Bulk isolation of immune response-related genes by expressed sequenced tags of Japanese flounder leucocytes stimulated with Con A/PMA

Expression analysis of immune-relevant genes in the spleen of large yellow croaker (Pseudosciaena crocea) stimulated with poly I:C

“…As a compensation of genomic resources, EST analysis is a powerful approach for identifying new genes and profiling gene expression in tissues and cells. In regard to this, EST resources of these model fish and other fish with great economical interests such as Japanese flounder [6,7], Atlantic salmon [8], rainbow trout [9], and red seabream [10] have grown quickly in recent years. Many immune-relevant genes have been successfully identified in bony fish by EST analysis.…”

Expressed sequence tags analysis of Atlantic halibut (Hippoglossus hippoglossus) liver, kidney and spleen tissues following vaccination against Vibrio anguillarum and Aeromonas salmonicida

“…Several of these studies were specifically designed to identify genes involved in innate and acquired immunity. These studies include the EST studies on Japanese flounder leucocytes infected with Hirame rhabdovirus [11,12], Japanese flounder leucocytes stimulated with Con A/PMA [14] and Japanese flounder kidney tissues from peptidoglycan-stimulated fish [13]. In addition, ESTs have been obtained from kidney and gill tissues of rainbow trout infected with infectious hematopoietic necrosis virus (IHNV) and from head kidney cells of common carp after stimulation with lipopolysaccharide and concanavalin A [18,20].…”

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“…The expression of Hsp90a gene was studied in a chinook salmon embryonic cell line and it was shown to be heat inducible (Palmisano et al, 2000). A fragment of Hsp90a has been cloned from the Japanese flounder (Paralichtys olivaceus) (Nam et al, 2003). An hsp90 sequence from Atlantic salmon (Salmo salar) was characterized by Pan et al (2000) which corresponded to the Hsp90b of zebrafish with a 92% amino acid identity.…”