Caffeine prevents LPS-induced inflammatory responses in RAW264.7 cells and zebrafish

Hwang, Jang Yeon; Kim, Kui-Jin; Ryu, Su-Jung; Lee, Boo-Yong

doi:10.1016/j.cbi.2016.01.020

Cited by 103 publications

(65 citation statements)

References 30 publications

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“…When an in vitro cell viability evaluation was performed, PF was not found to exhibit an evident toxic effect on RAW 264.7 murine macrophages in the concentration range evaluated (Figure ). We established an inflammatory model of RAW 264.7 murine macrophages, which is often employed in anti‐inflammatory research (Gutierrez & Hoyo‐Vadillo, ; Hwang, Kim, Ryu, & Lee, ). Many studies have shown that the NF‐κB signaling pathway plays a vital role in inflammation (DiDonato, Mercurio, & Karin, ; Hoesel & Schmid, ).…”

Section: Discussionmentioning

confidence: 99%

Paeoniflorin alleviates lipopolysaccharide‐induced disseminated intravascular coagulation by inhibiting inflammation and coagulation activation

Fang

et al. 2020

Drug Development Research

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Lipopolysaccharide (LPS) is a toxic component of the outer membrane of gram-negative bacteria that can activate the blood coagulation system, leading to disseminated intravascular coagulation (DIC). DIC is a syndrome characterized by thromboembolism and multiple organ failure. Herein, the beneficial effect of paeoniflorin (PF) on the alleviation of LPS-induced DIC was investigated with an experimental DIC mouse model. Briefly, mice were randomly divided into the following six groups: (1) control; (2) LPS; (3) heparin;(4) low-PF treatment; (5) medium-PF treatment; and (6) high-PF treatment. The histological morphology of the liver and kidney was observed, and the coagulation indicators (such as prothrombin time), function indicators (such as alanine transferase), and inflammatory factors (such as TNF-α) were detected. Additionally, an in vitro cell inflammation model using RAW 264.7 murine macrophages was established. Activation of the nuclear factor kappa B (NF-κB) signaling pathway and tumor necrosis factor-α (TNF-α) were determined by western blotting. Based on our findings, PF could significantly improve the histological morphology of the liver and kidney, indicating that PF protects the liver and kidney against damage induced by LPS. Additionally, PF improved the function and coagulation indicators and reduced the production of inflammatory factors. In vitro, PF inhibited the expression of TNF-α by suppressing NF-κB signaling pathway activation.Collectively, our findings support the hypothesis that PF has anti-inflammatory and anticoagulation effects for the alleviation of LPS-induced DIC. PF is thus a potential cotreatment option for DIC. K E Y W O R D S disseminated intravascular coagulation (DIC), heparin, lipopolysaccharide (LPS), paeoniflorin (PF), tumor necrosis factor-α (TNF-α)

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Section: Discussionmentioning

confidence: 99%

Paeoniflorin alleviates lipopolysaccharide‐induced disseminated intravascular coagulation by inhibiting inflammation and coagulation activation

Fang

et al. 2020

Drug Development Research

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“…Zebrafish (Danio rerio) has been increasingly used as an in vivo animal model for assessment of drug efficacy, toxicity and safety (Hwang et al 2016). This is due to its http://e-algae.org…”

Section: Introductionmentioning

confidence: 99%

Protective effect of gallic acid derivatives from the freshwater green alga Spirogyra sp. against ultraviolet B-induced apoptosis through reactive oxygen species clearance in human keratinocytes and zebrafish

Wang¹,

Ryu²,

Kim³

et al. 2017

ALGAE

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“…Tea catechins, especially EGCG, have been shown to suppress the levels of pro‐inflammatory cytokines through down‐regulating nuclear factor‐κB (NF‐κB) pathway (Ravindranath and Ravindranath ). Furthermore, caffeine alleviated LPS‐induced inflammatory responses through inhibiting NF‐κB activation and p38 mitogen‐activated protein kinase (MAPK) phosphorylation (Hwang and others ). In addition to catechins and caffeine, anthocyanins have been reported to possess anti‐inflammatory activity (Vendrame and Klimis‐Zacas ).…”

Section: Resultsmentioning

confidence: 99%

Phytochemicals, Anti‐Inflammatory, Antiproliferative, and Methylglyoxal Trapping Properties of Zijuan Tea

Guan

et al. 2018

Journal of Food Science

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Caffeine prevents LPS-induced inflammatory responses in RAW264.7 cells and zebrafish

Cited by 103 publications

References 30 publications

Paeoniflorin alleviates lipopolysaccharide‐induced disseminated intravascular coagulation by inhibiting inflammation and coagulation activation

Paeoniflorin alleviates lipopolysaccharide‐induced disseminated intravascular coagulation by inhibiting inflammation and coagulation activation

Protective effect of gallic acid derivatives from the freshwater green alga Spirogyra sp. against ultraviolet B-induced apoptosis through reactive oxygen species clearance in human keratinocytes and zebrafish

Phytochemicals, Anti‐Inflammatory, Antiproliferative, and Methylglyoxal Trapping Properties of Zijuan Tea

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