Unfolding the secrets of plum pox virus: from epidemiology to genomics

Šubr, Z; Glasa, Miroslav

doi:10.4149/av_2013_02_217

Cited by 29 publications

(19 citation statements)

References 109 publications

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“…The PPV RNA is translated into a large self-cleaving polyprotein encoding 11 mature proteins (48, 130,136). After infecting the first mesophyll cells, the virus is able to move locally from cell to cell through plasmodesmata.…”

Section: Sharka Identity Cardmentioning

confidence: 99%

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Sharka Epidemiology and Worldwide Management Strategies: Learning Lessons to Optimize Disease Control in Perennial Plants

Rimbaud

Dallot

Gottwald

et al. 2015

Annu. Rev. Phytopathol.

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Many plant epidemics that cause major economic losses cannot be controlled with pesticides. Among them, sharka epidemics severely affect prunus trees worldwide. Its causal agent, Plum pox virus (PPV; genus Potyvirus), has been classified as a quarantine pathogen in numerous countries. As a result, various management strategies have been implemented in different regions of the world, depending on the epidemiological context and on the objective (i.e., eradication, suppression, containment, or resilience). These strategies have exploited virus-free planting material, varietal improvement, surveillance and removal of trees in orchards, and statistical models. Variations on these management options lead to contrasted outcomes, from successful eradication to widespread presence of PPV in orchards. Here, we present management strategies in the light of sharka epidemiology to gain insights from this worldwide experience. Although focused on sharka, this review highlights more general levers and promising approaches to optimize disease control in perennial plants.

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Section: Sharka Identity Cardmentioning

confidence: 99%

“…Thus, various alternative management strategies had to be implemented against sharka. Even though the PPV pathosystem has been well studied (for reviews, see 47, 48, 66,82,130,135,136), the epidemiological grounds underlying sharka management strategies have never been…”

Section: Introductionmentioning

confidence: 99%

Sharka Epidemiology and Worldwide Management Strategies: Learning Lessons to Optimize Disease Control in Perennial Plants

Rimbaud

Dallot

Gottwald

et al. 2015

Annu. Rev. Phytopathol.

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“…PPV is a member of the genus Potyvirus which, in nature, infects Prunus trees, although it can also infect experimental herbaceous hosts Subr and Glasa, 2013). The potyvirus genome is a positive-sense, single-stranded RNA of 10 kb encapsidated by a single type of CP to form flexuous rod particles.…”

Section: Introductionmentioning

confidence: 99%

Phosphorylation coexists with O‐GlcNAcylation in a plant virus protein and influences viral infection

Martínez-Turiño

Pérez

Hervás

et al. 2018

Molecular Plant Pathology

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Phosphorylation and O-GlcNAcylation are two widespread post-translational modifications (PTMs), often affecting the same eukaryotic target protein. Plum pox virus (PPV) is a member of the genus Potyvirus which infects a wide range of plant species. O-GlcNAcylation of the capsid protein (CP) of PPV has been studied extensively, and some evidence of CP phosphorylation has also been reported. Here, we use proteomics analyses to demonstrate that PPV CP is phosphorylated in vivo at the N-terminus and the beginning of the core region. In contrast with the 'yin-yang' mechanism that applies to some mammalian proteins, PPV CP phosphorylation affects residues different from those that are O-GlcNAcylated (serines Ser-25, Ser-81, Ser-101 and Ser-118). Our findings show that PPV CP can be concurrently phosphorylated and O-GlcNAcylated at nearby residues. However, an analysis using a differential proteomics strategy based on iTRAQ (isobaric tags for relative and absolute quantitation) showed a significant enhancement of phosphorylation at Ser-25 in virions recovered from O-GlcNAcylation-deficient plants, suggesting that crosstalk between O-GlcNAcylation and phosphorylation in PPV CP takes place. Although the preclusion of phosphorylation at the four identified phosphotarget sites only had a limited impact on viral infection, the mimicking of phosphorylation prevents PPV infection in Prunus persica and weakens infection in Nicotiana benthamiana and other herbaceous hosts, prompting the emergence of potentially compensatory second mutations. We postulate that the joint action of phosphorylation and O-GlcNAcylation in the N-proximal segment of CP allows a fine-tuning of protein stability, providing the amount of CP required in each step of viral infection.

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“…At its 5′-end, the RNA is linked to the viral genome-linked protein VPg, and the 3′-end carries a poly-A tail. The genome codes for a large polyprotein and a truncated frameshift product that are processed by three self-encoded proteases into at least 11 proteins [7], [8].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis of Prunus domestica Undergoing Hypersensitive Response to Plum Pox Virus Infection

et al. 2014

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Plum pox virus (PPV) infects Prunus trees around the globe, posing serious fruit production problems and causing severe economic losses. One variety of Prunus domestica, named ‘Jojo’, develops a hypersensitive response to viral infection. Here we compared infected and non-infected samples using next-generation RNA sequencing to characterize the genetic complexity of the viral population in infected samples and to identify genes involved in development of the resistance response. Analysis of viral reads from the infected samples allowed reconstruction of a PPV-D consensus sequence. De novo reconstruction showed a second viral isolate of the PPV-Rec strain. RNA-seq analysis of PPV-infected ‘Jojo’ trees identified 2,234 and 786 unigenes that were significantly up- or downregulated, respectively (false discovery rate; FDR≤0.01). Expression of genes associated with defense was generally enhanced, while expression of those related to photosynthesis was repressed. Of the total of 3,020 differentially expressed unigenes, 154 were characterized as potential resistance genes, 10 of which were included in the NBS-LRR type. Given their possible role in plant defense, we selected 75 additional unigenes as candidates for further study. The combination of next-generation sequencing and a Prunus variety that develops a hypersensitive response to PPV infection provided an opportunity to study the factors involved in this plant defense mechanism. Transcriptomic analysis presented an overview of the changes that occur during PPV infection as a whole, and identified candidates suitable for further functional characterization.

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Unfolding the secrets of plum pox virus: from epidemiology to genomics

Cited by 29 publications

References 109 publications

Sharka Epidemiology and Worldwide Management Strategies: Learning Lessons to Optimize Disease Control in Perennial Plants

Sharka Epidemiology and Worldwide Management Strategies: Learning Lessons to Optimize Disease Control in Perennial Plants

Phosphorylation coexists with O‐GlcNAcylation in a plant virus protein and influences viral infection

Transcriptomic Analysis of Prunus domestica Undergoing Hypersensitive Response to Plum Pox Virus Infection

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