Expression analysis of immune-relevant genes in the spleen of large yellow croaker (Pseudosciaena crocea) stimulated with poly I:C

Feeding common carp Cyprinus carpio with β-glucan supplemented diet stimulates C-reactive protein and complement immune acute phase responses following PAMPs injection

“…This suggests that poly(I:C) injection had an effect on complement response in carp and this effect was organ and time dependent. Although information about poly(I:C) effects on acute phase protein profiles in fish is limited, these results are in accordance with previous studies performed on Pseudosciaena crocea [52,53] and S. salar [54] where poly(I:C) induced an acute phase profile in the expression of related genes. Regarding b-glucan fed fish, poly(I:C) did not have an effect on serum acute phase protein levels.…”

Identification and characterization of interferon regulatory factor-1 from orange-spotted grouper (Epinephelus coioides)

“…PolyI:C is a synthetic molecule mimicking viral double-stranded RNA [32]. Many reports have demonstrated that polyI:C induces the expression of immune-related genes [33][34][35][36]. It is assumed that the different IRF-1 expression level is one of the factors that dictate how it functions.…”

Characterization of a RacGTPase up-regulated in the large yellow croaker Pseudosciaena crocea immunity

“…Based on the fragment of the Rac gene of large yellow croaker obtained in previous study (EST, accession number CX348583) [22], 3 0 RACEs was done using 3 0 RACE Kit (Promega, USA) to amplify the 3 0 UTR of LycRac gene according to the manufacturer's instructions. The 3 0 end RACE PCR reaction was performed using the gene-specific primer LycRac-F1 (5 0 -TGTCTTCTCATCAGCTAC-3 0 ) and the adapter primer AOLP.…”