Looking beyond linear regression and Bland-Altman plots: a comparison of the clinical performance of 25-hydroxyvitamin D tests

Karvaly, Gellért Balázs; Mészáros, Katalin; Kovács, K; Patócs, Attila; Sipák, Zoltán; Vásárhelyi, Barna

doi:10.1515/cclm-2016-0536

Cited by 5 publications

(1 citation statement)

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“…Dowling et al had reported over-estimation of 25(OH)D measurements in the Roche assay due to the cross-reactivity of 24,25(OH) 2 D present in the samples [8]. Karvaly et al had demonstrated the extent of the discrepancies between seven different methods on the interpretation of vitamin D status and found the DiaSorin Liaison had identified significantly more hypovitaminosis cases, whereas fewer cases were detected by IDS-iSYS D assay [9]. Disparity can also occur in specific population groups; Hara et al had reported falsely high 25(OH) D in serum samples from in infants and postpartum women produced by the Diasorin radioimmunoassay (RIA).…”

Section: Immunoassays For 25(oh)dmentioning

confidence: 99%

Vitamin D Measurement, the Debates Continue, New Analytes Have Emerged, Developments Have Variable Outcomes

et al. 2019

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The demand for measurement of vitamin D metabolites for clinical diagnosis and to advance our understanding of the role of vitamin D in human health has significantly increased in the last decade. New developments in technologies employed have enabled the separation and quantification of additional metabolites and interferences. Also, developments of immunoassays have changed the landscape. Programmes and materials for assay standardisation, harmonisation and the expansion of the vitamin D external quality assurance scheme (DEQAS) with the provision of target values as measured by a reference measurement procedure have improved standardisation, quality assurance and comparability of measurements. In this article, we describe developments in the measurement of the commonly analysed vitamin D metabolites in clinical and research practice. We describe current analytical approaches, discuss differences between assays, their origin, and how these may be influenced by physiological and experimental conditions. The value of measuring metabolites beyond 25 hydroxyvitamin D (25(OH)D), the marker of vitamin D status, in routine clinical practice is not yet confirmed. Here we provide an overview of the value and application of the measurement of 1,25 dihydroxyvitamin D, 24,25 dihydroxyvitamin D and free 25OHD in the diagnosis of patients with abnormalities in vitamin D metabolism and for research purposes.

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Section: Immunoassays For 25(oh)dmentioning

confidence: 99%