Calcitonin gene-related peptide- and adrenomedullin-induced facilitation of calcium current in submandibular ganglion

Abstract

Objective: The control of saliva secretion is mainly under parasympathetic control. The submandibular ganglion (SMG) is a parasympathetic ganglion which receives inputs from preganglionic cholinergic neurons, and innervates the submandibular salivary gland to control saliva secretion. The aim of this study was to investigate if adrenomedullin (ADM) and/or calcitonin gene-related peptide (CGRP) modulate voltage-dependent calcium channel (VDCCs) current (ICa) in SMG.Design: The profile of CGRP and ADM actions in SMG was studied using the whole-cell configuration of the patch-clamp technique.Results: Both ADM and CGRP facilitated ICa. These facilitations were attenuated by intracellular dialysis of the anti-G α s-protein and pretreatment of SQ22536 (an adenylate cyclase inhibitor).Conclusions: ADM and CGRP facilitates VDCCs mediated by G α s-protein and adenylate cyclase in SMG.3

Plasmid pLXSN-Mediated Adrenomedullin Gene Therapy for Cerebral Vasospasm Following Subarachnoid Hemorrhage in Rats

“…The second role is BBB regulation: research [15] found that astrocyte secretion factor can induce cerebral vascular endothelial cells to generate ADM, participate in the differentiation and proliferation of vascular endothelial cells, and regulate BBB phenotype. ADM has a cAMP-like effect, it can reduce the permeability and phagocytosis of cerebral vascular endothelial cells and increase P-protein levels, playing an important role in accurate regulation of BBB through autocrine or paracrine [16]. The third role is stabilization of homeostasis: in the central nervous system, AM13-52, the ADM active fragment, can act on the adenohypophysis cells, inhibiting the secretion and release of adrenocorticotropic hormone through the inhibition of basic and hormone-stimulating hormone in a dose-dependent manner [17].…”

Adrenomedullin Facilitates Calcium Channel Currents in Osteoblasts

“…L-type VDCCs possess several consensus protein kinase A (PKA) and protein kinase C (PKC) phosphorylation sites and physiological studies have demonstrated channel facilitation by these enzymes 14) . We previously demonstrated that ADM facilitates VDCCs mediated by PKA in submandibular ganglion 8) . We have also demonstrated that ADM facilitates VDCCs involving mitogenactivated protein kinase in nucleus tractus solitarius 11) .…”

“…Modulation of VDCCs by CGRP and ADM in various types of cells has been described previously 8,11,19) . However, the effect of ADM on VDCCs in osteoblasts remains to be clarified.…”

Aging-Related Metabolic Dysfunction in the Salivary Gland: A Review of the Literature

“…Both neuronal and endothelial nitric oxide synthase produce the free radical nitric oxide, which can influence saliva and protein secretion [ 49 ]. CGRP can modulate voltage-dependent calcium channels, one of the key regulators of calcium influx, which are involved in saliva secretion [ 50 ]. A summary of the role of several neurochemicals is presented in Table 2 .…”