Callus Induction and Bioactive Compounds from<i>Piper betle</i>L. var nigra

Junairiah,; Mahmuda, A.; Manuhara, Yosephine Sri Wulan; Ni’matuzahroh,; Sulistyorini, Lilis

doi:10.1088/1755-1315/217/1/012026

Cited by 9 publications

(7 citation statements)

References 7 publications

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“…Plant growth regulators are essential for the growth and production of secondary metabolites from plant cultures. Benzyl amino purine and naphthalene acetic acid combination was found essential for callus production from the roots and leaves of Atropa acuminate. Thidiazuron was found to be superior to both kinetin and benzyl adenine in the growth and production of photosynthetic pigments in organ cultures of Hyssopus officninalis. The use of a combination of plant growth regulators such as kinetin and benzyl amino purine resulted in a marked effect on callus growth and the type of accumulated metabolites . In the current study, the use of kinetin in two concentrations showed a marked difference in the accumulation of both rutin and quercetin in cultured shoots.…”

Section: Discussionmentioning

confidence: 96%

Effect of Light Quality and Media Components on Shoot Growth, Rutin, and Quercetin Production from Common Buckwheat

Gabr

Fayek

Mahmoud³

et al. 2022

ACS Omega

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Common buckwheat ( Fagopyrum esculentum Moench) seeds are important nutritious grains that are widely spread in several human food products and livestock feed. Their health benefits are mainly due to their bioactive phenolic compounds, especially rutin and quercetin, which have a positive impact on heart health, weight loss, and diabetes management. In this study, we evaluated different media and light treatments for the in vitro cultures of common buckwheat (CB) in order to find the most optimum one producing the highest yield with the highest purity of these compounds. The subcultured treated samples included in this study were shoots, leaves, stems, hairy roots, and calli. From the several treated samples and under different light stress conditions, the best production was achieved by growing the shoots of common buckwheat in hormone-free media containing activated charcoal and exposing to blue light, attaining 4.3 mg and 7.0 mg/g of extracts of rutin and quercetin, respectively, compared to 3.7 mg of rutin/g of extract and traces of quercetin in the seeds of CB. Continuous multiplication of CB shoots in the media containing charcoal and different concentrations of kinetin produced an extract with 161 mg/g of rutin and 26 mg/g of quercetin with an almost 20-fold increase in rutin content. The rutin content under these conditions reached up to 16% w/w of the extract. The hairy root cultures of the leaves exposed to red light showed a significantly high yield of quercetin attaining 10 mg/g of extract. Large-scale production of CB shootlets under the best conditions were carried out, which enabled the isolation of pure quercetin and rutin using a simple chromatographic procedure. The identity and purity of the isolated compounds were confirmed through NMR and HPLC analyses.

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Section: Discussionmentioning

confidence: 96%

Effect of Light Quality and Media Components on Shoot Growth, Rutin, and Quercetin Production from Common Buckwheat

Gabr

Fayek

Mahmoud³

et al. 2022

ACS Omega

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“…The callus that form is generally yellowish-white in colour and compact in texture. More secondary metabolites can accumulate in a compact callus texture [20].…”

Section: Callus Morphologymentioning

confidence: 99%

Callus induction of Calotropis gigantea using BAP and 2,4-D in vitro

Muthiah

Sakya

Setyawati

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Calotropis gigantea is a plant not widely used but has potential as a medicinal plant. The phytochemical compound can be produced faster using a tissue culture and a combination of growth regulators. The research objectives were to study the effect and determine the best concentration of BAP and 2,4-D in callus induction of Calotropis gigantea. This research was carried out in the Plant Physiology and Biotechnology Laboratory, Faculty of Agriculture, Sebelas Maret University, from September to November 2021. A month-old Calotropis gigantea stem was used as an explant. The research used three replicates of a completely randomized design with 25 combination treatments. The culture medium was MS with BAP (0; 0.5; 1; 1.5; 2 ppm) and 2,4-D (0; 0.5; 1; 1.5; 2 ppm) supplements. Parameters observed in this research were callus formation time, callus colour, and callus texture. The results showed that a combination of BAP 0.5 ppm + 2,4-D 1.5 ppm could induce callus faster than other treatments, 6 days. Callus morphology with green color and compact texture resulted from a combination of BAP 1.5 ppm + 2,4-D 1.5 ppm treatment.

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“…A compact callus is a callus that has a dense cell arrangement, while a crumb callus is a callus that has a loose and fragile cell structure. According to [13], the Callus's brittle texture indicates that the callus will develop into a somatic embryo. 3, the texture of the callus formed was compact and crumbly.…”

Section: Callus Texturementioning

confidence: 99%

The Effect of NAA and BA on In Vitro Callus Induction of Biduri (Calotropis gigantea)

Wulandari,

Sakya,

Hartati

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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A family of medicinal plants known as biduri (Calotropis gigantea) has a number of useful traits. Alkaloids, glycosides, phenolics, tannins, flavonoids, terpenes, and sterols are just a few of the chemical substances found in biduri. One method used to boost the generation of secondary metabolites is biduri callus induction by tissue culture. The goal of the study was to find the ideal growth regulator concentration for callus biduri induction. The study employed a completely randomized design (CRD), which was carried out three times and was set up in a factorial fashion with two treatment components and 25 treatment combinations. The growth regulator NAA and the growth regulator BA were the first and second factors, respectively. ANOVA was used to examine the data with a 5% level test. The Duncan Multiple Range Test (DMRT) was conducted at a 5% level if there was a significant difference. A compact, green callus texture was generated by applying one ppm NAA and three ppm BA. At the same time, a callus on the biduri could not have been treated without an NAA application. Further research can be done by subculture callus biduri when the callus is still fresh and green to produce secondary metabolites.

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Callus Induction and Bioactive Compounds fromPiper betleL. var nigra

Cited by 9 publications

References 7 publications

Effect of Light Quality and Media Components on Shoot Growth, Rutin, and Quercetin Production from Common Buckwheat

Effect of Light Quality and Media Components on Shoot Growth, Rutin, and Quercetin Production from Common Buckwheat

Callus induction of Calotropis gigantea using BAP and 2,4-D in vitro

The Effect of NAA and BA on In Vitro Callus Induction of Biduri (Calotropis gigantea)

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