Buccal Fat Pad as a Potential Source of Stem Cells for Bone Regeneration: A Literature Review

Salehi, Asiyeh; Rad, Maryam Rezai; Kheiri, Lida; Nazeman, Pantea; Nadjmi, Nasser; Khojasteh, Arash

doi:10.1155/2017/8354640

Cited by 43 publications

(36 citation statements)

References 88 publications

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“…The BFP approach obtains adipose tissue via the oral cavity and does not injure the external facial surface of the patient and thus provides an esthetic advantage. Since BFP is retained regardless of the weight of the patient, it can also be collected from patients who are thin and have less subcutaneous fat (Kishimoto et al., ; Salehi‐Nik et al., ). Therefore, the BFP approach is much less invasive and more beneficial than harvesting DFAT cells from subcutaneous adipose tissue.…”

Section: Human Dfat Cells From the Buccal Fat Pad (Bfp)—a Clinically mentioning

confidence: 99%

Dedifferentiated Fat (DFAT) cells: A cell source for oral and maxillofacial tissue engineering

et al. 2018

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Tissue engineering is a promising method for the regeneration of oral and maxillofacial tissues. Proper selection of a cell source is important for the desired application. This review describes the discovery and usefulness of dedifferentiated fat (DFAT) cells as a cell source for tissue engineering. Dedifferentiated Fat cells are a highly homogeneous cell population (high purity), highly proliferative, and possess a multilineage potential for differentiation into various cell types under proper in vitro inducing conditions and in vivo. Moreover, DFAT cells have a higher differentiation capability of becoming osteoblasts, chondrocytes, and adipocytes than do bone marrow-derived mesenchymal stem cells and/or adipose tissue-derived stem cells. The usefulness of DFAT cells in vivo for periodontal tissue, bone, peripheral nerve, muscle, cartilage, and fat tissue regeneration was reported. Dedifferentiated Fat cells obtained from the human buccal fat pad (BFP) are a minimally invasive procedure with limited esthetic complications for patients. The BFP is a convenient and accessible anatomical site to harvest DFAT cells for dentists and oral surgeons, and thus is a promising cell source for oral and maxillofacial tissue engineering.

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Section: Human Dfat Cells From the Buccal Fat Pad (Bfp)—a Clinically mentioning

confidence: 99%

Dedifferentiated Fat (DFAT) cells: A cell source for oral and maxillofacial tissue engineering

et al. 2018

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“…Studies have shown a great differentiation potential of BFP-derived mesenchymal stem cells (BFP-MSCs) under convenient situation into the adipocytes, chondrocytes, and osteoblasts in vitro and in vivo. 31,32 The aim of the current study was to introduce a new composite nanofibrous scaffold for simultaneous delivery of the Cur and BFP-MSCs. After a literature review, we decided to use hydrophilic polymers such as CS and carbopol as materials of scaffolding and PVA and polycaprolactone (PCL) to improve the manipulating of the polymers for acquiring good fibers.…”

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confidence: 99%

Biological behavior of the curcumin incorporated chitosan/poly(vinyl alcohol) nanofibers for biomedical applications

Golchin

Hosseinzadeh

Staji

et al. 2019

J of Cellular Biochemistry

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Electrospun composite scaffolds show high ability to be used in regenerative medicine and drug delivery, due to the nanofibrous structure and high surface area to volume ratio. In this study, we used nanofibrous scaffolds fabricated by chitosan (CS), poly(vinyl alcohol) (PVA), carbopol, and polycaprolactone using a dual electrospinning technique while curcumin (Cur) incorporated inside of the CS/PVA fibers. Scaffolds were fully characterized via scanning electron microscopy, water contact angle, tensile measurement, hydration, protein adsorption, and wrinkled tests. Furthermore, viability of the buccal fat pad‐derived mesenchymal stem cells (BFP‐MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds. Cell cycle assay was also performed to more detailed evaluation of the stem cells growth when grown on scaffolds (with and without Cur) compared with the culture plate. Results demonstrated that Cur loaded nanofibrous scaffold had more suitable capability for water absorption and mechanical properties compared with the scaffold without Cur and it could also support the stem cells viability and proliferation. Cur release profile showed a decreasing effect on BFP‐MSCs viability in the initial stage, but it showed a positive effect on stem cell viability in a long‐term manner. In general, the results indicated that this nanofibrous scaffold has great potential as a delivery of the Cur and BFP‐MSCs simultaneously, and so holds the promising potential for use in various regenerative medicine applications.

