c-Fos expression identifies brain areas activated in response to nortriptyline

Ribeiro, Marcos Borges; Oliveira, Irismar Reis de; Santana, Rejane Conceição; Costa, Daniel; Silva, E de Castro e; Fregoneze, J.B.

doi:10.1038/sj.mp.4001989

Cited by 3 publications

(3 citation statements)

References 9 publications

(20 reference statements)

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“…Administration of nortriptyline and escitalopram, without TST, had different effects on c-Fos expression in the brain. The effect of nortriptyline, which induced a significant c-Fos response within the central extended amygdala, hypothalamus, and brainstem, was in good accordance with previous studies [ 26 , 27 ]. In contrast, escitalopram induced no significant changes in c-Fos density in all regions examined.…”

Section: Discussionsupporting

confidence: 92%

Pattern of c-Fos expression induced by tail suspension test in the mouse brain

Hiraoka

Motomura

Yanagida

et al. 2017

Heliyon

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The tail suspension test (TST) has been widely used as a screening assay for antidepressant drugs. However, the neural substrates underlying the stress response and antidepressant-like effect during the TST remain largely unknown despite the prevalence of this test. In the present study, we used immunohistochemistry to examine alterations in c-Fos expression as a measure of neuronal activity in the mouse brain after acute administration of the antidepressant drugs nortriptyline or escitalopram (or saline as a control) with or without a subsequent TST session. We found that without the TST session, nortriptyline administration enhanced the density of c-Fos-immunoreactive cells in regions of the central extended amygdala, paraventricular hypothalamic nucleus, and relevant regions of the brain stem, whereas escitalopram did not change c-Fos expression in any region. Following the TST in the absence of antidepressant drugs, we observed a significant increase in c-Fos-positive cell density in a number of brain regions within the limbic telencephalon, hypothalamus, and brain stem. We detected a statistically significant interaction using an analysis of variance between the main effects of the drug and stress response in four regions: the infralimbic cortex, lateral septal nucleus (intermediate part), ventrolateral preoptic nucleus, and solitary nucleus. Following the TST, escitalopram but not nortriptyline increased c-Fos-positive cell density in the infralimbic cortex and ventrolateral preoptic nucleus, whereas nortriptyline but not escitalopram increased c-Fos expression in the solitary nucleus. Both antidepressants significantly increased c-Fos expression in the lateral septal nucleus (intermediate part). The present results indicate that neuronal activity increases in septo-hypothalamic regions and related structures, especially the lateral septal nucleus, following administration of drugs producing an antidepressant-like effect in mice subjected to the TST.

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Section: Discussionsupporting

confidence: 92%

Pattern of c-Fos expression induced by tail suspension test in the mouse brain

Hiraoka

Motomura

Yanagida

et al. 2017

Heliyon

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“…Homeostatic pressures, such as those produced by reduced energy reserves, however, are not SCN‐regulated nor do such homeostatic cues affect the phase of SCN rhythms. To explore the mechanisms underlying arousal in response to homeostatic food‐related pressures, we designed a study in which arousal‐related cues produced by the circadian system and the homeostatic effects consequent to depleted energy stores were pitted against each other 80 . We measured the neuronal activation in 16 different brain regions that have been implicated in food anticipatory activity (FAA) or in eating, using c‐fos immunochemistry to determine which neurons were the first to be activated in mice exhibiting arousal in anticipation of feeding restricted to their inactive phase.…”

Section: Homeostatic and Circadian Timing Mechanisms In Sleep And Feementioning

confidence: 99%

“…To explore the mechanisms underlying arousal in response to homeostatic food-related pressures, we designed a study in which arousal-related cues produced by the circadian system and the homeostatic effects consequent to depleted energy stores were pitted against each other. 80 We measured the neuronal activation in 16 different brain regions that have been implicated in food anticipatory activity (FAA) or in eating, using c-fos immunochemistry to determine which neurons were the first to be activated in mice exhibiting arousal in anticipation of feeding restricted to their inactive phase. The results indicate that the number of c-FOS-positive neurons was significantly increased in only one of the brain regions studied-the ventromedial nucleus of the hypothalamus (VMH).…”

Section: Relationship Of Feo To Leomentioning

confidence: 99%

Circadian and Homeostatic Factors in Arousal

Silver

LeSauter

2008

Annals of the New York Academy of Sciences

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In the course of evolution, mechanisms have evolved to anticipate the timing of regularly occurring events. These mechanisms are encompassed in a circadian timing system that include a master clock localized to the suprachiasmatic nucleus of the hypothalamus and "slave" oscillators distributed throughout the body. This system serves multiple functions so as to ensure that various physiological processes occur at optimal and nonoverlapping times, to synchronize our activities to local environmental time, and to permit changes required to respond to new environmental circumstances. We suggest that a generalized concept of arousal (which includes alterations in responsiveness to homeostatic pressures, sensory stimuli and emotional reactivity, and to changes in motor activity) serves as a rubric in which to explore the multiple ways in which the circadian system modulates behavior.

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Acute Impact of Selected Pyridoindole Derivatives on Fos Expression in Different Structures of the Rat Brain

et al. 2017

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The impacts of three pyridoindole derivatives (PDs), designated as PD144, PD143, and PD104, which have previously been shown to have antidepressant (PD144) and anxiolytic (PD143, PD104) properties, were investigated on the Fos expressions in 11 different rat brain areas, including the medial prefrontal cortex, striatum, septum, accumbens nucleus (shell, core), bed nucleus of the stria terminalis, hypothalamic paraventricular nucleus, central amygdala, locus coeruleus, dorsal raphe nucleus, and the solitary tract nucleus. Control rats received vehicle, while the other three groups the PDs in a dose of 25 mg/kg/b.w. The animals were transcardially perfused with a fixative 90 min after the treatments. Coronal sections of 40-µm thickness were processed for Fos-immunostaining by avidin-biotin-peroxidase complex and visualized by nickel-intensified diaminobenzidine complex. Fos-labeled sections were counterstained with neuropeptides including corticoliberine (CRH), oxytocin (OXY), vasopressin (AVP), and vasoactive intestinal polypeptide (VIP) and processed for immunofluorescence staining using Alexa Fluor 555 dye. In all the three groups of animals, the upregulation of PDs-induced Fos expression only in 2 of 11 brain areas was investigated, namely, in the hypothalamic paraventricular nucleus (PVN) and the central amygdaloid nucleus (CeA). The other brain structures studied were devoid of Fos expression. Counterstaining of the Fos-labeled CeA-containing sections with VIP antibody revealed that the Fos expression stimulated by the PDs was upregulated in all the CeA subdivisions (lateral, ventral, capsular), except the medial one. Dual immunoprocessings showed Fos/CRH-labeling in both the PVN and the amygdala and Fos/OXY in the PVN. No Fos/AVP colocalizations were seen in the PVN. The obtained data provide the first view on the intracerebral effects of three new PDs derivatives, which effects were restricted only to the PVN and CeA areas. The present data may help to improve our understanding of the impact of the selected PDs on the brain and to anticipate possible behavioral and neuroendocrine consequences.

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c-Fos expression identifies brain areas activated in response to nortriptyline

Cited by 3 publications

References 9 publications

Pattern of c-Fos expression induced by tail suspension test in the mouse brain

Pattern of c-Fos expression induced by tail suspension test in the mouse brain

Circadian and Homeostatic Factors in Arousal

Acute Impact of Selected Pyridoindole Derivatives on Fos Expression in Different Structures of the Rat Brain

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