C2 feedstock-based biomanufacturing of value-added chemicals

Ma, Xiaoqiang; Hong, Liang; Panda, Smaranika; Fung, Vincent Kin Yuen; Zhou, Jie; Zhou, Kang

doi:10.1016/j.copbio.2021.08.017

Cited by 20 publications

(15 citation statements)

References 34 publications

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“…Furthermore, methanol and ethanol are considered some of the most useful substrates for obtaining complex chemical compounds. Therefore, these C1 or C2 chemicals have been used to produce valuable products such as polyhydroxyalkanoates, amino acids, organic acids, and other chemical compounds using diverse biocatalysts [ 1 , 2 ]. Thus, processes for the utilization of C1 or C2 compounds as substrates for value-added product manufacturing should be developed in further studies.…”

Section: Resultsmentioning

confidence: 99%

“…One- or two-carbon (C1 or C2) compounds have been utilized as attractive substrates for biotechnological production of valuable compounds because they are inexpensive and can be used as bio-renewable platform feedstock [ 1 , 2 ]. The production of value-added products from cheap C1 or C2 compounds via chemical processes has been extensively studied [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro One-Pot 3-Hydroxypropanal Production from Cheap C1 and C2 Compounds

Seo

Yeom

2022

IJMS

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One- or two-carbon (C1 or C2) compounds have been considered attractive substrates because they are inexpensive and abundant. Methanol and ethanol are representative C1 and C2 compounds, which can be used as bio-renewable platform feedstocks for the biotechnological production of value-added natural chemicals. Methanol-derived formaldehyde and ethanol-derived acetaldehyde can be converted to 3-hydroxypropanal (3-HPA) via aldol condensation. 3-HPA is used in food preservation and as a precursor for 3-hydroxypropionic acid and 1,3-propanediol that are starting materials for manufacturing biocompatible plastic and polytrimethylene terephthalate. In this study, 3-HPA was biosynthesized from formaldehyde and acetaldehyde using deoxyribose-5-phosphate aldolase from Thermotoga maritima (DERATma) and cloned and expressed in Escherichia coli for 3-HPA production. Under optimum conditions, DERATma produced 7 mM 3-HPA from 25 mM substrate (formaldehyde and acetaldehyde) for 60 min with 520 mg/L/h productivity. To demonstrate the one-pot 3-HPA production from methanol and ethanol, we used methanol dehydrogenase from Lysinibacillus xylanilyticus (MDHLx) and DERATma. One-pot 3-HPA production via aldol condensation of formaldehyde and acetaldehyde from methanol and ethanol, respectively, was investigated under optimized reaction conditions. This is the first report on 3-HPA production from inexpensive alcohol substrates (methanol and ethanol) by cascade reaction using DERATma and MDHLx.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In Vitro One-Pot 3-Hydroxypropanal Production from Cheap C1 and C2 Compounds

Seo

Yeom

2022

IJMS

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“…Here, we aim to present a brief summary on the very recent achievements focusing on the past 2–3 years in metabolic engineering and synthetic biology that use various NGFs for the production of biomass and/or value-added products. Unlike existing reviews focusing on either C1 ( Cotton et al, 2020 ; Jiang et al, 2021 ) or C2 ( Ma et al, 2022 ) feedstocks, we aim to compare the advantages and disadvantages of different C1 and C2 feedstocks and discuss their biotechnological potentials. We separately discuss natural metabolic pathways and synthetic routes of NGF assimilation using metabolic engineering and ALE strategies.…”

Section: Introductionmentioning

confidence: 99%

Microbial Utilization of Next-Generation Feedstocks for the Biomanufacturing of Value-Added Chemicals and Food Ingredients

Zhang

Ottenheim

Weingarten

et al. 2022

Front. Bioeng. Biotechnol.

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Global shift to sustainability has driven the exploration of alternative feedstocks beyond sugars for biomanufacturing. Recently, C1 (CO2, CO, methane, formate and methanol) and C2 (acetate and ethanol) substrates are drawing great attention due to their natural abundance and low production cost. The advances in metabolic engineering, synthetic biology and industrial process design have greatly enhanced the efficiency that microbes use these next-generation feedstocks. The metabolic pathways to use C1 and C2 feedstocks have been introduced or enhanced into industrial workhorses, such as Escherichia coli and yeasts, by genetic rewiring and laboratory evolution strategies. Furthermore, microbes are engineered to convert these low-cost feedstocks to various high-value products, ranging from food ingredients to chemicals. This review highlights the recent development in metabolic engineering, the challenges in strain engineering and bioprocess design, and the perspectives of microbial utilization of C1 and C2 feedstocks for the biomanufacturing of value-added products.

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“…Microbial fermentation uses biomass as a carbon-neutral resource instead of fossil resources or organic waste, thus providing a promising solution for reducing global carbon emission. Furthermore, some microorganisms can utilize carbon dioxide (CO 2 ) and convert it into valuable chemicals [ 1 , 2 , 3 , 4 , 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…Short-chain volatile fatty acids, especially acetic acid, are common microbial metabolites. They are useful as bulk chemicals in industries and have broad applications in food and pharmaceutical industries [ 1 , 2 , 5 , 6 , 8 ]. Acetic acid has an annual global demand of 10 million tons.…”

Section: Introductionmentioning

confidence: 99%

Removal of Acetic Acid from Bacterial Culture Media by Adsorption onto a Two-Component Composite Polymer Gel

Kato

Gotoh

Nakashimada

2022

Gels

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Organic acids, including acetic acid, are the metabolic products of many microorganisms. Acetic acid is a target product useful in the fermentation process. However, acetic acid has an inhibitory effect on microorganisms and limits fermentation. Thus, it would be beneficial to recover the acid from the culture medium. However, conventional recovery processes are expensive and environmentally unfriendly. Here, we report the use of a two-component hydrogel to adsorb dissociated and undissociated acetic acid from the culture medium. The Langmuir model revealed the maximum adsorption amount to be 44.8 mg acetic acid/g of dry gel at neutral pH value. The adsorption capacity was similar to that of an ion-exchange resin. In addition, the hydrogel maintained its adsorption capability in a culture medium comprising complex components, whereas the ion-exchange did not adsorb in this medium. The adsorbed acetic acid was readily desorbed using a solution containing a high salt concentration. Thus, the recovered acetic acid can be utilized for subsequent processes, and the gel-treated fermentation broth can be reused for the next round of fermentation. Use of this hydrogel may prove to be a more sustainable downstream process to recover biosynthesized acetic acid.

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C2 feedstock-based biomanufacturing of value-added chemicals

Cited by 20 publications

References 34 publications

In Vitro One-Pot 3-Hydroxypropanal Production from Cheap C1 and C2 Compounds

In Vitro One-Pot 3-Hydroxypropanal Production from Cheap C1 and C2 Compounds

Microbial Utilization of Next-Generation Feedstocks for the Biomanufacturing of Value-Added Chemicals and Food Ingredients

Removal of Acetic Acid from Bacterial Culture Media by Adsorption onto a Two-Component Composite Polymer Gel

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