Mucin profiles in ulcerative colitis with dysplasia and carcinoma

Allen, Derek C.; Connolly, N.; Biggart, J. D.

doi:10.1111/j.1365-2559.1988.tb02057.x

Cited by 24 publications

(21 citation statements)

References 49 publications

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“…distribution pattern in the intestinal epithelium have been demonstrated in various diseases that affect the colon [11][12][13] . Studies using biochemical, histochemical and immunohistochemical techniques have demonstrated that, in the different forms of colitis, abnormalities occur not only in the tissue content but also in the expression pattern of mucins along the crypts of the colon mucosa 21,22,[30][31][32][33] .…”

Section: Discussionmentioning

confidence: 99%

“…In has already been demonstrated that there is a notable increase in the tissue expression of sialomucins in the dysplastic colon mucosa of patients with ulcerative colitis 11 . Some authors consider that this change in the inflamed epithelium might precede the development of neoplasia, thus making it useful to investigate the tissue expression of mucins, in order to evaluate the risk of malignant transformation 12 . This evidence is corroborated by the results from experimental studies that demonstrated increased sialomucin production in colon mucosal cells in segments without fecal stream that were used to reconstruct the urinary transit 13 .…”

Section: Introductionmentioning

confidence: 99%

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Quantification by computerized morphometry of tissue levels of sulfomucins and sialomucins in diversion colitis in rats

et al. 2010

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PURPOSE: To quantify the intensity of sulfomucin and sialomucin expression in the colon mucosa, by means of computer-assisted image processing, comparing segments with and without fecal stream and correlating with the duration of fecal transit exclusion. METHODS: Forty-five Wistar rats were subjected to diversion of the fecal stream in the left colon by means of constructing a proximal colostomy and distal mucosal fistula. They were distributed randomly into three experimental groups of 15 animals, of which 10 were subjected to colon diversion (experimental subgroup) and five were only subjected to laparotomy, without colon diversion (control subgroup). The three experimental groups were formed according to the sacrifice date, which was to be performed six weeks after the surgical procedure (Group A), 12 weeks (Group B) and 18 weeks (Group C). The sulfomucin and sialomucin expression in the colon mucosa was evaluated using the histochemical technique of high iron diamine-alcian blue (HID-AB). The tissue expression was quantified for each animal, in the segments with and without fecal stream, at a location where there were four complete contiguous crypts in two random fields, with the aid of the computer-assisted image analysis software. The final value was taken to be the mean reading from the two fields selected, in the segments with and without fecal stream. To compare the expressions of the two mucin subtypes in the segments with and without fecal stream, the paired Student t test was used. To analyze variance according to duration of exclusion, ANOVA with the Newman-Keuls post-test was used, setting the significance level at 5% (p<0.05). RESULTS: There were significant reductions in tissue sulfomucin and sialomucin content in the colon without fecal stream, independent of the duration of exclusion considered. There was increased tissue sulfomucin content and decreased tissue sialomucin in the segments without fecal stream, with increasing duration of exclusion. CONCLUSIONS: Diversion of the fecal transit decreased the tissue sulfomucin and sialomucin content in the segments without fecal stream. Notwithstanding the reduction in the levels of both subtypes of acid mucin in the segments without fecal stream, there was increased tissue sulfomucin content and decreased tissue sialomucin with increasing duration of intestinal diversion.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantification by computerized morphometry of tissue levels of sulfomucins and sialomucins in diversion colitis in rats

et al. 2010

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“…The acid mucins, in turn, are composed of sulfomucins and sialomucins 7 . The proportions between neutral and acid mucins are constant and may be modified in IBD and changes to the tissue expression of this glycoprotein in the colon mucosa of patients with UC have already been demonstrated 7,8 . It has also been shown that such changes may even precede the development of neoplasia 7,8 .…”

Section: Introductionmentioning

confidence: 99%

“…The proportions between neutral and acid mucins are constant and may be modified in IBD and changes to the tissue expression of this glycoprotein in the colon mucosa of patients with UC have already been demonstrated 7,8 . It has also been shown that such changes may even precede the development of neoplasia 7,8 . Likewise, changes to mucin synthesis and distribution along the mucosa of colon segments without fecal stream that are used as reservoirs or by-pass for reconstituting the urinary transit have been demonstrated 9,10,11 .…”

