Calbindin-D28K and the peptidergic neuroendocrine system in rat gut: an immunohistochemical study

Resibois, A

doi:10.1016/0248-4900(88)90109-8

Cited by 8 publications

(11 citation statements)

References 27 publications

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“…Intestines (duodenum, jejunum, and/or ileon and colon) were dissected, opened longitudinally and rinsed in phosphate buffer saline pH 7.4 before fixation. Intestine fragments were fixed either in Helly fixative [10] for 5 h or 2 h in 40/0 cold phosphate buffered formaldehyde freshly prepared from paraformaldehyde and postfLxed overnight in Bouin Hollande Sublim6 (PAF-BHS) [29]. After dehydration and paraffin embedding, 5 ~m-thick sections were prepared for histochemical study.…”

Section: Preparation Of Tissuementioning

confidence: 99%

“…In pig, calbindin D9k was observed in duodenal absorptive ceils [2] while in rat it was described in duodenum [20] but also in jejunum and caecum [24]. Studies on mammals do not indicate the presence of calbindin D28k in their absorptive cells although calbindin D28k was found in endocrine cells of several species [7,29]. Because of this discrepancy, we decided to investigate if indeed calbindin D28k could ever be encountered in mammalian absorptive cells using intestine from goat, rat, mouse, pig, which is thought to be fairly similar to human [4], and jerboa as an unsual rodent.…”

Section: Introductionmentioning

confidence: 99%

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Calbindin D28k in mammalian intestinal absorptive cells: immunohistochemical evidence

Pochet¹,

Blachier²,

Gangji³

et al. 1990

Biology of the Cell

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Calbindin D28k and D9k are two cytosolic calcium-binding proteins abundant in intestinal absorptive cells which appear to play a role in calcium translocation. Until today, calbindin D28k was found in avian and reptilian absorptive cells but not in mammalian ones. We have described the presence of calbindin D28k-immunoreactivity in intestinal absorptive cells of pig and jerboa (Jaculus jaculus). Pig calbindin D28k-immunoreactive absorptive cells were prominent in duodenum, they were scattered along the villi and nearly absent in the crypts. Jerboa labelled absorptive cells were located along the colonic mucosal surface. No calbindin D28k could be detected in mouse, rat and goat absorptive cells. Topography of calbindin D28k absorptive cells was compared with calbindin D9k distribution. Our results confirmed the data of the literature showing a gradient of labelling increasing from the crypt to the top of the villus and no positive endocrine cell. Young (48 h old) pigs did not expressed calbindin D28k in absorptive cells although calbindin D9k was detected. Calbindin D28K was also observed in endocrine cells which were numerous in pig and goat duodenum and very rare in mouse and jerboa. Western blot experiments confirmed the presence of calbindin D28k in the adult pig intestine, in the jerboa colon and the absence of cross-reactivity between calbindin D28k antibody and calbindin D9k.

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Section: Preparation Of Tissuementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Calbindin D28k in mammalian intestinal absorptive cells: immunohistochemical evidence

Pochet¹,

Blachier²,

Gangji³

et al. 1990

Biology of the Cell

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“…The neuropeptide calbindin (CB) was first identified in the intestines of the chicken (Wasserman et al 1966) and later studies have identified CB within intramural and extrinsic enteric neurons (Li and Masuko 1997, 2001; Resibois et al 1988; Wojtkiewicz et al 2011). However, the role of CB in the GI tract has not yet been fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

Proliferative Enteropathy (PE)—Induced Changes in the Calbindin-Immunoreactive (CB-IR) Neurons of Inferior Mesenteric Ganglion Supplying the Descending Colon in the Pig

et al. 2011

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A subpopulation of the pig inferior mesenteric ganglia (IMG) neurons projecting to the colon exhibit calbindin-like immunoreactivity. It is not known if there are any changes in the chemical coding patterns of these neurons during porcine proliferative enteropathy (PE). To answer this question, juvenile Large White Polish pigs with clinically diagnosed Lawsonia intracellularis infection (PE; n = 3) and a group of uninfected controls (C; n = 3) were compared. The retrograde tracer fast blue (FB) was injected into the descending colons of all animals and then tissue comprising IMGs from both groups was processed for double-labeling immunofluorescence with calbindin-D28k (CB) in combination with either tyrosine hydroxylase (TH), neuropeptide Y (NPY), somatostatin (SOM), vasoactive intestinal polypeptide (VIP), nitric oxide synthase, Leu-enkephalin, substance P, vesicular acetylcholine transporter, galanin, or pituitary adenylate cyclase-activating polypeptide. Immunohistochemistry revealed changes in the chemical coding pattern of calbindin-immunoreactive neurons in the inferior mesenteric ganglia of the pig. In control animals, FB/CB-positive neurons were immunoreactive to TH, NPY, SOM, and VIP. In the experimental group, TH-expressing neurons were unaffected, NPY-expressing neurons were increased, whereas the number of neurons immunoreactive to SOM or VIP was reduced. Changes in chemical coding of CB neurons during PE may play an important role in adaptation of these IMG cells under pathological conditions.

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“…In the guinea-pig GI tract, CB-positive neurons function as intrinsic primary afferent neurons or as interneurons. The somata of CB-containing primary afferent neurons in the intestine of several species are located within the myenteric plexus and project to the epithelium [4,9,14]. Based on these previous studies, CB-IR neurons in the myenteric neurons of goat stomach may also be sensory neurons that project to the epithelium.…”

Section: Discussionmentioning

confidence: 99%

“…Among them, calbindin D-28k (CB) and calretinin (CR) are observed in ENS neurons characterized by a distinct morphology and specific electrophysiological properties. Furthermore, CaBPs also modulate the release or action of SP and CGRP [4,5,14]. …”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical localization of calcium binding proteins and some neurotransmitters in myenteric plexus of goat stomach

Lee

Nam

2006

J Vet Sci

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To understand the neurochemical properties of the gastric myenteric plexus of ruminants, the expression patterns of calbindin D-28k (CB), calretinin (CR), substance P (SP) and calcitonin gene-related peptide (CGRP) were explored in the Korean native goat. In gastric myenteric plexus, CB and SP immunoreactivity were observed in round- or oval-shaped neurons. CR and CGRP immunoreactivity were detected only in the nerve fibers. This immunohistochemical localization of CB, CR, CGRP and SP in the myenteric plexus of the goat stomach exhibited species-specific patterns. These findings suggest that these substances may be directly or indirectly related to the gastric functions of the goat stomach.

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Calbindin-D28K and the peptidergic neuroendocrine system in rat gut: an immunohistochemical study

Cited by 8 publications

References 27 publications

Calbindin D28k in mammalian intestinal absorptive cells: immunohistochemical evidence

Calbindin D28k in mammalian intestinal absorptive cells: immunohistochemical evidence

Proliferative Enteropathy (PE)—Induced Changes in the Calbindin-Immunoreactive (CB-IR) Neurons of Inferior Mesenteric Ganglion Supplying the Descending Colon in the Pig

Immunohistochemical localization of calcium binding proteins and some neurotransmitters in myenteric plexus of goat stomach

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