Calbindin D28k in mammalian intestinal absorptive cells: immunohistochemical evidence

Abstract

Calbindin D28k and D9k are two cytosolic calcium-binding proteins abundant in intestinal absorptive cells which appear to play a role in calcium translocation. Until today, calbindin D28k was found in avian and reptilian absorptive cells but not in mammalian ones. We have described the presence of calbindin D28k-immunoreactivity in intestinal absorptive cells of pig and jerboa (Jaculus jaculus). Pig calbindin D28k-immunoreactive absorptive cells were prominent in duodenum, they were scattered along the villi and nearly absent in the crypts. Jerboa labelled absorptive cells were located along the colonic mucosal surface. No calbindin D28k could be detected in mouse, rat and goat absorptive cells. Topography of calbindin D28k absorptive cells was compared with calbindin D9k distribution. Our results confirmed the data of the literature showing a gradient of labelling increasing from the crypt to the top of the villus and no positive endocrine cell. Young (48 h old) pigs did not expressed calbindin D28k in absorptive cells although calbindin D9k was detected. Calbindin D28K was also observed in endocrine cells which were numerous in pig and goat duodenum and very rare in mouse and jerboa. Western blot experiments confirmed the presence of calbindin D28k in the adult pig intestine, in the jerboa colon and the absence of cross-reactivity between calbindin D28k antibody and calbindin D9k.

Localization of calbindin D28K-like immunoreactivity in fish gill: a light microscopic and immunoelectron histochemical study

“…Calbindin D28K distribution in the sill asrees with the immunohistochcmical findings reported by Parmentier [4] and Pochet et al [5] in the gut mucosa, i.e., immunolabelling was mainly restricted to the NE cells.…”

“…The antisera have been successfully used for immunohistochemistry and West ern blot experiments in epithelia of various vertebrate species [4,5].…”

“…It was first described by Wasserman and Taylor [1] in the chick intesti nal mucosa. Certain neurons of the central nervous system, and enterocytes of mam malian and non-mammalian vertebrate species contain high concentrations of this protein [2][3][4][5]. Calbindin D28K synthesis in intestine, kidney and pancreas is regulated by 1,25-dihydroxyvitamin D-3.…”

“…Recently, calbindin D28K was detected by immunohistochemistry in the absorptive cells and the neuroendocrine (NE) cells of gut epithelium of higher and lower verte brates [3][4][5]. The protein may play a key role in the calcium transport in these tissues.…”

Peptide binding proclivities of calcium loaded calbindin‐D28k

“…Calbindin‐D28k (261 residues, ∼30 kDa) is a member of the calmodulin superfamily of intracellular Ca 2+ ‐binding proteins [3]. It plays roles in protecting against excitotoxic cell death in hippocampal neurons, and in transcellular Ca 2+ movement in intestinal absorptive cells and kidney distal tubules [5–7]. Calbindin‐D28k also controls insulin release in pancreatic islet cells and co‐localizes and interacts with the plasma membrane Ca 2+ pump in the nervous system [6,8].…”