Background: In the opinion of all field herpetologists and archaeozoologists, green frogs (living and sub-fossil specimens) are among the most difficult European amphibians to identify. Here, we examine the intra-and interspecific variation in both continuous and discrete iliac characters for refining the criteria for species-level identification among European green frogs Pelophylax ridibundus, Pelophylax lessonae and their associated klepton Pelophylax kl. P. esculentus (Amphibia, Ranidae). Results: A total of 454 ilia of modern green frogs from the former German Democratic Republic have been studied: 168 for P. lessonae (86 of males and 82 of females), 118 for P. ridibundus (44 of males and 74 of females) and 168 for P. kl. P. esculentus (86 of males and 82 of females). The origin, sex, population structure and phenotype are known for each of the studied specimens. Eight variables have been taken (one angle and seven measurements), mainly on the distal part of the element in order to be able to apply them to fragmentary fossil ilia. Interspecific variations, sexual dimorphism and population structure have been investigated. Results suggest that the secure determination of a single fossil ilium at species level is quite impossible, but that at population level, it may be possible to distinguish between a 'pure' species or a 'pure' species plus its cohabiting hybrid form, as some minor differences have been evidenced in particular in the angle of the tuber superior in relation to iliac's main axis (character 2) and the width of the pars ascendens (character 8); two parameters significantly non size-dependent. No sexual dimorphism has been detected, except for P. lessonae. Conclusions: We propose here a new biometrical method in order to differentiate between the fossil ilia of central European water frogs (genus Pelophylax) at population level.
The prenatal development of the cat's mandibular and parotid gland was examined by means of serial section of 36 cat embryos at 14-62 days of development. Both glands were excised from the epithelium of the primitive oral cavity and branched up to day 36 of the branching phase into a specific connective tissue. This tissue contained besides fine collagenous fibres, a high amount of proteoglycans. In the subsequent separation phase, ducts and acini differentiated themselves in primitive lobules which were separated by connective tissue. In the prenatal differentiation phase, from about day 50 up to birth, intercalated ducts and striated ducts were formed. In the acini, mucous and serous cells contained different amounts of complex carbohydrates. This secretory component changed shortly before birth.
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