Effects of nutrient media, different cytokinin types and their concentrations on in vitro multiplication of G×N15 (hybrid of almond×peach) vegetative rootstock

Arab, Mostafa; Yadollahi, Abbas; Shojaeiyan, Abdolali; Shokri, S.; Ghojah, Shoresh Maleki

doi:10.1016/j.jgeb.2014.10.001

Cited by 80 publications

(54 citation statements)

References 29 publications

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“…Plants grown on M2 medium showed the lowest shoot length (1.8 cm on average) although not statistically different from M3 medium (Table 1). For the G × N15 hybrid (almond tree × peach tree), it was similarly reported that the highest shoots length in vitro were achieved on culture media without plant growth regulators supplementation, which may be explained mainly by the cytokinin effects on boosting shoots emission, inducing plants to produce a higher number of nutrients sinks with lower individual growth and elongation (Arab et al, 2014). Additionally, cytokinins are known to reduce apical dominance and induce adventitious shoots formation from meristematic explants (Resmi & Nair, 2011).…”

Section: Culture Media and Plant Regenerationmentioning

confidence: 98%

Micropropagation and Establishment of Humulus lupulus L. Plantlets Under Field Conditions at Southern Brazil

Machado¹,

Gomes²,

Francisco³

et al. 2018

JAS

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Tissue culture technique can be an important approach for the rapid propagation of Humulus lupulus L. (hop plant). The present study evaluated different culture media for hop plants (cv. Columbus) in vitro shoots multiplication, as well as ex vitro rooting and plantlets establishment under field conditions at Southern Brazil. The culture medium containing the plant growth regulator thidiazuron (TDZ) resulted in greater in vitro shoots multiplications (five shoots per explant). For microcuttings ex vitro rooting, indole-3-butyric acid (IBA) application at a concentration of 3000 mg L -1 resulted in higher rooting and survival percentages during acclimatization. After 90 days of acclimatization, the plantlets were transplanted under field conditions and had their development and productivity evaluated 122 days after transplanting. Micropropagated plants achieved 100% survival under field conditions. Nitrogen supply is highly necessary for hop plants initial development at Southern Brazil. Micropropagation is a viable technique for quality plantlet production of H. lupulus cv. Columbus.

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Section: Culture Media and Plant Regenerationmentioning

confidence: 98%

Micropropagation and Establishment of Humulus lupulus L. Plantlets Under Field Conditions at Southern Brazil

Machado¹,

Gomes²,

Francisco³

et al. 2018

JAS

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“…Besides, this could also be attributed to variation in the physiological condition of the plant due to time/season of explant collection. Such difference has also been reported in other plant species [26]. Further, a combination of cytokinin and auxin (2.0 mg/l BAP + 0.5 mg/l NAA) was used by Srivastava and Srivastava [8] for micropropagation of P. foetida through nodal culture.…”

Section: Axillary Shoot Proliferation From Mature Nodal Explantmentioning

confidence: 68%

“…Thus, the effect of plant growth regulators is conflicting on axillary shoot proliferation in P. foetida. These types of results could be due to the difference in the genotype of the plant and/or age of the explant [26,27]. Besides, this could also be attributed to variation in the physiological condition of the plant due to time/season of explant collection.…”

Section: Axillary Shoot Proliferation From Mature Nodal Explantmentioning

confidence: 99%

In vitro propagation by axillary shoot proliferation, assessment of antioxidant activity, and genetic fidelity of micropropagated Paederia foetida L.

2018

J App Biol Biotech

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An efficient plant regeneration protocol through axillary shoot proliferation of nodal explants of a valuable medicinal plant, Paederia foetida, has been developed. Highest shoot multiplication was found on Murashige and Skoog's (MS) medium supplemented with 3.0 mg/l N 6 -benzylaminopurine. Upscaling of shoots was accomplished from axenic nodal explants derived from primary in vitro regenerated shoots on fresh medium of the same composition. Thus, we could obtain 71-76 shoots, in vitro, starting from a single node within 11-12 weeks. These shoots were successfully rooted on ½ MS medium and acclimatized in soil. The biochemical fidelity was assessed by evaluating the antioxidant activities of leaves of both field grown mother plant and micropropagated plants. The antioxidant activities, as carried out by 2, 2-diphenyl-1-picrylhydrazyl and metal chelating assay, of leaves collected from micropropagated plants were at par with activity of leaves collected from mother plant. Most importantly, both sources have significant antioxidant activities compared to the standard, butylated hydroxytoluene. Further, genetic fidelity of the micropropagated plants with that of the mother plant was assessed by inter simple sequence repeats markers, and their true-to-type nature was confirmed on the basis of monomorphic banding profile. This protocol has potential to create large number of clonal plants with similar antioxidant activities as the natural plants and thus could be useful for drug research and development.

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“…These magnitudes significantly differed from each other (Tukey, 0.05). This effect could be attributed to BAP, which induces cell division of somatic cells and synthesis of specific proteins (Arab et al, 2014) that lead to later formation of meristematic structures that develop in the shoots. However, the quantity of BAP is related to how much meristematic structure can be induced (Siddique and Anis, 2009).…”

Section: Resultsmentioning

confidence: 99%

Benzylaminopurine and indol-3-acetic acid concentrations in in vitro proliferation of Agave angustifolia adventitious shoots

Ríos-Ramírez

Valle

Rodríguez-Ortiz

et al. 2017

Ciencia e investigación agraria

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S. Ríos-Ramírez, J. Enríquez-del Valle, G. Rodríguez-Ortíz, and J. Ruíz-Luna. 2017. Benzylaminopurine and indol-3-acetic acid concentrations in in vitro proliferation of Agave angustifolia adventitious shoots. Cien. Inv. Agr. 44(3): 285-294. Today, a micropropagation method for Agave angustifolia exists, but for the multiplication of propagules, more information is needed on the diverse components of the culture medium, cytokinins and auxins and their effect on organogenetic response. The objective of this study was to evaluate dosages of benzylaminopurine and indol-3-acetic acid (IAA) in a culture medium and their effect on the formation of new adventitious shoots (organogenesis). The explants used were clusters of shoots on a common base of A. angustifolia stem tissue. Clusters of three to four shoots were established in different variants of a Murashige and Skoog culture medium that had different concentrations of benzylaminopurine (BAP; 0.5, 1, 2, and 4 mg L -1 ) and IAA) (0.1, 0.3, and 1 mg L -1 ). After 16 wk, it was found that a higher proliferation of shoots was positively related to the concentration of growth regulators. Explants formed a greater quantity of shoots in a culture medium with an increase in BAP of up to 4 mg L -1 and in IAA of up to 1 mg L -1 , and there was a synergistic effect with BAP to induce the formation of the greatest number of shoots.

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Effects of nutrient media, different cytokinin types and their concentrations on in vitro multiplication of G×N15 (hybrid of almond×peach) vegetative rootstock

Cited by 80 publications

References 29 publications

Micropropagation and Establishment of Humulus lupulus L. Plantlets Under Field Conditions at Southern Brazil

Micropropagation and Establishment of Humulus lupulus L. Plantlets Under Field Conditions at Southern Brazil

In vitro propagation by axillary shoot proliferation, assessment of antioxidant activity, and genetic fidelity of micropropagated Paederia foetida L.

Benzylaminopurine and indol-3-acetic acid concentrations in in vitro proliferation of Agave angustifolia adventitious shoots

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