Candida albicans adherence to a human oesophageal cell line

Abstract

The oesophageal epithelium appears to be one of the primary cell targets of Candida albicans in AIDS patients. To study this interaction, we have established an in vitro adherence assay using a human epithelial oesophageal cell line (HET1-A). When yeast cells were grown in 500 mM D-galactose, adherence increased significantly over cultures prepared in 500 mM D-glucose. kDa was observed in Aurodye-stained SDS-PAGE gels and in Western blots using an immunoglobulin fraction (IgG) prepared from sera of rabbits infected with the organism. These studies demonstrate that C. albicans adheres to human oesophageal cells and may utilize cell surface proteins whose synthesis is nutritionally regulated.

Antigenicity of Cell Wall Mannans of Candida albicans NIH A-207 Strain Cells Cultured in Galactose-Added Yeast Nitrogen Base Medium

“…It has been known that the growth in a high-galactose medium (YNB-Gal) promotes the synthesis of an mannoprotein adhesin, resulting in increased adhesion to the various animall cells. 2,3,5) The results of the present YNB-Gal study seem to be useful for determining the structure and the function of the mannoprotein adhesin. Agglutination was scored from high (ϩϩϩ) to very low (Ϯ), and no agglutination (Ϫ).…”

“…In 1981, Douglas et al reported that yeast nitrogen base medium (Difco) containing 500 mM galactose (YNB-Gal) is useful for the studies of the adhesion to acrylic surfaces 1) and buccal epithelial cells 2,3) of C. albicans, and recently, of the biofilm formations. 4) They and others 5) mentioned that the protein portrion of the mannoprotein adhesin of the yeast is more important than the carbohydrate moiety. On the other hand, in separate experiments, several authors have shown that the cell-wall mannan parts are implicated to have at least some adhesive function to the host cells.…”

Adherence of clinical isolates of Saccharomyces cerevisiae to buccal epithelial cells

“…Previous investigations into factors affecting the adherence of C. albicans have shown that the level of adherence to epithelial tissue is in uenced by the carbon source in which the yeast is cultured [16,17]. C. albicans 10231, a commercially available Bakers' yeast product and four of the clinical S. cerevisiae isolates (1227, 1464, 128 and 436) were grown to the stationary phase in YEPD, YEPGal or YEPSuc and adherence assays were performed as described.…”

Oral Colonization By Candida Albicans

“…The purified fragment inhibited binding to BECs by up to 80%. Binding to an esophageal cell line (Het-l) is partially mediated by a lectin-like interaction (Enache et al, 1996). Yeast cells grown on galactose adhere better than those grown on glucose, and GlcNAc or glucosamine reduce binding by about 40%.…”

“…Yeast cells grown on galactose adhere better than those grown on glucose, and GlcNAc or glucosamine reduce binding by about 40%. A 190-kDa glycoprotein detected in cell wall extracts of galactosegrown cells was postulated to be responsible for the increased adherence (Enache et al, 1996). The serotype A determinant, factor 6, of C. albicans mannan has also been implicated in epithelial adherence (Miyakawa et al, 1992).…”