Can embryo metabolism be used for selecting bovine embryos before transfer?

Donnay, Isabelle; Partridge, R.J.; Leese, Henry J.

doi:10.1051/rnd:19990501

Cited by 32 publications

(19 citation statements)

References 28 publications

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“…As 3-aminoisobutyrate can be derived from the TCA cycle intermediate succinyl-CoA, this may also be indicative of a decrease in TCA cycle metabolism. Previous studies have found a significant net appearance of alanine in the media of developing embryos [Houghton et al 2002] and it has been proposed that alanine provides a means of disposing of ammonia via the enzymes glutamate dehydrogenase and alanine transaminase [Donnay et al 1999]. A decrease in the levels of alanine in media has previously been associated with development to the blastocyst stage compared to those embryos that arrested [Houghton et al 2002].…”

Section: Discussionmentioning

confidence: 99%

¹H NMR based metabolic profiling of day 2 spent embryo media correlates with implantation potential

Wallace¹,

Cottell²,

Cullinane³

et al. 2013

Systems Biology in Reproductive Medicine

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Morphological assessment is currently the primary technique for selection of viable embryos for uterine transfer during assisted reproductive techniques, however this method has limited predictive power. The objective of this study was to employ NMR based metabolic profiling analysis of spent embryo culture media to identify novel biomarkers of embryo viability and provide insight into the metabolism of a viable embryo. A total of 37 patients undergoing IVF/ICSI treatment were recruited and 58 media samples were collected from embryos that were transferred back to the uterus. 1 H NMR spectra were acquired and analyzed resulting in the quantification of 12 metabolites in the media samples. Analysis of metabolite ratios revealed significant differences between those patients with positive (n ¼ 27) and negative (n ¼ 31) urinary bhCG results. Some of the most biologically relevant differences include a 17% increase in the formate to glycine ratio and a 22% decrease in the citrate to alanine ratio in the spent embryo media from the positive pregnancy group. Overall, the results indicate that metabolic profiling may provide a means of identifying biomarkers that aid selection of viable embryos.

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Section: Discussionmentioning

confidence: 99%

¹H NMR based metabolic profiling of day 2 spent embryo media correlates with implantation potential

Wallace¹,

Cottell²,

Cullinane³

et al. 2013

Systems Biology in Reproductive Medicine

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“…Embryos become more dependent on aerobic metabolism and probably acquire a greater capacity to use glucose as the blastocyst forms (48)(49)(50) *Significantly different from anticipated 1:1 ratio of males to females (P Ͻ 0.05). † Significantly different from anticipated 1:1 ratio of males to females (P Ͻ 0.01).…”

Section: Discussionmentioning

confidence: 99%

Sexual dimorphism among bovine embryos in their ability to make the transition to expanded blastocyst and in the expression of the signaling molecule IFN-τ

Larson

Kimura

Kubisch

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

185

138

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IFN-is a secretory product of trophectoderm of cattle, sheep, and their relatives and is expressed for a few days in early pregnancy after the blastocyst first forms. It serves to alert the mother that she is pregnant. A delayed or less than robust IFN-signal is a likely cause of embryonic loss. Here we have determined whether blastocyst production of IFN-, which is encoded by a cluster of genes on chromosome 9, differs between the sexes in cattle, as assessed by culture of in vitro-derived embryos on two different media, one complex (tissue culture medium 199 supplemented with serum) with coculture support, the other relatively simple (synthetic oviductal fluid plus albumin). With both media, female blastocysts produced approximately double the amount of IFN-as males, regardless of such variables as oocyte batch, blastocyst quality, hatching, and length of time in culture. However, in either tissue culture medium 199, which contains 5.5 mM D-glucose, or in synthetic oviductal fluid, in the presence but not in the absence of added glucose, significantly fewer female than male embryos were able to progress from the morula͞early blastocyst stage to more advanced stages of development. It is possible that the differences between male and female embryos both in their production of IFN-and in their ability to progress in development in glucose-rich media are manifestations of phenomena that occur in vivo and provide plasticity in embryo selection during early pregnancy.embryo culture ͉ in vitro maturation-in vitro fertilization ͉ sexual dimorphism

