Impact of adding 5.5 mM glucose to SOF medium on the development, metabolism and quality of <i>in vitro</i> produced bovine embryos from the morula to the blastocyst stage

Donnay, Isabelle; Feugang, Jean M.; Bernard, Sabine; Marchandise, Joëlle; Pampfer, S.; Moens, André; Dessy, F.

doi:10.1017/s0967199402002253

Cited by 18 publications

(8 citation statements)

References 70 publications

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“…Hardy et al described the measurement of pyruvate uptake before embryo transfer as a valuable new criterion for the selection of viable embryos that reached blastocyst stage. They observed that the pyruvate uptake in arrested embryos was significantly lower than for those embryos which developed to the blastocyst stage (66,67). However, to date whether the human embryo OC varies during early preimplantation development has been unclear.…”

Section: Discussionmentioning

confidence: 99%

Time-dependent O2 consumption patterns determined optimal time ranges for selecting viable human embryos

Tejera

Herrero

Viloria

et al. 2012

Fertility and Sterility

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Section: Discussionmentioning

confidence: 99%

Time-dependent O2 consumption patterns determined optimal time ranges for selecting viable human embryos

Tejera

Herrero

Viloria

et al. 2012

Fertility and Sterility

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“…Starting at the 8-16 cell stage, glucose metabolism rapidly increases as a result of the development of the glycolytic pathway [13]. Although adding 1.5 mM glucose after 48 h postfertilization has been shown to have a beneficial effect on embryo development [18], adding 5.5 mM glucose from the morula stage had only a small effect on the rate of blastulation and quality [14]. In this study, when the glucose concentration was increased from 1.5 to 3 mM at the morula stage, the rate of blastulation at day 7 after fertilization was lower than that obtained with 1.5 mM glucose.…”

Section: Discussionmentioning

confidence: 99%

Stage-Specific Effect of Growth Hormone on Developmental Competence of Bovine Embryos Produced In-Vitro

Iwata¹,

Ohota²,

Hashimoto

et al. 2003

Journal of Reproduction and Development

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Abstract. Many efforts have been made to develop effective culture conditions for the production of bovine blastocysts. Growth hormone (GH) and glucose are known to affect in vitro embryo development. To improve in vitro culture conditions, the culture medium containing fetal calf serum (FCS) or bovine serum albumin (BSA) was supplemented with GH at various periods of development, and the effects of GH on the rate of development and the quality of the blastocysts were studied. Then, starting at the morula stage, the effect of glucose and GH on the rate of development was studied. In all experimental periods, FCS was more effective than BSA at improving the development rate and increasing the cell number of blastocysts. Adding GH to the culture medium between 18 and 48 h after fertilization (1-8 cell stage embryo) did not affect either the rate of blastulation or the cell number regardless of the serum protein (FCS or BSA). From 48 to 120 h after fertilization (5-cell to morula stage) GH increased the cell number of the blastocysts in the presence of BSA, but not in the presence of FCS. From 120 to 192 h after fertilization (morula to blastocyst stage), GH improved the developmental rate and cell number in the presence of FCS, although there was no significant difference when BSA was used instead of FCS as the serum protein. When cows were implanted with blastocysts developed in the presence of GH from the morula stage, their pregnancy rate did not differ from that of the control. Increasing the glucose concentration in the medium from 1.5 mM to 3 mM starting at the morula stage (120 h after fertilization) slightly decreased the rate of development, but on the other hand, decreasing the glucose concentration to 0 mM did not affect either the rate of development or the cell number. Also, then GH had no effect on the developmental rate or the cell number in the absence of glucose. In conclusion, when the medium was supplemented with serum, GH improved embryo development from the morula stage, but an increased concentration of glucose decreased embryo development. Furthermore, GH did not improve the pregnancy rate of blastocysts developed in vitro.

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“…In vitro production of embryos Embryos were produced as previously described by Donnay et al (2002). Briefly, immature cumulusoocyte complexes (COCs) were obtained by aspirating follicles of from 2 to 8 mm from the ovaries of slaughtered cows.…”

Section: General Proceduresmentioning

confidence: 99%

Effect of prooxidant agents added at the morula/blastocyst stage on bovine embryo development, cell death and glutathione content

Feugang

Langendonckt²,

Sayoud

et al. 2003

Zygote

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Two prooxidant agents, 2,2′-azobis(2-amidinopropane)dihydrochloride (AAPH), a generator of free radicals in the culture medium, and buthionine sulfoximine (BSO), an inhibitor of glutathione synthesis, were used to reinforce from the morula stage (day 5 post-insemination, p.i.) the oxidative stress encountered by bovine embryos in culture. Exposure to increasing concentrations of both prooxidants from the morula stage did not affect blastocyst formation but some blastocysts were found degenerated on day 8 in a dose-dependent manner (0, 0.001, 0.01, 0.1 mM AAPH gave respectively 0, 10%, 32%, 48% degeneration, while 0, 0.1, 0.2, 0.4 mM BSO led respectively to 0, 14%, 30%, 41% degeneration). Hatching rates and cell numbers of surviving blastocysts were not affected. Morulae and early blastocysts exposed from day 5 to day 6 p.i. appeared more resistant than expanded blastocysts (75-80% survival vs 20-65%; p <0.05). Treatment with BSO significantly decreased the level of reduced glutathione in day 7 blastocysts (0.02 vs 0.42 pmol per embryo in the control) while AAPH had no effect (0.38 pmol per embryo). The proportion of cells showing membrane lesions was increased in degenerated blastocysts from day 7.5 p.i. In AAPH-treated, but not in BSO-treated embryos, cell membrane permeabilisation seems to occur before blastocyst degeneration. DNA fragmentation evaluated by the TUNEL technique was increased in day 7 blastocysts by both prooxidants (2.8 ± 0.4 in the control group vs 4.5 ± 0.4 and 6.0 ± 0.4 respectively in the AAPH- and BSO-treated groups). Addition of an inhibitor of caspase-3, DEVD-CHO, partially prevented DNA fragmentation, indicating that prooxidant treatment induced a caspase-dependent pathway of apoptosis.

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Impact of adding 5.5 mM glucose to SOF medium on the development, metabolism and quality of in vitro produced bovine embryos from the morula to the blastocyst stage

Cited by 18 publications

References 70 publications

Time-dependent O2 consumption patterns determined optimal time ranges for selecting viable human embryos

Time-dependent O2 consumption patterns determined optimal time ranges for selecting viable human embryos

Stage-Specific Effect of Growth Hormone on Developmental Competence of Bovine Embryos Produced In-Vitro

Effect of prooxidant agents added at the morula/blastocyst stage on bovine embryo development, cell death and glutathione content

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