used antibody assays. Methods: The assay, standardized against LC-MS/MS, was tested at four sites. Evaluation included precision; between-laboratory variability; functional sensitivity; correlation to LC-MS/MS, HPLC, and immunoassays; as well as robustness, traceability, and EQAS performance. Results: Precision testing showed within-run coefficient of variations (CVs) of ࣘ7%, within-laboratory CVs of <9.5%, between-laboratory precision CVs of ࣘ10.1%, and a functional sensitivity below 9.8 nmol/l (at CV 12.9%). The assay showed equivalent 25-OHD levels for matched serum and plasma samples, good reagent lot-to-lot consistency in pooled sera over time, and good agreement with HPLC (relative bias −8.8%). Comparison with LC-MS/MS methods yielded relative biases of −15.4, −13.5, −10.2, and 3.2%. Comparison against immunoassays showed a relative bias of 14.5% (DiaSorin Liaison) and −58.2% (IDS-iSYS). The overall mean results in 2 years DE-QAS was 102% of the ALTM. In a certified reference patient panel, the average bias was <4% for the sum of 25-OHD2 and 25-OHD3. Conclusion: The Elecsys Vitamin D Total assay demonstrated good overall performance and is, according to present standards, very suitable for automated measurement of 25-OHD.

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“…None of the commercial immunoassays are, therefore, aligned to SRM 972. Several candidate reference methods for accurate and sensitive 25-OHD measurement have also been published in the recent years (19)(20)(21).…”

Section: Introductionmentioning

confidence: 99%

Analytical methods and performance of the immunoassay methods for determination of vitamin d in comparison to mass spectrometry / Analitičke metode i izvođenje imunometrijskih određivanja vitamina d u poređenju sa masenom spektrometrijom

Babić¹

2012

Journal of Medical Biochemistry

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Summary: Demand for vitamin D testing has been on a constant rise worldwide, partially due to mounting evidence linking vitamin D status to overall health and well-being. Currently available assays measure 25-hydroxy vitamin D (25-OHD), a major circulating form of vitamin D. Available methodologies include immunoassays and mass spectrometry based methods (LC-MS/MS). Until recently, the only immunoassays available for diagnostic use in the US have been DiaSorin radioimmunoassay (RIA) and an automated immunoassay on a LIAISON ® platform. Within the last year, Siemens and Abbott successfully launched immunoassays for determination of total vitamin D on their respective automated platforms, Centaur ® and ARCHITECT ® . Development of robust and precise Vitamin D immunoassays has historically been plagued with difficulty. One of the major challenges is development of specific antibodies against such a small antigen. Vitamin D is also highly hydrophobic molecule predominantly bound to vitamin D binding protein (DBP). It is likely, therefore, that immunoassays might be affected to varying extent by the DBP concentration. Adoption of LC-MS/MS into clinical laboratories has enabled development of accurate and almost fully automated methods that could handle increasing volume demands, especially in large volume reference laboratories. Smaller to mid-size hospital laboratories as well as physician offices have neither funds nor technical expertise to implement LC-MS/MS based testing. Our laboratory at the University of Chicago Medical Center has also seen increase in vitamin D volume and currently performs close to 20,000 25-OHD assays per year. We have recently developed an LC-MS/MS method for quantitation of 25-OHD 2 (obtained from plant sources) and 25-OHD 3 (endogenous and animal sources). Prior to acquisition of LC-MS/MS Kratak sadr`aj: Broj zahteva za odre|ivanjem vitamina D je u konstantnom porastu {irom sveta, delom zbog sve vi{e dokaza koji povezuju status vitamina D sa op{tim zdravljem. Trenutno raspolo`ivim testovima odre|uje se 25-hidroksi vitamin D (25-OHD), glavni oblik vitamina D u cirkulaciji. Po stoje}e metodologije uklju~uju imunometrijska odre |i vanja i tehnike zasnovane na masenoj spektrometriji (LC-MS/MS). Do nedavno, jedine raspolo`ive imunometrijske metode kori{}ene za dijagnostiku u SAD su bile DiaSorin radioimunoodre|ivanje (RIA) i automatizovano imuno odre|ivanje na LIAISON ® platformi. U toku pro{le godine Siemens i Abbott su uspe{no lansirali imunometrijske testove za odre|ivanje ukupnog vitamina D na svojim odgova raju}im automatizovanim platformama, Centaur ® i ARCHI-TECT ® . Razvoj robusnih i preciznih imunometrijskih testova za odre|ivanje vitamina D su, istorijski gledano, pratili problemi. Jedan od najve}ih izazova je razvoj specifi~nih antitela protiv malog antigena. Vitamin D je tako|e jako hidrofoban molekul predominantno vezan za vitamin D vezu ju}i protein (DBP). Stoga postoji verovatno}a da na imuno odre|ivanja u razli~itom stepenu mo`e da uti~e koncentracija DBP. Uvo|enje LC-MS/MS u...

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Candidate Reference Method for the Quantification of Circulating 25-Hydroxyvitamin D3 by Liquid Chromatography–Tandem Mass Spectrometry

Cited by 119 publications

References 17 publications

Vitamin D Receptor FokI Gene Polymorphism Predicted Poor Response to Treatment in Chronic HCV Genotype 4

Vitamin D Receptor FokI Gene Polymorphism Predicted Poor Response to Treatment in Chronic HCV Genotype 4

Automated Competitive Protein‐Binding Assay for Total 25‐OH Vitamin D, Multicenter Evaluation and Practical Performance

Analytical methods and performance of the immunoassay methods for determination of vitamin d in comparison to mass spectrometry / Analitičke metode i izvođenje imunometrijskih određivanja vitamina d u poređenju sa masenom spektrometrijom

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