Calcium carbonate hydrogel construct with cynnamaldehyde incorporated to control inflammation during surgical procedure

Abstract

The incorporation of CaCO hydrogel has been proven to enhance the bone biological activity of Plaster of Paris (POP) and to decrease its degradability. However, the installation of this bone substitute in a bone defect will still be associated with an inflammatory response. In this study, the influence of cinnamaldehyde as anti-inflammatory agent, was investigated. In addition, it is known that aldehyde chains of cinnamaldehyde may also act as crosslinking agent and function as a plastisizer to the CaCO hydrogel construct. Therefore, different concentrations of cinnamaldehyde were added to CaCO hydrogel and the effect on the diametral tensile strength, age swelling, gel fractination, cinnamaldehyde release, antimicrobial effect, and cell cytotoxycity were investigated. The incorporation of cinnamaldehyde was found to decrease the age swelling and degradation rate of CaCO hydrogel and to have no toxic effect to human gingival fibroblast cells. Moreover, the incorporation of cinnamaldehyde essential oil into the CaCO hydrogel was beneficial and acted as an antiinflammatory agent. Further research in vivo is warranted to determine the final favorable effect of cinnamaldehyde incorporated CaCO hydrogel in POP to provide a bone substitute. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 768-774, 2016.

Activity of Cinnamaldehyde on Quorum Sensing and Biofilm Susceptibility to Antibiotics in Pseudomonas aeruginosa

“…The cytotoxic effect of CAD must also be considered. Previous research demonstrated that 57 mM CAD did not show inhibitory effects on primary human T cells or macrophages [43] or that 4% (303 mM) CAD in calcium carbonate hydrogel had no cytotoxic effect on human gingival fibroblast cells [44]. We evaluated CAD concentrations substantially lower than these (e.g., around the MIC of 11.8 mM), so we predict that our tested CAD concentrations would not be cytotoxic.…”

Preparation of a calcium carbonate‐based bone substitute with cinnamaldehyde crosslinking agent with potential anti‐inflammatory properties

“…Despite these advantages, CS cements show a too fast resorption rate and are unable to provide a long‐term 3D framework during the osteogenesis process . To solve this problem, a biocompatible and osteoconductive hydrogel calcium carbonate has been incorporated into CS formulations …”

“…[4][5][6] To solve this problem, a biocompatible and osteoconductive hydrogel calcium carbonate has been incorporated into CS formulations. 7,8 A bone replacing material comes not only in contact with bone, but also with the surrounding soft tissues, such as periosteum, connective tissue, and muscles. 9 Soft tissues are generally considered to show a more severe inflammatory response than bone.…”

Effect of Dehydrothermal Treatment on the Mechanical Properties and Biocompatibility of Plaster of Paris–CaCO3 Hydrogel Loaded With Cinnamaldehyde for Biomedical Purposes

“…On the other hand, CA is also known to have antimicrobial properties. 17,44,45 The addition of CA at different concentrations (4%, 6%, 8%, and 10%) inside the composite resulted in a decrease in the swelling ratio and degradation rate. 45 Swelling behavior was influenced by crosslink density, while swelling capacity can be modified by chemical or physical crosslink methods.…”

“…17,44,45 The addition of CA at different concentrations (4%, 6%, 8%, and 10%) inside the composite resulted in a decrease in the swelling ratio and degradation rate. 45 Swelling behavior was influenced by crosslink density, while swelling capacity can be modified by chemical or physical crosslink methods. 46 The SEM results of hydrogel with CA showed a smaller pore size than the other ones without CA.…”