Building An Aptamer/Graphene Oxide FRET Biosensor for One-Step Detection of Bisphenol A

Abstract

Bisphenol A (BPA) is an important industrial chemical for polycarbonate (PC) and epoxy resins in paper and plastic industries. In our work, a kind of new method for detection of BPA was designed based on graphene oxide and anti-BPA aptamer. The graphene oxide can specifically adsorb and quench the fluorescence of fluorescently modified ssDNA probes. Meanwhile, the BPA can combine with anti-BPA optamer and switch its configuration to prevent the aptamer from adsorbing on the surface of graphene oxide (GO). Under different concentrations of BPA, based on the target-induced conformational change of anti-BPA aptamer and the interactions between the fluorescently modified anti-BPA aptamer (FAM-ssDNA) and GO, the experimental results show that the intensity of the fluorescence signal was changed. A low limit of detection of 0.05 ng/mL was obtained in the range 0.1-10 ng/mL. In addition, the specificity was outstanding among analogues of BPA. The recovery rate in actual water samples spiked with BPA can be 96.0% to 104.5%. The developed method was successfully used to determine BPA in actual water samples.

A signal-enhanced lateral flow strip biosensor for ultrasensitive and on-site detection of bisphenol A

“…Enzyme-linked immunosorbent assay was the initial method of choice (Lei et al, 2013;Maiolini et al, 2014), and with the advent of electrochemical biosensors, an electrochemical immunosensor for BPA with "signal-on" detection based on square-wave voltammetry was fabricated (Wang et al, 2013). Besides, aptamer have received considerable attention as recognition probes in bioassays, and methods for the trace detection of BPA based on fluorescence resonance energy transfer (Zhu et al, 2015) and a label-free aptasensor based on colorimetric detection have been developed. In addition, an electrochemical aptasensor for screening drinking water for the presence of BPA has also been reported .…”

Nanomaterial based aptasensors for clinical and environmental diagnostic applications

“…Addition of target molecules to the system detaches the strands from GO restoring the uorescence leading to an increase in the signal. While the work of Zhu & co-workers applied a more conventional approach of using graphene oxide (GO) as a quencher in a green emissive uorophore modied aptamer, 73 the work of Xu et al & Ling et al revolved around a more novel "turning-on" signal, in an intricate play between the aptamer and its complementary uorophore-labelled helper DNA. 74,75 While their interaction in control samples keeps the helper DNA from interacting with nanosheets, the introduction of target molecules displaces the helper DNA from aptamers leading to their absorption on the surface of nanosheets causing it to quench (Fig.…”

Nanoparticles as energy donors and acceptors in bionanohybrid systems

“…Hence, graphene oxide nanoparticles are extensively investigated as FRET quenchers in pair with adsorbed ssDNA fluorophore-labeled probes. As FRET bionanosensors, where detection is based on the cleavage or desorption of ssDNA and the increase of fluorescent signal, they were employed for exonuclease activity quantification (Liu et al, 2019), copper ions sensing by DNAzyme autocatalysis (Ding et al, 2018) or aptamer-dependent detection of toxins (Zhu et al, 2015).…”