The cells of Candida albicans NIH A-207 strain (A-strain) cultivated in YSLM at high temperatures (37 and 4O'C) did not undergo agglutination with the factor sera 4, 5, and 6 in a commercially available factor serum kit, 'Candida Check', and formed a grape-like shape. The mannans isolated from the cells had lost their reactivity against the factor sera in ELISA. It was also revealed by 'H NMR analysis that the maxmans contained neither a phosphate group nor ap-1,2-linked mannopyranose unit, although these mannans increased the non-reducing terminal a-1,3-linked mannopyranose unit. The cells and the mannans prepared by cultivation at such high temperatures followed by 27°C in the same medium entirely recovered the reactivity with the factor sera.
To clarify the pathogenicity and the pathogenic factors of Candida tropicalis strains, five strains, IFO 0199, IFO 0587, IFO 0589, IFO 1400, and IFO 1647, of C. tropicalis were tested for their lethality to mice, adherence to Hela cells, hydrophobicity, cell growth under acidic conditions (pH 2.0-5.9), and sucrose assimilation using C. albicans NIH A-207 strain as reference. The pathogenicity for mice of all strains was observed in the increasing order IFO 1400؍IFO 0589, IFO 0587, IFO 1647؍NIH A-207, and IFO 0199. The pathogenicity for mice by all the tested C. tropicalis strains was not correlated with the adherence, the hydrophobicity, or the cell growth. On the other hand, the pathogenicity correlated well with the sucrose assimilation ability. These results show that the pathogenic mechanisms of the C. tropicalis strains were different from those of the C. albicans strains.
The cell wall mannans of two Candida albicans serotype A strains, NIH A-207 and J-1012 (abbreviated as A and J strains, respectively), cultured in yeast extract-Sabouraud liquid medium at pH 2.0, contained neither a phosphate group nor a I-1,2-linked mannopyranose unit (H.
In order to clarify the pathogenicity and the pathogenic factors of various Candida species strains, three strains, NIH A-207 and J-1012 (serotype A), and NIH B-792 (serotype B) of Candida albicans and two strains, ATCC 20408 (karyotype II) and ATCC 36232 (karyotype I) of C. stellatoidea, a synonym for C. albicans, were tested for their lethality to mice, adherence to Hela cells, hydrophobicity, and cell growth under acidic conditions, pH 2.0-5.9. The pathogenicity for mice of all the strains was observed in the order NIH B-792, ATCC 36232, J-1012, NIH A-207, and ATCC 20408. The pathogenicity for mice by all the strains used was well correlated with adherence to the Hela cells, the hydrophobicity, and the cell growth under the acidic condition, pH 2.0. These results emphasize that these specific properties of the C. albicans and C. stellatoidea strains play an important role in the pathogenesis of candidosis.
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