Protective effect of gallic acid derivatives from the freshwater green alga Spirogyra sp. against ultraviolet B-induced apoptosis through reactive oxygen species clearance in human keratinocytes and zebrafish

Wang, Lei; Ryu, BoMi; Kim, Won‐Suk; Kim, Gwang Hoon; Jeon, You‐Jin

doi:10.4490/algae.2017.32.11.29

Cited by 37 publications

(32 citation statements)

References 35 publications

(32 reference statements)

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“…The FD-containing media were carefully removed and washed three times, followed by adding new media. MTT and DCFH-DA assays were conducted to measure the cell viability and intracellular ROS generation, respectively [16].…”

Section: Cell Viability and Ros Productionmentioning

confidence: 99%

(−)-Loliolide Isolated from Sargassum horneri Protects against Fine Dust-Induced Oxidative Stress in Human Keratinocytes

et al. 2020

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The emergence of fine dust (FD) among air pollutants has taken a toll during the past few decades, and it has provided both controversy and a platform for open conversation amongst world powers for finding sustainable solutions and effective treatments for health issues. The present study emphasizes the protective effects of (–)-loliolide (HTT) isolated from Sargassum horneri against FD-induced oxidative stress in human HaCaT keratinocytes. The purification of (–)-loliolide was carried out by centrifugal partition chromatography. HTT did not show any cytotoxicity, and it further illustrated the potential to increase cell viability by reducing the reactive oxygen species (ROS) production in FD-stimulated keratinocytes. Furthermore, HTT suppressed FD-stimulated DNA damage and the formation of apoptotic bodies, and it reduced the population of cells in the sub-G1 apoptosis phase. FD-induced apoptosis was advancing through the mitochondria-mediated apoptosis pathway. The cytoprotective effects of the HTT against FD-stimulated oxidative damage is mediated through squaring the nuclear factor E2-related factor 2 (Nrf2)-mediated heme oxygenase-1 (HO-1) pathway, dose-dependently increasing HO-1 and NAD(P)H dehydrogenase (quinone) 1 (NQO1) levels in the cytosol while concomitantly improving the nuclear translocation of Nrf2. Future studies could implement the protective functionality of HTT in producing pharmaceuticals that utilize natural products and benefit the diseased.

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Section: Cell Viability and Ros Productionmentioning

confidence: 99%

(−)-Loliolide Isolated from Sargassum horneri Protects against Fine Dust-Induced Oxidative Stress in Human Keratinocytes

et al. 2020

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“…UVB irradiation was imposed using a UVB meter (UV Lamp, VL-6LM, Vilber Lourmat, France) with a fluorescent bulb emitting at 280-320 nm with a peak at 313 nm. HaCaT cells were irradiated at a dose of 30 mJ/cm 2 of UVB in PBS (Wang et al 2017). After UVB irradiation, cells were incubated with serum-free DMEM media until analysis.…”

Section: Cell Culture and Uvb Irradiationmentioning

confidence: 99%

“…To determine the effect of HFPS on UVB-irradiated HaCaT cells, intracellular ROS levels, cell viability, and apoptosis body formation were measured. The intracellular ROS levels of UVB-irradiated HaCaT cells were determined using a DCF-DA assay and cell viability was measured by an MTT assay following the protocols described in a previous study (Wang et al 2017). Apoptosis body formation in UVBirradiated HaCaT cells was determined by Hoechst 33342 nuclear staining according to the method described by Wijesinghe et al (Wijesinghe et al 2013).…”

Section: Measurement Of the Effect Of Hfps On Uvb-irradiated Hacat Cellsmentioning

confidence: 99%

Protective effect of sulfated polysaccharides from a Celluclast-assisted extract of Hizikia fusiforme against ultraviolet B-induced photoaging in vitro in human keratinocytes and in vivo in zebrafish