Section: Introductionmentioning

confidence: 99%

Tissue quantification of neutral and acid mucins in the mucosa of the colon with and without fecal stream in rats

et al. 2009

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Purpose:To quantify the intensity of the expression of neutral and acids mucins in mucosa of the colon with and without fecal stream and to correlate this with the duration of fecal transit diversion. Methods: Thirty male Wistar rats were subjected to fecal transit deviation in the left colon by a proximal colostomy and a distal mucous fistula. The animals were divided into three experimental groups, according to whether sacrificing would be performed six, 12 or 18 weeks after surgery. The expression of neutral and acid mucins was evaluated using the histochemical techniques of Periodic Acid Schiff and Alcian Blue, respectively. The tissue mucins expression was quantified by computer-assisted image analysis software (NIS-Elements) in the segments with and without fecal stream. Student's paired t test was used to compare the quantities of mucins in colon with or without fecal stream and variance between the experimental groups by ANOVA and Newman-Keuls post-test, establishing level of signification of 5% (p<0.05). Results: There were significant decreased quantities of acid and neutral mucins in the colon without transit, compared with the colon with fecal stream, independent of the duration of exclusion. There was increased expression of neutral mucins in the colon with fecal stream after 12 and 18 weeks of exclusion. There was no increase in the expression of acid mucins in the colon with transit as the duration of fecal transit exclusion progressed. There was increased production of acid mucins in the animals submitted to diversion of the fecal stream for 18 weeks, compared with those subjected to diversion for 6 and 12 weeks. In the colon without fecal stream, there was increased expression of neutral mucins after 12 and 18 weeks of exclusion. Conclusions: Deviation of the fecal stream decreased the expression of acid and neutral mucins in the segments without fecal transit, compared with segments with transit. Regardless of the reduced expression of acid and neutral mucins in the segments without fecal stream, their tissue expression increased with increasing duration of intestinal deviation. Key words: Colitis. Colostomy. Mucins. Sialomucins. Image Processing, Computer-Assisted. RESUMOObjetivo: Quantificar a intensidade de expressão de mucinas neutras e ácidas na mucosa cólica provida e desprovida de trânsito intestinal relacionando-a ao tempo de exclusão fecal. Métodos: Trinta ratos Wistar machos foram submetidos à derivação do trânsito no cólon esquerdo por colostomia proximal e fístula mucosa distal. Os animais foram divididos em três grupos experimentais segundo o sacrifício ter sido realizado seis, 12 e 18 semanas após a cirurgia. A avaliação da expressão de mucinas neutras e ácidas na mucosa cólica foi realizada com as técnicas histoquímicas do Periódico Ácido de Schiff e Azul de Alcian, respectivamente. A quantificação da expressão tecidual das mucinas foi com auxílio de programa de análise de imagem assistida por computador (NIS-Elements) nos segmentos providos e desprovidos de trânsito fec...

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“…In active UC, the number of goblet cells, which synthesise the secretory mucins, are reduced and the mucus gel layer is thinner compared with mildly inflamed colon, independent of treatment.17 18 Concerning the 0-glycosylation of mucins, a reduction in mean number of sugar residues per oligosaccharide side chain has been reported,19 most probably resulting from premature termination of 0-glycosylation, leading to subsequent exposure of 'core-region' structures of the 0-linked oligosaccharides.5 20 Histochemically, a decrease in sulphomucins, with a reciprocal rise in sialomucins has been shown. [21][22][23] Some controversy still exists, however, regarding the staining techniques, because a comparative rise in either sialic acid or sulphate residues may change the balance of the staining pattern, independent of the exact number of these residues.22 24 Nevertheless, sialic acid residues seem to be increased, with loss of 0-acetyl substitutions.2426 As only 0-glycosylation was studied and not the underlying mucin gene expression, it was unclear, whether the abnormalities in 0-glycosylation result from changed post-translational processing or from changes in expression of different mucins. In fact, until recently, it was unknown which mucins were expressed in colon and whether the expression of these mucins was changed in UC.…”

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confidence: 99%