Can Formaldehyde Exposure Induce Histopathologic and Morphometric Changes on Rat Kidney?

Abstract

Formaldehyde is used traditionally for fixing the cadaver, and vaporized during dissection and practical studying on cadaver. This study was designed to determine the histopathologic and morphometric changes of rat kidney while all of the experiments were exposed to formaldehyde for 18 weeks. 28 male albino Wistar rats were divided into the following three experimental groups (E1: 2hrs/d, 2d/w; E2: 2hrs/d, 4d/w; E3: 4hrs/d, 4d/w) and one control group (C). when the exposure period was expired the animals were anaesthetized with chloroform. After cervical dislocation, the abdomen was dissected and the kidneys were taken. The kidney specimens were sectioned and stained with Haematoxylin and Eosin technique for histologic and morphometric study. Data were obtained from an Olympus light microscope and the analyzed with spss (version 11.5) and ANOVA test. In all histopathology sections of groups E1, E2 and E3, these similar changes were seen: mild glumerolar congestion, focal congestion, and vacuolar degeneration of tubular cells. There were no evidences of inflammatory cells infiltration or fibrotic changes of interstitial tissue. Only mild, non-specific congestion was seen in cortical vessels. Also there were not any abnormalities in the staining of nucleus and cytoplasm. According to Morphometric study, Mean

TBHP-induced oxidative stress alters microRNAs expression in mouse testis

“…In addition, germ cells disorganization and progressive degeneration accompanied by spermatogenesis failure were detected. These results are similar to pervious findings in which oxidative stress was induced by formaldehyde [32,51,18,30].…”

Sperm chromatin dispersion by formaldehyde in Wistar rats

“…With respect to our histopathologic analysis, the fact that no alterations in organs such as the liver, kidney, brain, and lungs were observed may be attributed to the short time of exposure to FA. In another study (Golalipour et al, 2009), no kidney damage was observed in rats exposed to FA vapors in cadaver-dissecting rooms. Regarding testicular damage induced by FA, some studies (Zhou et al, 2006;Vosoughi et al, 2012) reported similar results to those found in our study, while another report (Golalipour et al, 2007) described testicular damage after chronic exposure to FA vapors.…”

“…In another study (Golalipour et al, 2009), no kidney damage was observed in rats exposed to FA vapors in cadaver-dissecting rooms. Regarding testicular damage induced by FA, some studies (Zhou et al, 2006;Vosoughi et al, 2012) reported similar results to those found in our study, while another report (Golalipour et al, 2007) described testicular damage after chronic exposure to FA vapors. The morphologic changes described in the present study can be correlated with the alterations in reproductive parameters and sperm chromatin dispersion.…”

Effects of Formaldehyde and Vimentin Expression in Rat Skin

“…It is vaporised during the dissection and practical study of a cadaver. Previous studies have shown that this vapor may cause clinical symptoms such as throat, eye, skin and nasal irritation (Golalipour et al, 2007). Exposure to formaldehyde vapors can cause irritation of mucous membranes and allergic sensitization of skin, but the critical target is upper respiratory tract (Mathur & Rastogi, 2007) Vimentin filaments are more broadly distributed among tissues such as in the cells of the mesenchymal origin, certain other non-epithelial cells of various other tissues (Bavdek et al, 1997;Kacem et al, 1996;Lazarides, 1980;Rode et al, 1998) In this study, histopathological and immunohistochemical techniques due to the impact of the changes in formaldehyde and examined the distribution of vimentin.…”