Wang

Yang

et al. 2019

Mar Life Sci Technol

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Our previous study evaluated the in vitro and in vivo antioxidant activities of sulfated polysaccharides from a Celluclastassisted extract of Hizikia fusiforme (HFPS). The results indicate that HFPS possesses potent antioxidant activity and suggest the potential use of HFPS to combat photoaging. In this study, we investigated the ultraviolet (UV) protective effect of HFPS in vitro in keratinocytes (HaCaT cells) and in vivo in zebrafish. The results indicate that HFPS significantly reduced the level of intracellular reactive oxygen species (ROS) and improved the viability of UVB-irradiated HaCaT cells. In addition, HFPS remarkably decreased apoptosis formation in UVB-irradiated HaCaT cells in a dose-dependent manner. The in vivo test results also demonstrate that HFPS significantly reduced intracellular ROS levels, cell death, NO production, and lipid peroxidation levels in UVB-irradiated zebrafish in a dose-dependent manner. These results suggest that HFPS possesses strong in vitro and in vivo UV-protective effects, making it a potential ingredient in the cosmeceutical industry.

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“…Cell viability was quantified by a colorimetric MTT assay (Wang et al 2017;Wang et al 2018). Briefly, B16F10 cells were seeded in a 96-well plate and incubated for 24 h. The cells were treated with different concentrations of test samples and incubated for 72 h. MTT solution (50 μL, 2 mg/mL) was added to each well and incubated for 3 h. The supernatant was aspirated, 150 μL of DMSO was added to each well, and the absorbance was measured at 540 nm using a microplate reader.…”

Section: Cell Viability Assaymentioning

confidence: 99%

A mixture of seaweed extracts and glycosaminoglycans from sea squirts inhibits α-MSH-induced melanogenesis in B16F10 melanoma cells

et al. 2019

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Background: In the present study, the skin-whitening effects of a marine-sourced mixture that includes a fucoidanrich extract of Undaria pinnatifida (UPEF), a phlorotannin-rich extract of Ecklonia cava (ECE), and glycosaminoglycans (GAGs) from sea squirt skin were investigated. Methods: The whitening effects of the mixture and its components were evaluated by measuring the inhibition of mushroom tyrosinase and melanin synthesis in alpha-melanocyte-stimulating hormone (α-MSH)-stimulated B16F10 melanoma cells. Results: Each component alone markedly inhibited mushroom tyrosinase in a dose-dependent manner, and in α-MSH-stimulated B16F10 cells, they inhibited melanin synthesis and were cytotoxic. However, the whitening effects of UPEF, ECE, and GAGs in combination were greater than those of each component alone. A mixture in the ratio of 4:5:1 (UEG-451) showed the strongest activity without cytotoxicity. Further study suggested that UEG-451 inhibits α-MSH-stimulated melanogenesis in B16F10 cells by downregulating tyrosinase and tyrosinase-related proteins, such as TRP-1 and TRP-2, via the inhibition of MITF expression. Conclusions: These results suggest that mixing the different components at optimum ratios might be an effective way to improve their bioactivities and reduce toxicity and that UEG-451 possesses strong whitening effects that could be used in the cosmetic industry.

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Protective effect of gallic acid derivatives from the freshwater green alga Spirogyra sp. against ultraviolet B-induced apoptosis through reactive oxygen species clearance in human keratinocytes and zebrafish

Cited by 37 publications

References 35 publications

(−)-Loliolide Isolated from Sargassum horneri Protects against Fine Dust-Induced Oxidative Stress in Human Keratinocytes

(−)-Loliolide Isolated from Sargassum horneri Protects against Fine Dust-Induced Oxidative Stress in Human Keratinocytes

Protective effect of sulfated polysaccharides from a Celluclast-assisted extract of Hizikia fusiforme against ultraviolet B-induced photoaging in vitro in human keratinocytes and in vivo in zebrafish

A mixture of seaweed extracts and glycosaminoglycans from sea squirts inhibits α-MSH-induced melanogenesis in B16F10 melanoma cells

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