C-terminal hydrophobic region leads PRSV P3 protein to endoplasmic reticulum

Eiamtanasate, Sarasate; Juříček, Miloslav; Yap, Yun‐Kiam

doi:10.1007/s11262-007-0114-z

Cited by 21 publications

(16 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These structures originated directly from the endoplasmic reticulum exit side. Localization of P3 (and, in addition P3-6K intermediate) in the endoplasmic reticulum was previously reported by Eiamtanasate et al [93]. Finally, the P3 punctate structure was found to traffic along the actin filaments and colocalize with the W-containing replication vesicles.…”

Section: Introductionsupporting

confidence: 70%

Sharka: The Past, The Present and The Future

Sochor

Babula

Adam

et al. 2012

Viruses

View full text Add to dashboard Cite

Members the Potyviridae family belong to a group of plant viruses that are causing devastating plant diseases with a significant impact on agronomy and economics. Plum pox virus (PPV), as a causative agent of sharka disease, is widely discussed. The understanding of the molecular biology of potyviruses including PPV and the function of individual proteins as products of genome expression are quite necessary for the proposal the new antiviral strategies. This review brings to view the members of Potyviridae family with respect to plum pox virus. The genome of potyviruses is discussed with respect to protein products of its expression and their function. Plum pox virus distribution, genome organization, transmission and biochemical changes in infected plants are introduced. In addition, techniques used in PPV detection are accentuated and discussed, especially with respect to new modern techniques of nucleic acids isolation, based on the nanotechnological approach. Finally, perspectives on the future of possibilities for nanotechnology application in PPV determination/identification are outlined.

show abstract

Section: Introductionsupporting

confidence: 70%

Sharka: The Past, The Present and The Future

Sochor

Babula

Adam

et al. 2012

Viruses

View full text Add to dashboard Cite

show abstract

“…The toxicity for bacteria in prokaryotic expression experiments and presence of one or two hydrophobic domains indicated the P3 affinity to cellular membranes (Rodríguez-Cerezo and Shaw, 1991;Šubr et al, 2000). Cytological localization of P3 in endoplasmatic reticulum confirmed this hypothesis (Eiamtanasate et al, 2007). Chung et al (2008) discovered an alternative shorter P3 variant in vivo, resulting from a +2 translation frameshift to so-called PIPO (pretty interesting potyviral ORF).…”

Section: Expression Strategy and Non-structural Proteinsmentioning

confidence: 81%

Unfolding the secrets of plum pox virus: from epidemiology to genomics

Šubr¹,

Glasa²

2013

View full text Add to dashboard Cite

Summary. -Over an approximately 80-year period since the description of the Sharka disease, the plum pox virus (PPV) has been thoroughly studied on various levels of the infection cycle. World-wide distribution of the virus, severity of the disease for the fruit industry with a potential to be further increased and discovery/ emergence of new strains make PPV the most epidemiologically important viral pathogen of stone fruit trees. The history of PPV research reflects the development of analytical methods applicable in plant virology. In particular the establishment of molecular biology with reverse genetics and improvement of DNA sequencing technology have further contributed to the increase in knowledge on PPV variability, evolution, replication and interaction with host plants. This review gives a comprehensive summary of PPV data accumulated progressively, from the biological characterization of the disease to recent attempts aimed at using the PPV-based vectors for expression of exogenous proteins in plants.

show abstract

“…TuMV is a (+) RNA virus in the family of Potyviridae (Mayo, 1995). Its 9.8-kb genome encodes at least 11 viral proteins, and among them are the membrane-associated P3 and 6K 2 proteins (Restrepo-Hartwig and Carrington, 1994;Eiamtanasate et al, 2007;Jiang et al, 2015). 6K 2 is a 6-kDa protein and, according to secondary structure predictions, is characterized by the presence of a 23-amino acid α-helix TMD, as well as a 19-and an 11-amino acid N-and C-terminal tail, respectively (Jiang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Turnip Mosaic Virus Uses the SNARE Protein VTI11 in an Unconventional Route for Replication Vesicle Trafficking

et al. 2018

View full text Add to dashboard Cite

Infection of plant cells by RNA viruses leads to the generation of organelle-like subcellular structures that contain the viral replication complex. During Turnip mosaic virus (TuMV) infection of Nicotiana benthamiana, the viral membrane protein 6K 2 plays a key role in the release of motile replication vesicles from the host endoplasmic reticulum (ER). Here, we demonstrate that 6K 2 contains a GxxxG motif within its predicted transmembrane domain that is vital for TuMV infection. Replacement of the Gly with Val within this motif inhibited virus production, and this was due to a relocation of the viral protein to the Golgi apparatus and the plasma membrane. This indicated that passage of 6K 2 through the Golgi apparatus is a dead-end avenue for virus infection. Impairing the fusion of transport vesicles between the ER and the Golgi apparatus by overexpression of the SNARE Sec22 protein resulted in enhanced intercellular virus movement. Likewise, expression of nonfunctional, Golgi-located synaptotagmin during infection enhanced TuMV intercellular movement. 6K 2 copurified with VTI11, a prevacuolar compartment SNARE protein. An Arabidopsis thaliana vti11 mutant was completely resistant to TuMV infection. We conclude that TuMV replication vesicles bypass the Golgi apparatus and take an unconventional pathway that may involve prevacuolar compartments/multivesicular bodies for virus infection.

show abstract

C-terminal hydrophobic region leads PRSV P3 protein to endoplasmic reticulum

Cited by 21 publications

References 35 publications

Sharka: The Past, The Present and The Future

Sharka: The Past, The Present and The Future

Unfolding the secrets of plum pox virus: from epidemiology to genomics

Turnip Mosaic Virus Uses the SNARE Protein VTI11 in an Unconventional Route for Replication Vesicle Trafficking

Contact Info

Product

Resources

About