Burkholderia nodosa sp. nov., isolated from root nodules of the woody Brazilian legumes Mimosa bimucronata and Mimosa scabrella

Abstract

T , Br3461 and Br3470, were isolated from nitrogen-fixing nodules on the roots of Mimosa scabrella (Br3437 T ) and Mimosa bimucronata (Br3461, Br3470), both of which are woody legumes native to Brazil. On the basis of 16S rRNA gene sequence similarities, all the strains were shown previously to belong to the genus Burkholderia. A polyphasic approach, including DNA-DNA hybridizations, PFGE of whole-genome DNA profiles, whole-cell protein analyses, fatty acid methyl ester analysis and extensive biochemical characterization, was used to clarify the taxonomic position of these strains further; the strains are here classified within a novel species, for which the name Burkholderia nodosa sp. nov. is proposed. The type strain, Br3437 T and NGR195A (isolated, respectively, from Machaerium lunatum in French Guiana and Mimosa invisa in New Guinea; Moulin et al., 2001;Vandamme et al., 2002;Elliott et al., 2007) and various strains of Burkholderia mimosarum (isolated from M. pigra in Taiwan and Brazil;Chen et al., 2006), the taxonomic positions of most Burkholderia legume symbionts have not yet been described. The aim of the present study is to clarify the taxonomic positions of three strains isolated fromThe GenBank/EMBL/DDBJ accession numbers for 16S rRNA gene sequences of strains Br3437 T , Br3470 and Br3461 are respectively AY773189 and AM284971, AY773198 and AM284972, and AY533861 and AM284970 (two determinations for each strain).An extended phylogenetic tree and details of genome sizes, DNA-DNA binding values and fatty acid compositions of the novel strains and

Novel Burkholderia bacteria isolated from Lebeckia ambigua – A perennial suffrutescent legume of the fynbos

“…Currently, six Burkholderia species, including B. mimosarum (Chen et al, 2006), B. nodosa (Chen et al, 2007), B. phymatum (Vandamme et al, 2002), B. sabiae (Chen et al, 2008), B. symbiotica (Sheu et al, 2012) and B. tuberum (Vandamme et al, 2002) have been confirmed to nodulate and fix nitrogen in symbiosis with legumes. Although sharing the ability to nodulate they are dispersed throughout the 16S rRNA gene phylogenetic tree (Figs.…”

Bukholderia strains promote Mimosa spp. growth but not Macroptilium atropurpureum

“…This affinity was indicated by the high frequency of Burkholderia strains isolated from Mimosa spp. located in different ecosystems (BARRET; PARKER, 2005;BONTEMPS et al, 2010;CHEN et al, 2003CHEN et al, , 2005CHEN et al, , 2006CHEN et al, , 2007CHEN et al, , 2008MISHRA et al, 2012;REIS JÚNIOR et al, 2010). However, Elliot et al (2007) found that the strain Burkholderia tuberum STM678…”

Suppression of Bacterial Wilt and Fusarium Wilt by a <i>Burkholderia nodosa</i> Strain Isolated from Kalimantan Soils, Indonesia

“…1). C17:0 cyclo is major fatty acid in B. ferrariae 38 , but not in B. nodosa 6) . Thus, the fatty acid profile of strain G5.2 was considered to be more similar to that of B. nodosa.…”

“…We checked by 16S rDNA sequences in 4 species but we did not sequence one isolate (G4.2), because in term of colony and substrate utilization of G4.1 and G4.2 isolates were similar. Fatty acid methyl ester profiles of strains C13.1, G4.1, G5.1 and G5.2 were compared with those of related Burkholderia species such us B. sacchari 4) , B. pyrrocinia 32) , B. terricola 11) , B. nodosa 6) , B. ferrariae 38) , B. unamae 5) and B. kururiensis 44) . The fatty acid profiles of strains C13.1, G4.1 and G5.1 were similar to those of B. sacchari, B. pyrrocinia and B. terricola, respectively.…”