Can ‘mild’ periodate oxidation be used for the specific histochemical identification of sialic acid residues?

Volz, D.; Reid, P. E.; Park, C. M.; Owen, David A.; Dunn, William L.; Ramey, C. W.

doi:10.1007/bf01675200

Cited by 12 publications

(3 citation statements)

References 15 publications

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“…However, it should be interpreted with caution as there is evidence of goblet cell sialomucin heterogeneity within normal rectal mucosa (Lev et al 1985, Muto et al 1985, Katsuyama et al 1985, Sugihara & Jass 1986) and adenocarcinoma (Montero & Segura 1980, Katsuyama et al 1985, and there is possible laboratory variation (Reid et al 1984). Further work with more sensitive PAS-based methods to differentiate N-acylated, 0-acylated and neutral mucins is necessary (Buk & Filipe 1986, Volz et al 1986), but initial results have been disappointing ).…”

Section: Discussionmentioning

confidence: 99%

Mucin profiles in ulcerative colitis with dysplasia and carcinoma

1988

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Mucin secretion was assessed in Crohn's colitis, in ulcerative colitis with regeneration, dysplasia and carcinoma and in non-colitic adenocarcinoma. The high iron diamine-alcian blue (HID-AB) and periodate borohydride-saponification periodic acid Schiff (PB-KOH-PAS) techniques were used to demonstrate sulphomucins and sialomucins, and O-acylated sialomucins respectively. There was mucosal hyperplasia and increased sialomucin secretion in Crohn's disease, quiescent and active ulcerative colitis. In colitis with carcinoma inflamed mucosa away from the tumour had increased sialomucins as had colitis with dysplasia. They did not differ statistically from each other or from colitic controls without cancer. Dysplastic crypts frequently secreted sulphomucins and the increased sialomucins were in transitional-like glands in the surface fronds or adjacent to the dysplasia. A comparative study of the HID-AB technique gave total correct qualitative allocation of individual quantitatively assessed crypts. Routine HID-AB staining did not aid the recognition of dysplasia in ulcerative colitis. With the PB-KOH-PAS technique colorectal adenocarcinoma showed a significant diminution in O-acylated sialomucins compared with its adjacent mucosa. Mucosal dysplasia in ulcerative colitis displayed a similar trend in O-acylated sialic acid variants, differing with respect to age-and sex-matched colitic controls. The PB-KOH-PAS technique may be of help in assessing mucin secretion in ulcerative colitis as a guide to the evolution of malignancy.

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Section: Discussionmentioning

confidence: 99%

Mucin profiles in ulcerative colitis with dysplasia and carcinoma

1988

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“…4 Cool to 4'C and oxidise with precooled (4'C) 0 4 mM periodic acid in about 1 M hydrochloric acid (concentrated hydrochloric acid 1 volume, distilled water 4 volumes) at 4'C for one hour at room temperature (PA*) to oxidise completely all sialic acids.78 5 Wash in running water for 10 minutes. 6 Treat with 0-1% (w/v) sodium borohydride in 1% Gilks, Reid, Clement, Owen (w/v) dibasic sodium phosphate (anhydrous) for 20 minutes at room temperature to reduce aidehydes to Schiff unreactive primary alcohols. '2 7 Wash in running water for 10 minutes at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Simple procedure for assessing relative quantities of neutral and acidic sugars in mucin glycoproteins: its use in assessing cyclical changes in cervical mucins.

Gilks

Reid

Clement

et al. 1988

Journal of Clinical Pathology

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SUMMARy A simple histochemical procedure for assessing relative amounts of neutral and acidic sugars in mucin glycoproteins, and its application in the study of cyclical changes of human cervical mucins, is described. This procedure, the saponification/selective periodate oxidation/borohydride reduction/alcian blue pH 2-5/periodic acid Schiff (KOH/PA*/Bh/Ab 2-5/PAS) method, uses a selective oxidation step to remove the PAS positivity of sialic acid; thus only neutral sugars stain positively with PAS, and acidic sugars (0-sulphate esters and carboxyl groups) stain with alcian blue. This differs from the KOH/Ab/PAS technique which stains sialic acid residues with both alcian blue and PAS. Applying the KOH/PA*/Bh/Ab 2-5/PAS technique to the study of cyclical changes of human cervical mucins, a decreased neutral:acidic sugar ratio in the secretory phase mucins compared with those of the proliferative phase was found. This difference was not seen with KOH/ Ab/PAS staining in the same cases.The techniques and reagents used in this procedure can be easily applied in a clinical histopathology laboratory. In this paper we describe the use of PA* in the development of a histochemical technique, the saponification/selective periodate oxidation/borohydride reduction/Ab 2 5/PAS (KOH/PA*/Bh/Ab 2-5/PAS) technique, for the assessment of the relative quantities ofacidic sugars (0-sulphate esters, carboxyl groups) and "neutral sugars" with periodate oxidisable vicinal diols (hexoses, 6-deoxyhexoses, N-acetyl hexosamines) and its application to human cervical mucin. Material and methodsSpecimens of Sprague-Dawley rat liver, salivary glands (sublingual, submandibular, and parotid), and gastrointestinal tract, obtained fresh at necropsy, were fixed in 10% formalin-calcium for periods in excess of seven days. After fixation "swiss rolls" were prepared from the colon and the entire small intestine plus a strip of stomach.9 All the tissues were routinely processed in paraffin wax and embedded in Paraplast. Studies were also performed on formalin fixed, paraffin wax processed blocks of surgical specimens of

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“…Wahrend sich bei der HID-Reaktion Sialine mit Alcianblau darstellen lassen, werden diese bei Buk/Filipe mit der mPAS Reaktion nachgewiesen. Es wurde namlich mehrfach gezeigt, dal3 milde Periodatoxidation fast selektiv Sialinsauren mit Schiffscher Losung zur Reaktion bringt (Volz et al 1986). Die oben geschilderte KAP-Sequence verlauft zunachst wie die KOH-AB 1.0-mPAS Methode von Buk und Filipe (1986), d. h. nach Verseifung zur Freilegung veresterter OH-Gruppen werden bei niedrigem pH mit Alcianblau Sulfomuzine nachgewiesen, gefolgt von milder Periodatoxidation zur Darstellung der Sialomuzine.…”

Section: Diskussionunclassified

Zur pränatalen Entwicklung der Glandula mandibularis und Glandula parotis der Katze

Knospe¹,

Böhme²

1995

Anat Histol Embryol

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The prenatal development of the cat's mandibular and parotid gland was examined by means of serial section of 36 cat embryos at 14-62 days of development. Both glands were excised from the epithelium of the primitive oral cavity and branched up to day 36 of the branching phase into a specific connective tissue. This tissue contained besides fine collagenous fibres, a high amount of proteoglycans. In the subsequent separation phase, ducts and acini differentiated themselves in primitive lobules which were separated by connective tissue. In the prenatal differentiation phase, from about day 50 up to birth, intercalated ducts and striated ducts were formed. In the acini, mucous and serous cells contained different amounts of complex carbohydrates. This secretory component changed shortly before birth.

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Can ‘mild’ periodate oxidation be used for the specific histochemical identification of sialic acid residues?

Cited by 12 publications

References 15 publications

Mucin profiles in ulcerative colitis with dysplasia and carcinoma

Mucin profiles in ulcerative colitis with dysplasia and carcinoma

Simple procedure for assessing relative quantities of neutral and acidic sugars in mucin glycoproteins: its use in assessing cyclical changes in cervical mucins.

Zur pränatalen Entwicklung der Glandula mandibularis und Glandula parotis der Katze

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