Callus induction and plant regeneration of Michelia champaca (Magnoliaceae): A multipurpose tree

Abstract

Michelia champaca callus was induced from axillary bud explants cultured onto Murashige and Skoog (MS) basal medium supplemented with different concentrations of auxins (IAA and 2,4-D) and cytokinin (BAP) as plant growth regulators. It was observed that callus of M. champaca could be induced on MS media supplemented with 0.0-2.0 mg/l IAA, 0.0-2.0 mg/l 2,4-D, and 0.0-2.0 mg/l BAP, respectively. All growth regulators that were used in this study are capable of inducing callus from explants. 0.10 mg/l IAA and 0.10 mg/l 2,4-D were found to be the suitable concentrations for the induction of callus. The survival rate of explants in the media supplemented with IAA was found to be 81%. MS Media supplemented with different concentrations of BAP combined with IAA and 2,4-D were tested for callus proliferation. The callus growth showed good response to all growth regulators combinations tested in this study, except the combination BAP/IAA with 0.2/0.10 mg/I which exhibited low response with dark brown callus. The combination 2.00 mg/l BAP with 0.50 mg/l IAA and all the combinations of BAP with 2,4-D showed the highest rate of callus proliferation with cream colour. This study suggests that auxins along with cytokinins have a critical role on the callus and shoot proliferation of this plant.

Shoot Multiplication and Callus Induction of Labisia pumila var. alata as Influenced by Different Plant Growth Regulators Treatments and Its Polyphenolic Activities Compared with the Wild Plant

“…Furthermore, 2,4-D is the most significant PGR used for the proliferation of callus, callus induction, and also callus maintenance, according to Rao et al [ 44 ]. Michelia champaca (Magnoliaceae) Linn callus may also be induced on MS medium supplemented with 2,4-D at a concentration of 2 mg/L or lower, according to Abdelmaged et al [ 45 ]. A 100% callus induction was observed in the current study when a combination of 0.5 mg/L 2,4-D and 0.25 mg/L zeatin was used.…”

PERKEMBANGAN KULTUR DAUN Aglaonema sp. var Siam Pearl, Aglaonema sp. var. Lady Valentin dan Aglaonema sp. var. Lipstik DENGAN PERLAKUAN ZAT PENGATUR TUMBUH IAA DAN BAP

“…Lama waktu yang dibutuhkan untuk induksi kalus cukup lama dibandingkan dengan induksi kalus pada tanaman lain. Kalus mulai muncul minggu ke-2 masa kultur pada tanaman Sonchus arvensis, L. (Wahyuni et al, 2010), Cicer arietinum L. (Huda et al, 2003), Solanum nigrum L. (Sridar and Naidu, 2011), dan Michelia champaca (Abdelmageed et al, 2012), minggu ke-3 masa kultur pada Ananas comosus L. (Merr.)…”

“…(Bai and Qu, 2000), 7 kultivar Cucumis melo L dengan perlakuan kombinasi konsentrasi 0-0,5 mg/l IAA + 0,1-1 mg/l BAP (Melara et al, 2009), 5 kultivar padi Jawa (Rachmawati and Anzai, 2006), 11 kultivar Melissa officinalis L. Dengan perlakuan IAA+BAP (Mohebalipour et al, 2012) dan 5 kultivar Triticum aestivum dengan perlakuan 2,4-D+Kinetin (Rashid et al, 2009) menunjukkan prosentase keberhasilan induksi kalus yang berbeda, baik dalam inisiasi maupun pertumbuhannya. Selanjutnya induksi kalus dengan perlakuan IAA + BAP juga berhasil pada tanaman Solanum nigrum L. (Sridar and Naidur, 2011), dan Michelia champaca (Abdelmageed et al, 2012).…”

Biotechnological Approaches for the Improvement of Magnolia Genus Grown in Indonesia

“…Previous studies have been conducted to investigate volatile compounds of M. alba [3,5,10,11] and chemical composition and bioactive constituents of M. champaca [12,13]. Other, a few studies on the tissue culture report plant regeneration on some species of Michelia, [14], especially callus induction, cell culture, somatic embryogenesis and dormancy of M. champaca [15,16,17,18] and cell culture of M. alba [19]. There are a few tissue culture studies on M. alba, M. liliifera and Magnolia that grow in Indonesia.…”

“…The various explants and plant growth regulators were used to get appropriate protocol for callus and shoot initiation of M. champaca. The auxillary bud and petiole explants responsed to both single auxin and cytokinin on callus induction of M. champaca[17,52]. Although all of hormones inducted callus, the best callus formation is noted on 2,4-D, single exogenous auxin with different medium.…”