J. Clin. Lab. Anal. volume 29, issue 6, P451-461 2014 DOI: 10.1002/jcla.21793 View full text
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Jos PM Wielders, Graeme F. Carter, Heike Eberl, Gary Morris, Heinz Jürgen Roth, Christian Vogl

Abstract: Background:The Roche Elecsys Vitamin D Total competitive protein-binding assay uses recombinant vitamin D binding protein for measuring 25-hydroxyvitamin D (25-OHD), which is different from commonly used antibody assays. Methods: The assay, standardized against LC-MS/MS, was tested at four sites. Evaluation included precision; between-laboratory variability; functional sensitivity; correlation to LC-MS/MS, HPLC, and immunoassays; as well as robustness, traceability, and EQAS performance. Results: Precision te…

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