Microbial Utilization of Next-Generation Feedstocks for the Biomanufacturing of Value-Added Chemicals and Food Ingredients

Zhang, Congqiang; Ottenheim, Christoph; Weingarten, M. David; Ji, LiangHui

doi:10.3389/fbioe.2022.874612

Cited by 26 publications

(39 citation statements)

References 130 publications

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“…When grown on CO 2 and H 2 only, acetogenic cultures also produce ethanol in substantial amount. Ethanol itself can be microbially upgraded (Zhang et al, 2022 ). As ethanol can be directly converted into acetyl‐CoA, it is suitable to produce acetyl‐CoA derivatives (Ma et al, 2022 ).…”

Section: Biological Two‐stage Processes For Products' Synthesis From ...mentioning

confidence: 99%

“…Up to date two options have emerged for developing the biotechnological valorization of methanol and formate: (i) engineering native methylotrophs or formatotrophs to improve their capacity for bioproduction, (ii) engineering synthetic methylotrophy or formatotrophy in established model species. Methylotrophic microorganisms are found in both prokaryotes and eukaryotes (Chen & Lan, 2020 ; Zhang et al, 2022 ). The eukaryotic methyltrophic yeast Pichia pastoris (reclassified as Komagataella phaffii ) can metabolize methanol as its sole carbon and energy source and is one of the most widely used host for the production of recombinant protein production (Ergün et al, 2021 ), SCP (Shay & Wegner, 1981 ), and several valuable compounds such as TCA cycle intermediates (Guo et al, 2021 ), terpenoids, polyketides, lovastatin – an antihypertensive compound – and its precursor monacolin (Liu et al, 2018 ).The most well studied methylotrophs include both Gram positive (e.g.…”

Section: Two‐stage Processes Using Products Of Abiotic Carbon Dioxide...mentioning

confidence: 99%

“…Industrial‐scale processes using methylotrophic bacteria have proven successful for providing large SCP amounts for human and animal feed. The examples from ICI (Windass et al, 1980 ) using Methylophilus methylotrophus , Hoechst/Uhde ( https://doi.org/10.1021/cen‐v056n020.p020 ) using Methylomonas clara and NorskHydro (Zhang et al, 2022 ) using Methylomonas methanolica illustrate that large‐scale methanol‐based processes are possible from the engineering point of view. Recent years have witnessed considerable progress in engineering synthetic methylotrophy in model bacteria such as in E. coli and S. cerevisiae (Keller et al, 2022 ; Kelso et al, 2022 ).…”

Section: Two‐stage Processes Using Products Of Abiotic Carbon Dioxide...mentioning

confidence: 99%

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Leveraging substrate flexibility and product selectivity of acetogens in two‐stage systems for chemical production

Ricci

Seifert²,

Bernacchi³

et al. 2022

Microbial Biotechnology

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Carbon dioxide (CO 2 ) stands out as sustainable feedstock for developing a circular carbon economy whose energy supply could be obtained by boosting the production of clean hydrogen from renewable electricity. H 2 ‐dependent CO 2 gas fermentation using acetogenic microorganisms offers a viable solution of increasingly demonstrated value. While gas fermentation advances to achieve commercial process scalability, which is currently limited to a few products such as acetate and ethanol, it is worth taking the best of the current state‐of‐the‐art technology by its integration within innovative bioconversion schemes. This review presents multiple scenarios where gas fermentation by acetogens integrate into double‐stage biotechnological production processes that use CO 2 as sole carbon feedstock and H 2 as energy carrier for products' synthesis. In the integration schemes here reviewed, the first stage can be biotic or abiotic while the second stage is biotic. When the first stage is biotic, acetogens act as a biological platform to generate chemical intermediates such as acetate, formate and ethanol that become substrates for a second fermentation stage. This approach holds the potential to enhance process titre/rate/yield metrics and products' spectrum. Alternatively, when the first stage is abiotic, the integrated two‐stage scheme foresees, in the first stage, the catalytic transformation of CO 2 into C 1 products that, in the second stage, can be metabolized by acetogens. This latter scheme leverages the metabolic flexibility of acetogens in efficient utilization of the products of CO 2 abiotic hydrogenation, namely formate and methanol, to synthesize multicarbon compounds but also to act as flexible catalysts for hydrogen storage or production.

