Callus Induction and Plant Regeneration from Different Explants of Actinidia deliciosa

Akbaş, Filiz; Işıkalan, Çiğdem; Namli, Sureyya

doi:10.1007/s12010-008-8401-2

Cited by 22 publications

(12 citation statements)

References 15 publications

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“…Also regression analyses indicated that higher concentrations of BAP had an inhibitory effect on proliferation. Our results are in agreement with those of Akbas et al [39] on Amygdalus communis L. that the decrease of proliferation rate, length of micro-shoot and an increase of callus end of micro-shoot are due to the strong concentrations of BAP which inhibit them.…”

Section: Discussionsupporting

confidence: 94%

Effects of nutrient media, different cytokinin types and their concentrations on in vitro multiplication of G×N15 (hybrid of almond×peach) vegetative rootstock

Arab

Yadollahi

Shojaeiyan

et al. 2014

Journal of Genetic Engineering and Biotechnology

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The objective of this research was to assess the effects of different media i.e. Murashige and Skoog (MS) and Quoirin and Lepoivre (QL), cytokinin type i.e. 6-Benzyladenin (BA) and 6-Benzylaminopurine (BAP) and cytokinin concentration on in vitro proliferation of the G · N15 rootstock. To evaluate the effects of different media and cytokinin type, two separate experiments were conducted as factorial based on completely randomized design, and single nodes were used as explants. The results showed that MS nutrient medium was found to be superior to QL nutrient medium. Regarding the interaction between media and growth regulators, the best interaction was found in MS medium supplemented with 1 mg l À1 BAP resulting in 8.5 new micro shoots/explant while 7.75 shoots were observed in MS medium containing 1.25 mg l À1 BA. The longest length of new microshoots (2.10 cm) was obtained in hormone-free MS medium. Findings of this study showed that there is a significant correlation between the hormone level and plantlet height and formed callus weight so that an increase in BAP and BA levels in both of MS and QL media resulted significantly in height decrease and callus weight increase. The results also suggest that the best and the worst plantlets in terms of quality were observed in hormone-free QL medium and MS medium supplemented with 1.25 mg l À1 , respectively. These results reflect the fact that the presence of high amounts of NH 4 NO 3 and cytokinin especially BAP in culture medium triggered inhibitory effect on shoot growth. ª 2014 Production and hosting by Elsevier B.V. on behalf

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Section: Discussionsupporting

confidence: 94%

Effects of nutrient media, different cytokinin types and their concentrations on in vitro multiplication of G×N15 (hybrid of almond×peach) vegetative rootstock

Arab

Yadollahi

Shojaeiyan

et al. 2014

Journal of Genetic Engineering and Biotechnology

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“…Furthermore, at day 45 of culture, a CG rate of 3.8-4.0 was detected for all explant types (Figure 3b) suggesting that callus production in Aster is species and genotype dependent (Cammareri et al 2001). In plant species such as Aster tripolium L. (Uno et al 2009), Saussurea obvallata (Dhar & Joshi 2005) and Actinidia deliciosa (Akbas et al 2009) profuse callus production and proliferation were obtained using leaf explants. On the contrary, for Changium smyrnioides, petioles were the best explants for obtaining callus culture (Jiang et al 2009).…”

Section: Resultsmentioning

confidence: 99%

Establishment ofAster sedifoliusandAster caucasicuscallus cultures as a potential source of antioxidants

Minutolo

Caruso

et al. 2012

Plant Biosystems - An International Journal Dealing with all As

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Callus cultures were established for Aster sedifolius and Aster caucasicus, two Aster species used in natural medicine for their anticancer, antibacterial and antiviral activities attributed to the high content of antioxidant compounds such as polyphenols and ascorbate. The effects of growth medium and light condition on the induction and growth rate of callus from leaf, petiole and root explants are reported. Callus induction and proliferation depended on the genotype and the experimental conditions. In particular, a profuse callus culture was obtained from leaf explants grown in the light on medium supplemented with 2,4-D (0.1 mg l 71 ) for A. caucasicus and on medium supplemented with 2,4-D (0.44 mg l 71 ) plus 6-benzil-ammino-purine (BAP) (0.22 mg l 71 ) for A. sedifolius. The content of total polyphenol and ascorbic acid was estimated in leaf and petiole explants of in vivo plants and in the relative derived calli. In calli, polyphenol content was lower than in the corresponding in vivo organs. Furthermore, the total ascorbic acid content decreased in calli while the reduced ascorbic acid pool increased. These findings demonstrate that Aster callus cultures produce antioxidant compounds and as such might be a model system to investigate the regulation and production of these important metabolites.

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“…Many PGRs have been used in tissue culture of Actinidia species, and their effects have varied depending on the species and explant type used (Mezzetti et al 1991a(Mezzetti et al , 1991bInfante et al 1994;Akbas et al 2009;Moncalean et al 2009). …”

Section: Discussionmentioning

confidence: 99%

In vitro organogenesis and plant regeneration from leaves ofActinidia erianthaBenth. cv White (kiwifruit)

Xie

Long

2011

New Zealand Journal of Crop and Horticultural Science

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Plantlets were regenerated from leaf explants of Actinidia eriantha Benth. cv White (kiwifruit). The best medium for adventitious shoot formation from leaf blade strips was Murashige-Skoog (MS) medium with 0.1 mg L(1 NAA and 2.0 mg L (1 BA. The frequency of shoot regeneration (%) was dependent on the size and the part of the leaf from which the explant was taken. The highest frequency of shoot regeneration was 7.3092.61 shoots per explant, for explants taken from within 0Á3 cm of the leaf blade base, near a vein and not on the leaf edge. When using petiole-derived explants, a maximum frequency of adventitious shoot regeneration of 79.17% with 6.5492.31 shoots per explant was obtained using 0.1 mg L(1 NAA and 2.0 mg L (1 ZT in MS medium. The highest rate of rooting obtained was 96.67% when using 1/2 MS medium containing 0.6 mg L(1 IBA and 93.75% when using wet moss without IBA. This is the first report to study the effect of explants taken from different parts of the leaf blade on shoot regeneration in A. eriantha. All plantlets were transferred to pots of peat/perlite and grown on before being planted in the field. Plantlets were successfully acclimatized to field conditions and produced healthy plants.

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Callus Induction and Plant Regeneration from Different Explants of Actinidia deliciosa

Cited by 22 publications

References 15 publications

Effects of nutrient media, different cytokinin types and their concentrations on in vitro multiplication of G×N15 (hybrid of almond×peach) vegetative rootstock

Effects of nutrient media, different cytokinin types and their concentrations on in vitro multiplication of G×N15 (hybrid of almond×peach) vegetative rootstock

Establishment ofAster sedifoliusandAster caucasicuscallus cultures as a potential source of antioxidants

In vitro organogenesis and plant regeneration from leaves ofActinidia erianthaBenth. cv White (kiwifruit)

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