Adrenomedullin Facilitates Calcium Channel Currents in Osteoblasts

Tazaki, Masakazu; Endoh, Takayuki; Kobayashi, Hiroshi; Nobushima, Hiromi; Shibukawa, Yoshiyuki; Tsumura, Maki; Sato, Masaki; Ubaidus, Sobhan; Sueishi, Katsuo

doi:10.2209/tdcpublication.53.203

Cited by 6 publications

(6 citation statements)

References 19 publications

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“…Adrenomedullin (ADM) is a peptide hormone that in humans is encoded by the ADM gene. ADM was reported to inhibit the osteoclastogenesis [ 47 ], while stimulate osteoblast growth and proliferation [ 48 ]. Up-regulation of the ADM system occurred early and took part in the adaptative changes occurring during simulated microgravity conditions [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

GeneChip Expression Profiling Reveals the Alterations of Energy Metabolism Related Genes in Osteocytes under Large Gradient High Magnetic Fields

et al. 2015

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The diamagnetic levitation as a novel ground-based model for simulating a reduced gravity environment has recently been applied in life science research. In this study a specially designed superconducting magnet with a large gradient high magnetic field (LG-HMF), which can provide three apparent gravity levels (μ-g, 1-g, and 2-g), was used to simulate a space-like gravity environment. Osteocyte, as the most important mechanosensor in bone, takes a pivotal position in mediating the mechano-induced bone remodeling. In this study, the effects of LG-HMF on gene expression profiling of osteocyte-like cell line MLO-Y4 were investigated by Affymetrix DNA microarray. LG-HMF affected osteocyte gene expression profiling. Differentially expressed genes (DEGs) and data mining were further analyzed by using bioinfomatic tools, such as DAVID, iReport. 12 energy metabolism related genes (PFKL, AK4, ALDOC, COX7A1, STC1, ADM, CA9, CA12, P4HA1, APLN, GPR35 and GPR84) were further confirmed by real-time PCR. An integrated gene interaction network of 12 DEGs was constructed. Bio-data mining showed that genes involved in glucose metabolic process and apoptosis changed notablly. Our results demostrated that LG-HMF affected the expression of energy metabolism related genes in osteocyte. The identification of sensitive genes to special environments may provide some potential targets for preventing and treating bone loss or osteoporosis.

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Section: Discussionmentioning

confidence: 99%

GeneChip Expression Profiling Reveals the Alterations of Energy Metabolism Related Genes in Osteocytes under Large Gradient High Magnetic Fields

et al. 2015

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“…Adrenomedullin is expressed mostly in osteoblasts and shows activity via a paracrine/autocrine mechanism [ 165 ]. Dissimilar to other peptides of calcitonin family, adrenomedullin shows uncertain impacts on osteoblast cAMP levels showing that adrenomedullin in these cells activates ERK1/2 and voltage-dependent calcium channels [ 166 , 167 ]. Additionally, adrenomedullin exhibited no direct binding to IGF-1R, and like amylin, its effect of proliferation in osteoblastic cells shows dependency on IGF-1R [ 156 ].…”

Section: In Vitro and In Vivo mentioning

confidence: 99%

Calcitonin and Bone Physiology: In Vitro, In Vivo, and Clinical Investigations

Xie

Guo

Kanwal

et al. 2020

International Journal of Endocrinology

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Calcitonin was discovered as a peptide hormone that was known to reduce the calcium levels in the systemic circulation. This hypocalcemic effect is produced due to multiple reasons such as inhibition of bone resorption or suppression of calcium release from the bone. Thus, calcitonin was said as a primary regulator of the bone resorption process. This is the reason why calcitonin has been used widely in clinics for the treatment of bone disorders such as osteoporosis, hypercalcemia, and Paget’s disease. However, presently calcitonin usage is declined due to the development of efficacious formulations of new drugs. Calcitonin gene-related peptides and several other peptides such as intermedin, amylin, and adrenomedullin (ADM) are categorized in calcitonin family. These peptides are known for the structural similarity with calcitonin. Aside from having a similar structure, these peptides have few overlapping biological activities and signal transduction action through related receptors. However, several other activities are also present that are peptide specific. In vitro and in vivo studies documented the posttreatment effects of calcitonin peptides, i.e., positive effect on bone osteoblasts and their formation and negative effect on osteoclasts and their resorption. The recent research studies carried out on genetically modified mice showed the inhibition of osteoclast activity by amylin, while astonishingly calcitonin plays its role by suppressing osteoblast and bone turnover. This article describes the review of the bone, the activity of the calcitonin family of peptides, and the link between them.

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“…Initially extracted from the pheochromocytoma tissue excised in a surgery (1), adrenomedullin (ADM) is a polypeptide sharing homology with calcitonin gene-related peptide and has functions of vascular dilation and diuretic activity (2). In a healthy individual, a considerable plasmatic concentration of ADM is involved in the regulation of plasma (3).…”

Section: Introductionmentioning

confidence: 99%

Expression of adrenomedullin in rats after spinal cord injury and intervention effect of recombinant human erythropoietin

Zhao

et al. 2016

Experimental and Therapeutic Medicine

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The expression of adrenomedullin (ADM) in injured tissue of rat spinal cord was observed and the effect of recombinant human erythropoietin was analyzed. A total of 45 Sprague-Dawley rats were selected and divided into 3 equal groups including, a sham-operation group in which rats received an excision of vertebral plate; a spinal cord injury model group and a recombinant human erythropoietin group in which rats with spinal cord injury received a caudal vein injection of 300 units recombinant human erythropoietin after injury. Hematoxylin and eosin staining was performed to observe the spinal cord injury conditions. Immunohistochemical staining was performed to observe the expression of ADM. Pathologic changes in the group of recombinant human erythropoietin at various times were significantly less severe than those in the group of spinal cord injury model. The expression of ADM was increased particularly in the group of recombinant human erythropoietin (P<0.01). The improved Tarlov scores of the group of spinal cord injury model and the group of recombinant human erythropoietin were lower than those of the sham-operation group at 3, 6 and 9 days (P<0.01). Thus, the recombinant human erythropoietin is capable of alleviating the secondary injury of spinal cord. One of the mechanisms may be achieved by promoting the increase of ADM expression.

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Adrenomedullin Facilitates Calcium Channel Currents in Osteoblasts

Cited by 6 publications

References 19 publications

GeneChip Expression Profiling Reveals the Alterations of Energy Metabolism Related Genes in Osteocytes under Large Gradient High Magnetic Fields

GeneChip Expression Profiling Reveals the Alterations of Energy Metabolism Related Genes in Osteocytes under Large Gradient High Magnetic Fields

Calcitonin and Bone Physiology: In Vitro, In Vivo, and Clinical Investigations

Expression of adrenomedullin in rats after spinal cord injury and intervention effect of recombinant human erythropoietin

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