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Section: Biological Two‐stage Processes For Products' Synthesis From ...mentioning

confidence: 99%

Section: Two‐stage Processes Using Products Of Abiotic Carbon Dioxide...mentioning

confidence: 99%

Section: Two‐stage Processes Using Products Of Abiotic Carbon Dioxide...mentioning

confidence: 99%

See 1 more Smart Citation

Leveraging substrate flexibility and product selectivity of acetogens in two‐stage systems for chemical production

Ricci

Seifert²,

Bernacchi³

et al. 2022

Microbial Biotechnology

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show abstract

“…One-carbon compounds are promising feedstocks because of its abundance in nature, non-food resources and potential for sustainability 1 . Enzymatic valorization of formaldehyde with high reactivity produces C2 alcohol and acid 2 , and multi-carbon compounds such dihydroxyacetone (C3) 3 and erythrulose(C4), 4 in line with the global trend towards more sustainable and eco-friendly resources (Scheme.…”

Section: Introductionmentioning

confidence: 99%

Biotransformation pathway and side reaction engineering for the whole-cell production of glycolic acid from formaldehyde

Kim

et al. 2022

Preprint

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A very simple biocatalytic system for the synthesis of the industrially relevant C2 chemicals (e.g., glycolic acid (3)) from formaldehyde (1) was established by whole-cell biocatalyst. The biocatalytic system consisted of an engineered glyoxylate carboligase from Escherichia coli K-12 (EcGCL) and an aldehyde dehydrogenase (e.g., AldA from E. coli K-12). To improve glycolic acid production, we have investigated metabolic engineering to regulate substrate consumption and product bypass and E. coli-based strain optimization. The target compound was produced at rates of up to 20.3 U/g dry cells with conversions up to 92%. This study will ensure the sustainability of the green energy industry by synthesizing valuable C2 chemical from a single carbon compound, formaldehyde.

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“…Microbes can be engineered to produce a variety of commodity chemicals and biofuels with high yields to maximize economic returns. Microbial design strategies have considered how cells allocate resources so as to redirect cellular energy toward making compounds of interest (9)(10)(11)(12)(13)(14)(15). Redirecting resources away from other processes can improve cellular product yields and thus decrease costs (16,17).…”

Section: Introductionmentioning

confidence: 99%

PKA regulatory subunit Bcy1 couples growth, lipid metabolism, and fermentation during anaerobic xylose growth inSaccharomyces cerevisiae

Wagner

Nightingale

Jen

et al. 2022

Preprint

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Organisms have evolved elaborate physiological pathways that regulate growth, proliferation, metabolism, and stress response. These pathways must be properly coordinated to elicit the appropriate response to an ever-changing environment. While individual pathways have been well studied in a variety of model systems, there remains much to uncover about how pathways are integrated to produce systemic changes in a cell, especially in dynamic conditions. We previously showed that deletion of Protein Kinase A (PKA) regulatory subunit BCY1 can decouple growth and metabolism in Saccharomyces cerevisiae engineered for anaerobic xylose fermentation, allowing for robust fermentation in the absence of division. This provides an opportunity to understand how PKA signaling normally coordinates these processes. Here, we integrated transcriptomic, lipidomic, and phosphor-proteomic responses upon a glucose to xylose shift across a series of strains with different genetic mutations promoting either coupled or decoupled xylose-dependent growth and metabolism. Together, results suggested that defects in lipid homeostasis limit growth in the bcy1Δ strain despite robust metabolism. To further understand this mechanism, we performed adaptive laboratory evolutions to re-evolve coupled growth and metabolism in the bcy1Δ parental strain. Genetic mutations in PKA subunit TPK1 and lipid regulator OPI1, among other genes underscored a role for lipid homeostasis, which was further supported by evolved changes in lipid profiles and gene expression. We suggest several models for how cells coordinate growth, metabolism, and other responses in budding yeast and how restructuring these processes enables anaerobic xylose utilization.

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Microbial Utilization of Next-Generation Feedstocks for the Biomanufacturing of Value-Added Chemicals and Food Ingredients

Cited by 26 publications

References 130 publications

Leveraging substrate flexibility and product selectivity of acetogens in two‐stage systems for chemical production

Leveraging substrate flexibility and product selectivity of acetogens in two‐stage systems for chemical production

Biotransformation pathway and side reaction engineering for the whole-cell production of glycolic acid from formaldehyde

PKA regulatory subunit Bcy1 couples growth, lipid metabolism, and fermentation during anaerobic xylose growth inSaccharomyces cerevisiae

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