cAMP-Mediated Catabolite Repression and Electrochemical Potential-Dependent Production of an Extracellular Amylase in<i>Vibrio alginolyticus</i>

Kim, Un Ok; Hahm, Kyung‐Soo; Park, Yong Ha; Kim, Young Jae

doi:10.1271/bbb.63.288

Cited by 3 publications

(1 citation statement)

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“…Above the initial viscosity of 2.4 mPas growth of V. ruber caused a dramatic decrease in viscosity of the medium (i.e., from 29.4 to 13.2 mPas). Vibrios are known to produce polysaccharide degrading enzymes like cellulases and chitinases (Keyhani and Roseman, 1999; Kim et al, 1999; Gao et al, 2011), which may hydrolyze HEC and cause a decrease in viscosity. Concentration of reducing sugars, as an indicator of cellulolytic activity was measured (Miller, 1959), but did not change significantly ( p = 0.915) in M9 medium with 1% (w/V) HEC during the incubation with V. ruber .…”

Section: Resultsmentioning

confidence: 99%

Viscosity dictates metabolic activity of Vibrio ruber

Borić¹,

Danevčič²,

Stopar³

2012

Front. Microbio.

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Little is known about metabolic activity of bacteria, when viscosity of their environment changes. In this work, bacterial metabolic activity in media with viscosity ranging from 0.8 to 29.4 mPas was studied. Viscosities up to 2.4 mPas did not affect metabolic activity of Vibrio ruber. On the other hand, at 29.4 mPas respiration rate and total dehydrogenase activity increased 8 and 4-fold, respectively. The activity of glucose-6-phosphate dehydrogenase (GPD) increased up to 13-fold at higher viscosities. However, intensified metabolic activity did not result in faster growth rate. Increased viscosity delayed the onset as well as the duration of biosynthesis of prodigiosin. As an adaptation to viscous environment V. ruber increased metabolic flux through the pentose phosphate pathway and reduced synthesis of a secondary metabolite. In addition, V. ruber was able to modify the viscosity of its environment.

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