Calogênese in vitro de segmentos apicais caulinares e internodais em segurelha (Satureja hortensis L.).

Navroski, Márcio Carlos; Waldow, Daniel Arthur Gaklik; Pereira, Mariane de Oliveira; Pereira, Alessandra de Oliveira

doi:10.18227/1982-8470ragro.v6i3.698

Cited by 5 publications

(3 citation statements)

References 6 publications

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“…Similarly, Navroski et al (2012) observed that when using lower concentrations of BAP, callus formation was reduced in stem segments of Satureja hortensis L., and a concentration of 19.45 μM resulted in more callus formation. The effects of BAP and 2,4-D on Sorghum bicolor explants indicated that 4 mg L of 2,4-D combined with 1 mg L of BAP were more efficient to callus induction (Espinoza-Sánchez et al 2018).…”

Section: Mcr Araújo Et Almentioning

confidence: 75%

Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant

Araújo

Chagas

Vendrame

et al. 2021

Crop Breed. Appl. Biotechnol.

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Caçari is a fruit tree that is native to the Amazon region of Brazil. The fruit is considered to have the highest vitamin C content of any edible fruit (13.757 mg 100 g -1 dry weight). The objective of this study was to evaluate the effects of 2,4-D and 6-BAP alone or in combination on in vitro callus induction and pro-embryogenic mass formation in caçari stem segments in different culture media as a precursor for caçari micropropagation. The experiment consisted of two culture media (MS and WPM) and combinations of four concentrations of 2,4-D (0, 1, 2 and 4 mg L -1 ) and BAP (0, 0.25, 0.5 and 1 mg L -1 ). The results showed that the highest percentage of callus formation (99%) was obtained in WPM supplemented with 4 mg L -1 , 2,4-D and 1 mg L -1 BAP, and this combination resulted in 93% pro-embryogenic mass formation.

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Section: Mcr Araújo Et Almentioning

confidence: 75%

Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant

Araújo

Chagas

Vendrame

et al. 2021

Crop Breed. Appl. Biotechnol.

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“…According to Navroski et al (2012), the use of BAP and ANA in the culture medium promotes the increase in the formation of callus in Segurelha (Satureja hortensis L.).…”

Section: Resultsmentioning

confidence: 99%

Silicon in the Embriogenic Potential of Callus in vitro of Passiflora edulis

Dias¹,

Anjos²,

Souza³

et al. 2018

JAS

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Adding silicon to the culture medium may contribute, to improve many growth parameters including embryogenesis and organogenesis. The objective of this study was to evaluate the embryogenic potential of passion fruit in vitro (Passiflora edulis f. Flavicarpa O. Deg), submitted to different concentrations of silicon. Nodal segments of plants were inoculated at five concentrations of silicic acid added to the culture medium, 0.0; 0.5; 1.0; 1.5 and 2.0 g L -1. After 60 days, the visual characteristics (% contamination, callus, bud, root and oxidation), cytochemical test and microanalysis of X-rays evaluated. Concentrations of 0.5; 1.0 and 2.0 g L -1 of silicon did not differ in relation to callus formation. By the cytochemical test, all treatments presented embryogenic cells indicated by the reaction to acetic Carmine and, little reaction to the Evans blue associated with cell death. By the microanalysis of X-rays, the nutrients sulfur and phosphorus, presented significant absorption responses at the concentration of 1.5 g L -1 of silicon in comparison to the other concentrations. The silicon added to the culture medium has a response in the formation of cells with embryogenic potential in nodal segments of passion fruit. The concentration of 2.0 g L -1 of silicon acid (H 4 SiO 4 ) has a high embryogenic potential in passionflower cells, Passiflora edulis f. Flavicarpa O. Deg.

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“…Callus consistency is influenced by factors such as auxin, cytokinin, explant type and genotype (Navroski et al, 2012).…”

Section: Histologymentioning

confidence: 99%

Induction, Growth Kinetics and Morpho-histological Characterization of Neem Callus

Oliveira¹,

Oliveira²,

Machado³

et al. 2018

JAS

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Azadirachta indica A. Juss, popularly known as neem, is a species native to India, belonging to family Meliaceae, considered the most important plant species with insecticidal action. The aim of this study was to evaluate the influence of growth regulators on induction and growth of neem callus and to observe their viability for embryogenesis through morpho-histological characteristics. In vitro germinated plants were used for excision of nodal explants. These segments were inoculated in Murashige and Skoog culture medium containing 1.0 mg/l 2,4-D (2,4-dichlorophenoxyacetic) combined with BAP (6-benzylaminopurine) at the following concentrations: 0.0, 0.5, 1.0 and 2.0 mg/l (T1, T2, T3 and T4 respectively), for callus induction. At 0 (mass of nodal segments without callus), 20, 40 and 60 days of culture, the percentage of callus formation was observed and the callus weight was measured for each treatment and at the end of the 60 days, consistency, color, and cell histology were evaluated. There was callus formation in all treatments tested. The highest induction of Azadirachta indica A. Juss callus is observed in the presence of 1.0 mg/l 2,4-D + 2.0 mg/l BAP, with callus showing light brown color, friable consistency and rounded cells with intense cell division, typical of cells with potential embryogenic capacity.

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Calogênese in vitro de segmentos apicais caulinares e internodais em segurelha (Satureja hortensis L.).

Cited by 5 publications

References 6 publications

Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant

Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant

Silicon in the Embriogenic Potential of Callus in vitro of Passiflora edulis

Induction, Growth Kinetics and Morpho-histological Characterization of Neem Callus

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