Buccal micronucleus cytome assay in primary school children: A descriptive analysis of the MAPEC_LIFE multicenter cohort study

Villarini, Milena; Levorato, Sara; Salvatori, Tania; Ceretti, Elisabetta; Bonetta, Sara; Carducci, Annalaura; Grassi, Tiziana; Vannini, Samuele; Donato, Francesco; Bonetta, Silvia; Verani, Marco; Donno, Antonella De; Bonizzoni, Silvia; Bonetti, A.; Moretti, Massimo; Gelatti, Umberto; Fatigoni, Cristina; Monarca, Silvano; Covolo, Loredana; Feretti, Donatella; Festa, Andrea; Viola, Gaia Claudia Viviana; Zani, Claudia; Zerbini, Ilaria; Gilli, Giorgio; Carraro, Elisabetta; Schilirò, Tiziana; Pignata, Cristina; Gea, Marta; Romanazzi, Valeria; Donzelli, Gabriele; Palomba, Giacomo; Bagordo, Francesco; Giorgi, Maria Grazia De; Guido, Marcello; Idolo, Adele; Panìco, Alessandra; Serio, Francesca; Verri, Tiziano; Furia, C.; Colombi, Paolo; Codenotti, R.; Zagni, Licia

doi:10.1016/j.ijheh.2018.05.014

Cited by 30 publications

(29 citation statements)

References 44 publications

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“…Moreover, a group of Brazilian children aged ≤7 years living in an urban polluted area showed a mean MNC frequency of 1.20 ± 0.83 MNC‰ while those living in a rural nonpolluted area measured 0.19 ± 0.31 MNC‰ [45]. Finally, the level of MNC detected in the IMP.AIR study appeared consistent with that reported during the winter in some Italian cities with high urban air pollution such as Torino (0.39 ± 0.48 MNC‰) and Brescia (0.53 ± 0.61 MNC‰) [33]. In summary, from an environmental point of view, the frequency of biomarkers found in this study appeared to be non-basic and similar to the level recorded in some polluted areas.…”

Section: Discussionsupporting

confidence: 73%

“…In this study, the frequency of MNC was significantly lower (0.24 ± 0.32 MNC‰) [33], suggesting that children living in the cluster area are more likely to have early DNA damage than children living outside of it. As previously stated [16,18,19,42], this may imply an increased risk to develop cancer in adulthood.…”

Section: Discussionmentioning

confidence: 90%

“…Results from analysis performed on BMC of 213 children living in Lecce and included in the cohort of MAPEC_LIFE study [33] were also reported. In the IMP.AIR study MNC were found in 39 (41.5%) samples, NBUDs in 8 (8.5%) samples, BC in 66 (70.2%) samples, BNC in 88 (93.6%) samples, KHC in 91 (96.8%) samples and PYK in 37 (39.4%) samples.…”

Section: Resultsmentioning

confidence: 99%

“…Finally, a comparison between the results concerning the early DNA damage in children recruited in the IMP.AIR study and those detected in children recruited in an area of Salento not included in the cluster area [33] was performed. The reference cohort was the one recruited in the MAPEC_LIFE study [34], which includes 213 children aged 6–8 years living in Lecce.…”

Section: Methodsmentioning

confidence: 99%

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Micronuclei in Exfoliated Buccal Cells of Children Living in a Cluster Area of Salento (Southern Italy) with a High Incidence of Lung Cancer: The IMP.AIR Study

Idolo

Grassi

Bagordo

et al. 2018

IJERPH

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During the years 2014–2016 the University of Salento performed the “Impact of Air Quality on Health of Residents in the Municipalities of Cutrofiano, Galatina, Sogliano Cavour, Soleto and Sternatia” (IMP.AIR) study, an epidemiological-molecular research project aiming to evaluate early DNA damage in children living in an area of Salento with high incidence of lung cancer among the male population. One hundred and twenty-two children aged 6–8 years attending primary school were enrolled and the frequency of micronucleated cells (MNC) in oral mucosa was evaluated. In addition, a questionnaire was administered to parents to obtain information about personal data, anthropometric characteristics and lifestyles (physical activity, food habits, family context) of the children and perform a multivariate analysis to detect any factors associated with MNC occurrence. Data on airborne pollutants detected in the study area were acquired by the Regional Agency for the Environmental Protection. The presence of MNC was highlighted in about 42% of children with a mean MNC frequency of 0.49‰. The frequency of MNC was associated to obesity, consumption of red or processed meat and having a mother who smokes. Moreover, the prevalence of biomarkers was higher than in another area of Salento not included in the cluster area.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Micronuclei in Exfoliated Buccal Cells of Children Living in a Cluster Area of Salento (Southern Italy) with a High Incidence of Lung Cancer: The IMP.AIR Study

