Adherence of clinical isolates of Saccharomyces cerevisiae to buccal epithelial cells

Murphy, Alan; Kavanagh, Kevin

doi:10.1080/mmy.39.1.123.127

Cited by 15 publications

(5 citation statements)

References 29 publications

(31 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Results showed that both clinical (vaginal) and nonclinical (dietetic and biotherapeutic) isolates possess a limited, but detectable ability to adhere to endothelial, and particularly to epithelial cell lines, as compared to the faecal ** */** isolate and the reference wine strain, which were much less adherent, and to C. albicans, which possess a higher adherence potential. A similar result was reported for the adhesion of S. cerevisiae clinical and non-clinical isolates to Caco-2 and buccal epithelial cells [30,46]. This ability to adhere to host cells can be considered as a virulence factor contributing to the pathogenicity of S. cerevisiae isolates.…”

Section: Discussionsupporting

confidence: 81%

In Vivo and In Vitro Studies on Virulence and Host Responses to Saccharomyces cerevisiae Clinical and Non-Clinical Isolates

Yáñez¹,

Murciano²,

Llopis³

et al. 2009

TOMYCJ

View full text Add to dashboard Cite

We have studied the virulence and host responses to several clinical and non-clinical Saccharomyces cerevisiae isolates: two vaginal isolates (60, 61), one isolate from faeces (20), a brewer's yeast isolate used in dietetics (D14), one S. boulardii isolate from a commercial probiotic product, and a reference natural wine yeast (CECT 10431). Hematogenously disseminated infection in a mouse model demonstrated that four isolates (all, except 20 and 10431) were able to colonize preferentially the brain, as well as kidney and spleen, to a lesser extent, of immunocompetent mice. In vitro adhesion assays to epithelial and endothelial cell lines also showed an increased adherence ability of strains 60, 61, D14 and S. boulardii. In vitro cytokine production assays by RAW 264.7 murine macrophages challenged with yeasts showed a relative increased production of TNF-in response to the 20 and 10431 strains; viability of RAW cells after coculture was similar in all cases (2-5% non-viable cells) except for 60 strain (11% non-viable cells). In vitro phagocytosis assays of yeasts by RAW cells showed that two isolates (D14 and particularly S. boulardii) were engulfed less efficiently. These results point out that S. cerevisiae isolates, from both clinical and non-clinical (dietetic and probiotic) origin, may vary in the expression of putative virulence factors contributing to their ability to develop the infectious process.

show abstract

Section: Discussionsupporting

confidence: 81%

In Vivo and In Vitro Studies on Virulence and Host Responses to Saccharomyces cerevisiae Clinical and Non-Clinical Isolates

Yáñez¹,

Murciano²,

Llopis³

et al. 2009

TOMYCJ

View full text Add to dashboard Cite

show abstract

“…The number of IPEC-J2 cells that grew to 70% confluence was estimated based on the counting results of IPEC-J2 cells that were sub-cultured in parallel. The proportion of K. slooffiae numbers to IPEC-J2 cell numbers was chosen according to previously described co-culture procedures [ 50 – 54 ]. The representative pictures for the co-culture of K. slooffiae and IPEC-J2 cells were shown in Additional file 4 : Fig.…”

Section: Methodsmentioning

confidence: 99%

Core-predominant gut fungus Kazachstania slooffiae promotes intestinal epithelial glycolysis via lysine desuccinylation in pigs

Chen²,

Hou

et al. 2023

Microbiome

View full text Add to dashboard Cite

Background Gut fungi are increasingly recognized as important contributors to host physiology, although most studies have focused on gut bacteria. Post-translational modifications (PTMs) of proteins play vital roles in cell metabolism. However, the contribution of gut fungi to host protein PTMs remains unclear. Mining gut fungi that mediate host protein PTMs and dissecting their mechanism are urgently needed. Results We studied the gut fungal communities of 56 weaned piglets and 56 finishing pigs from seven pig breeds using internal transcribed spacer (ITS) gene amplicon sequencing and metagenomics. The results showed that Kazachstania slooffiae was the most abundant gut fungal species in the seven breeds of weaned piglets. K. slooffiae decreased intestinal epithelial lysine succinylation levels, and these proteins were especially enriched in the glycolysis pathway. We demonstrated that K. slooffiae promoted intestinal epithelial glycolysis by decreasing lysine succinylation by activating sirtuin 5 (SIRT5). Furthermore, K. slooffiae-derived 5′-methylthioadenosine metabolite promoted the SIRT5 activity. Conclusions These findings provide a landscape of gut fungal communities of pigs and suggest that K. slooffiae plays a crucial role in intestinal glycolysis metabolism through lysine desuccinylation. Our data also suggest a potential protective strategy for pigs with an insufficient intestinal energy supply.