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“…Indeed, in other species, including human and mouse, embryos compensate for the absence of glucose by increasing pyruvate consumption or maintaining it at a high level (Gardner & Leese, 1988;Conaghan et al, 1993). However, pyruvate plays also other important roles in embryo culture (Donnay et al, 1999) that could mask such effects in our study.…”

Section: Discussionmentioning

confidence: 77%

“…The spent culture drops were frozen at -80 °C in the culture dishes. Pyruvate, glucose and lactate concentrations were evaluated microfluorimetrically (Leese & Barton, 1984;Donnay et al, 1999). Pyruvate and glucose uptakes and lactate release by the embryos were evaluated by comparing the concentrations of substrates in spent medium with those of control drops.…”

Section: Experiments 3: Pyruvate and Glucose Uptake And Lactate Releasementioning

confidence: 99%

Impact of adding 5.5 mM glucose to SOF medium on the development, metabolism and quality of in vitro produced bovine embryos from the morula to the blastocyst stage

Donnay

Feugang

Bernard

et al. 2002

Zygote

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Although toxic for early stages of embryo development, glucose is a physiological metabolic substrate at the morula and blastocyst stages. We evaluated the effect of adding 5.5 mM glucose from the morula stage on bovine blastocyst development and quality. In vitro matured and fertilised bovine oocytes were cultured in modified Synthetic Oviduct Fluid medium containing 5% fetal calf serum, but without added glucose, up to day 5 post-insemination (pi). Morulae were selected and further cultured in the presence or absence of 5.5 mM glucose. Blastocyst and hatched blastocyst rates were recorded. Oxygen, glucose and pyruvate uptakes as well as lactate release were evaluated. The quality of the resulting blastocysts was evaluated by the cell allocation to the inner cell mass (ICM) and trophectoderm (TE) and by the apoptotic index. Adding glucose increased the blastocyst rate at day 8 pi (80% vs 65%) but had no impact on hatching rate (25% vs 28%). A 22% decrease in oxygen uptake was observed in the presence of glucose, concomitant with an increase in lactate release, although no change was observed in pyruvate uptake. A slight decrease in blastocyst cell number was observed at day 7 in the presence of glucose while neither the ICM/TE cell ratio nor the apoptotic index were affected. In conclusion, adding 5.5 mM glucose from the morula stage has a limited impact on blastocyst rate and quality although important modifications were observed in embryo metabolism. It remains to be determined whether those modifications could influence embryo viability after transfer.

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Can embryo metabolism be used for selecting bovine embryos before transfer?

Abstract: -The quality of in vitro-produced bovine embryos remains variable.

Cited by 32 publications

References 28 publications

¹H NMR based metabolic profiling of day 2 spent embryo media correlates with implantation potential

¹H NMR based metabolic profiling of day 2 spent embryo media correlates with implantation potential

Sexual dimorphism among bovine embryos in their ability to make the transition to expanded blastocyst and in the expression of the signaling molecule IFN-τ

Impact of adding 5.5 mM glucose to SOF medium on the development, metabolism and quality of in vitro produced bovine embryos from the morula to the blastocyst stage

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Can embryo metabolism be used for selecting bovine embryos before transfer?

Abstract: -The quality of in vitro-produced bovine embryos remains variable.

Cited by 32 publications

References 28 publications

1H NMR based metabolic profiling of day 2 spent embryo media correlates with implantation potential

1H NMR based metabolic profiling of day 2 spent embryo media correlates with implantation potential

Sexual dimorphism among bovine embryos in their ability to make the transition to expanded blastocyst and in the expression of the signaling molecule IFN-τ

Impact of adding 5.5 mM glucose to SOF medium on the development, metabolism and quality of in vitro produced bovine embryos from the morula to the blastocyst stage

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¹H NMR based metabolic profiling of day 2 spent embryo media correlates with implantation potential

¹H NMR based metabolic profiling of day 2 spent embryo media correlates with implantation potential