Idolo

Grassi

Bagordo

et al. 2018

IJERPH

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“…It aimed to evaluate, both by toxicological and epidemiologic-molecular approaches, the association between air pollution exposure and early biological effect in 6–8-year-old children living in Italian areas with varying levels of air pollution [24,25]. …”

Section: Methodsmentioning

confidence: 99%

Health Risk Associated with Exposure to PM10 and Benzene in Three Italian Towns

Donno

Giorgi

Bagordo

et al. 2018

IJERPH

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Air pollution in urban areas is a major concern as it negatively affects the health of a large number of people. The purpose of this study was to assess the inhalation health risk for exposure to PM10 and benzene of the populations living in three Italian cities. Data regarding PM10 and benzene daily measured by “traffic” stations and “background” stations in Torino, Perugia, and Lecce during 2014 and 2015 were compared to the limits indicated in the Directive 2008/50/EC. In addition, an inhalation risk analysis for exposure to benzene was performed for adults and children by applying the standard United States Environmental Protection Agency’s (USEPA) methodology. The levels of PM10 detected in Torino exceeded the legal limits in both years with an increased mean concentration >10 µg/m3 comparing with background station. Benzene concentrations never exceeded the legislative target value. The increased cancer risk (ICR) for children exposed to benzene was greater than 1 × 10−6 only in the city of Torino, while for adults, the ICR was higher than 1 × 10−6 in all the cities. The results suggest the need for emission reduction policies to preserve human health from continuous and long exposure to air pollutants. A revision of legal limits would also be recommended.

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Monitoring human genotoxicity risk associated to urban and industrial Buenos Aires air pollution exposure

Gutiérrez

Palmieri

Giuliani

et al. 2020

Environ Sci Pollut Res

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The quality of life in large megacities is directly affected by its air quality. In urban environments, suspended particles from anthropogenic origin is one of the main air contaminants identified as highly genotoxic, mutagenic, or carcinogenic. Atmospheric monitoring is therefore imperative, and bioassays to detect the effects of genotoxic agents give usually excellent results. Analysis of micronucleus (MN) in exfoliated oral mucosa cells is a sensitive non-invasive method for monitoring genetic damage in human populations. The first aim of this study was to analyze and characterize levels of volatile organic compounds (VOCs), particulate matter (PM), and polycyclic aromatic hydrocarbons (PAHs) in two areas from Buenos Aires: La Plata city, an urban (U) area and Ensenada, an industrial (I) area. Secondly, we evaluated the possible health risk of its inhabitants through a simple genotoxic assay on exfoliated oral mucosa cells. Whole blood cell count and nuclear abnormalities frequencies were evaluated in the exfoliated oral mucosa cells from urban and industrial inhabitants. Smoking habit represented a significant factor increasing MN percentage while, age did not increase the production of any of the nuclear aberrations assayed (micronuclei, binucleated, karyorrhexis) when the inhabitants from the urban and the industrial areas were compared. In addition, changes in MN and binucleated cell percentages in males and females were found to be area-dependent. We suggest that regardless PM concentration, PM-specific characteristics (size, shape, chemical elements, etc.) and VOCs levels could be responsible for the different harmful genotoxic effects seen in the two areas. Although this is a preliminary study, our results allowed to recognize that individuals living in both the urban and the industrial areas could be considered susceptible groups and should periodically undergo biological monitoring and appropriate care.

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Buccal micronucleus cytome assay in primary school children: A descriptive analysis of the MAPEC_LIFE multicenter cohort study

Cited by 30 publications

References 44 publications

Micronuclei in Exfoliated Buccal Cells of Children Living in a Cluster Area of Salento (Southern Italy) with a High Incidence of Lung Cancer: The IMP.AIR Study

Micronuclei in Exfoliated Buccal Cells of Children Living in a Cluster Area of Salento (Southern Italy) with a High Incidence of Lung Cancer: The IMP.AIR Study

Health Risk Associated with Exposure to PM10 and Benzene in Three Italian Towns

Monitoring human genotoxicity risk associated to urban and industrial Buenos Aires air pollution exposure

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