Calcium phosphate bone cement with 10wt% platelet-rich plasma in vitro and in vivo

Chen, Jian-Chih; Shih, Chi‐Jen; Tien, Yin-Chun; Chen, Wen‐Cheng

doi:10.1016/j.jdent.2011.11.003

Cited by 31 publications

(14 citation statements)

References 35 publications

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“…Moreover, the use of biomaterials based on bone cement, which contains calcium ions, seems to play an especially important role in bone regeneration. Calcium ions likely work as thrombocyte activators . Thus, the combination of PRP with biomaterials such as HA, beta tricalcium phosphate, or other ceramics and MSCs enrichment may enhance bone regeneration and improve cell proliferation and differentiation …”

Section: Discussionmentioning

confidence: 99%

“…Calcium ions likely work as thrombocyte activators. 50,51 Thus, the combination of PRP with biomaterials such as HA, beta tricalcium phosphate, or other ceramics and MSCs enrichment may enhance bone regeneration and improve cell proliferation and differentiation. 50,51 Scaffolds with drug delivery systems based on coaxial nanofibers with optimized PRP concentration and nanofiber degradation (and thus bioactive factor release) seems to be as a promising approach for appropriate composite systems for bone tissue regeneration.…”

Section: Discussionmentioning

confidence: 99%

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Collagen/hydroxyapatite scaffold enriched with polycaprolactone nanofibers, thrombocyte‐rich solution and mesenchymal stem cells promotes regeneration in large bone defect in vivo

Prosecká

Rampichová

Litvinec

et al. 2014

J Biomedical Materials Res

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A three-dimensional scaffold of type I collagen and hydroxyapatite enriched with polycaprolactone nanofibers (Coll/HA/PCL), autologous mesenchymal stem cells (MSCs) in osteogenic media, and thrombocyte-rich solution (TRS) was an optimal implant for bone regeneration in vivo in white rabbits. Nanofibers optimized the viscoelastic properties of the Coll/HA scaffold for bone regeneration. MSCs and TRS in the composite scaffold improved bone regeneration. Three types of Coll/HA/PCL scaffold were prepared: an MSC-enriched scaffold, a TRS-enriched scaffold, and a scaffold enriched with both MSCs and TRS. These scaffolds were implanted into femoral condyle defects 6 mm in diameter and 10-mm deep. Untreated defects were used as a control. Macroscopic and histological analyses of the regenerated tissue from all groups were performed 12 weeks after implantation. The highest volume and most uniform distribution of newly formed bone occurred in defects treated with scaffolds enriched with both MSCs and TRS compared with that in defects treated with scaffolds enriched by either component alone. The modulus of elasticity in compressive testing was significantly higher in the Coll/HA/PCL scaffold than those without nanofibers. The composite Coll scaffold functionalized with PCL nanofibers and enriched with MSCs and TRS appears to be a novel treatment for bone defects.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Collagen/hydroxyapatite scaffold enriched with polycaprolactone nanofibers, thrombocyte‐rich solution and mesenchymal stem cells promotes regeneration in large bone defect in vivo

Prosecká

Rampichová

Litvinec

et al. 2014

J Biomedical Materials Res

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“…In the presence of hydroxyapatite and silica coated hydroxyapatite scaffolds, platelet poor plasma was able to stimulate the osteogenic differentiation of goat MSCs greater than the platelet lysates. 198 …”

Section: Bone Regeneration Processmentioning

confidence: 99%

Application of platelet-rich plasma with stem cells in bone and periodontal tissue engineering

2016

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Presently, there is a high paucity of bone grafts in the United States and worldwide. Regenerating bone is of prime concern due to the current demand of bone grafts and the increasing number of diseases causing bone loss. Autogenous bone is the present gold standard of bone regeneration. However, disadvantages like donor site morbidity and its decreased availability limit its use. Even allografts and synthetic grafting materials have their own limitations. As certain specific stem cells can be directed to differentiate into an osteoblastic lineage in the presence of growth factors (GFs), it makes stem cells the ideal agents for bone regeneration. Furthermore, platelet-rich plasma (PRP), which can be easily isolated from whole blood, is often used for bone regeneration, wound healing and bone defect repair. When stem cells are combined with PRP in the presence of GFs, they are able to promote osteogenesis. This review provides in-depth knowledge regarding the use of stem cells and PRP in vitro, in vivo and their application in clinical studies in the future.

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“…However, these effects were dependent on the PRP composition [10]. Somehow data become controversial when in vivo settings are compared, since opposite outcomes are equally available in the scientific literature in favor [11][12][13][14][15][16][17][18][19][20][21] or against [19,[22][23][24][25][26][27] the addition of PRP to biologic and synthetic graft materials for bone regeneration purposes. Interestingly, the association of PRP with mesenchymal stem cells showed mostly satisfying results [28,29] in the field.…”

Section: Introductionmentioning

confidence: 99%

Cytokine, chemokine, and growth factor profile of platelet-rich plasma

et al. 2016

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During wound healing, biologically active molecules are released from platelets. The rationale of using platelet-rich plasma (PRP) relies on the concentration of bioactive molecules and subsequent delivery to healing sites. These bioactive molecules have been seldom simultaneously quantified within the same PRP preparation. In the present study, the flexible Bio-Plex system was employed to assess the concentration of a large range of cytokines, chemokines, and growth factors in 16 healthy volunteers so as to determine whether significant baseline differences may be found. Besides IL-1b, IL-1ra, IL-4, IL-6, IL-8, IL-12, IL-13, IL-17, INF-γ, TNF-α, MCP-1, MIP-1a, RANTES, bFGF, PDGF, and VEGF that were already quantified elsewhere, the authors reported also on the presence of IL-2, IL-5, IL-7, IL-9, IL-10, IL-15 G-CSF, GM-CSF, Eotaxin, CXCL10 chemokine (IP-10), and MIP 1b. Among the most interesting results, it is convenient to mention the high concentrations of the HIV-suppressive and inflammatory cytokine RANTES and a statistically significant difference between males and females in the content of PDGF-BB. These data are consistent with previous reports pointing out that gender, diet, and test system affect the results of platelet function in healthy subjects, but seem contradictory when compared to other quantification assays in serum and plasma. The inconsistencies affecting the experimental results found in literature, along with the variability found in the content of bioactive molecules, urge further research, hopefully in form of randomized controlled clinical trials, in order to find definitive evidence of the efficacy of PRP treatment in various pathologic and regenerative conditions.

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Calcium phosphate bone cement with 10wt% platelet-rich plasma in vitro and in vivo

Cited by 31 publications

References 35 publications

Collagen/hydroxyapatite scaffold enriched with polycaprolactone nanofibers, thrombocyte‐rich solution and mesenchymal stem cells promotes regeneration in large bone defect in vivo

Collagen/hydroxyapatite scaffold enriched with polycaprolactone nanofibers, thrombocyte‐rich solution and mesenchymal stem cells promotes regeneration in large bone defect in vivo

Application of platelet-rich plasma with stem cells in bone and periodontal tissue engineering

Cytokine, chemokine, and growth factor profile of platelet-rich plasma

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