show abstract

“…A growing number of S. cerevisiae infections in humans have recently been reported (14). As a result, S. cerevisiae is also regarded as an emerging opportunistic pathogen that can cause clinically relevant infections in different patient types and body sites (15)(16)(17). One clinical strain (YJM145), derived from a yeast isolated from an AIDS patient with S. cerevisiae pneumonia (18), has been studied extensively as a model for fungal infections (19)(20)(21)(22)(23)(24).…”

mentioning

confidence: 99%

Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789

McCusker

Hyman

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

243

291

View full text Add to dashboard Cite

We sequenced the genome of Saccharomyces cerevisiae strain YJM789, which was derived from a yeast isolated from the lung of an AIDS patient with pneumonia. The strain is used for studies of fungal infections and quantitative genetics because of its extensive phenotypic differences to the laboratory reference strain, including growth at high temperature and deadly virulence in mouse models. Here we show that the Ϸ12-Mb genome of YJM789 contains Ϸ60,000 SNPs and Ϸ6,000 indels with respect to the reference S288c genome, leading to protein polymorphisms with a few known cases of phenotypic changes. Several ORFs are found to be unique to YJM789, some of which might have been acquired through horizontal transfer. Localized regions of high polymorphism density are scattered over the genome, in some cases spanning multiple ORFs and in others concentrated within single genes. The sequence of YJM789 contains clues to pathogenicity and spurs the development of more powerful approaches to dissecting the genetic basis of complex hereditary traits.comparative genomics ͉ genome architecture ͉ introgression ͉ lateral gene transfer T here is extensive genetic and phenotypic diversity within species. Determining which of the vast amounts of sequence differences that are found among individuals of a species contribute to heritable traits will allow diseases to be tackled at the molecular level and aid in the development of novel therapies. Saccharomyces cerevisiae, commonly known as baker's or brewer's yeast, plays a central role in food production and is one of the most studied genetic model species. It is not only widely used in biotechnology but also is a powerful model system that has been applied to identify multigenetic factors of hereditary traits (1-7). The genome sequence of one laboratory strain, a derivative of S288c, was the first genome of a free-living eukaryotic organism to be sequenced (8). Over the last 10 years, this genome has served as the reference for the S. cerevisiae species and has catalyzed the development of whole-genome approaches to biology (9, 10). Despite frequent laboratory use of alternative strains, sequence information for S. cerevisiae beyond the domesticated strain S288c has been fragmentary. S288c, which originated from a strain isolated from a rotten fig, was chosen for sequencing because it possesses properties that make it easy to work with, such as minimal colony morphology switching, consistent growth rates in glucose media, and no flocculence (11). At several loci, S288c contains polymorphisms not found in natural isolates, which could be hallmarks of domestication (12,13). A growing number of S. cerevisiae infections in humans have recently been reported (14). As a result, S. cerevisiae is also regarded as an emerging opportunistic pathogen that can cause clinically relevant infections in different patient types and body sites (15)(16)(17). One clinical strain (YJM145), derived from a yeast isolated from an AIDS patient with S. cerevisiae pneumonia (18), has been studied extensively as ...

show abstract

Adherence of clinical isolates of Saccharomyces cerevisiae to buccal epithelial cells

Cited by 15 publications

References 29 publications

In Vivo and In Vitro Studies on Virulence and Host Responses to Saccharomyces cerevisiae Clinical and Non-Clinical Isolates

In Vivo and In Vitro Studies on Virulence and Host Responses to Saccharomyces cerevisiae Clinical and Non-Clinical Isolates

Core-predominant gut fungus Kazachstania slooffiae promotes intestinal epithelial glycolysis via lysine desuccinylation in pigs

Genome sequencing and comparative analysis of Saccharomyces cerevisiae strain YJM789

Contact Info

Product

Resources

About