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“…Adipose tissue can provide higher yield of stem cells, compared with bone marrow aspiration, thereby offering an advantage as an alternative source of MSCs . BFPSCs in comparison to ADSCs from other sources have shown similar efficacy in differentiation and adherence to collagen membrane and polyglycolic acid . Moreover, similar expression level of RUNX‐2, osteopontin, osteocalcin, and ALP activity have been detected in BFPSCs, compared to bone marrow MSC .…”

Section: Discussionmentioning

confidence: 98%

Buccal fat pad‐derived stem cells with anorganic bovine bone mineral scaffold for augmentation of atrophic posterior mandible: An exploratory prospective clinical study

Khojasteh

Hosseinpour

Rad

et al. 2019

Clin Implant Dent Rel Res

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Background Application of adipose‐derived stem cells originated from buccal fat pad (BFP) can simplify surgical procedures and diminish clinical risks compared to large autograft harvesting. Purpose This study sought to evaluate and compare the efficacy of buccal fat pad‐derived stem cells (BFPSCs) in combination with anorganic bovine bone mineral (ABBM) for vertical and horizontal augmentation of atrophic posterior mandibles. Materials and Methods Fourteen patients with atrophic posterior mandible were elected for this prospective exploratory study. BFP (3‐5 mL) was harvested and BFPSCs were isolated and combined with ABBM at 50% ratio. The vertical and horizontal alveolar deficiencies were augmented by 50% mixture of ABBM with either BFPSCs (group 1) or particulated autologous bone (group 2). Titanium mesh was contoured to the desired 3D shape of the alveolar ridge and fixated to the host sites over the graft material of the two groups. At first, the amount of new bone areas was calculated by quantitative analysis of cone beam computed tomography (CBCT) images that were taken 6 months postoperatively according to regenerative techniques (group 1 vs group 2 without considering the type of bone defects). Second, these amounts were calculated in each group based on the type of defects. Results Quantitative analysis of CBCT images revealed the areas of new bone formation were 169.5 ± 5.90 mm2 and 166.75 ± 10.05 mm2 in groups 1 and 2, respectively. The area of new bone formation for vertical defects were 164.91 ± 3.74 mm2 and 169.36 ± 12.09 mm2 in groups 1 and 2, respectively. The area of new bone formation for horizontal deficiencies were 170.51 ± 4.54 mm2 and 166.98 ± 9.36 mm2 in groups 1 and 2, respectively. There were no statistically significant differences between the two groups in any of the pair‐wise comparisons (P > 0.05). Conclusions The findings of the present study demonstrated lack of difference in bone volume formation between BFPSCs and autologous particulate bone in combination with ABBM. If confirmed by future large‐scale clinical trial, BFPSCs may provide an alternative to autogenous bone for reconstruction of alveolar ridge defects.

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Buccal Fat Pad as a Potential Source of Stem Cells for Bone Regeneration: A Literature Review

Cited by 43 publications

References 88 publications

Dedifferentiated Fat (DFAT) cells: A cell source for oral and maxillofacial tissue engineering

Dedifferentiated Fat (DFAT) cells: A cell source for oral and maxillofacial tissue engineering

Biological behavior of the curcumin incorporated chitosan/poly(vinyl alcohol) nanofibers for biomedical applications

Buccal fat pad‐derived stem cells with anorganic bovine bone mineral scaffold for augmentation of atrophic posterior mandible: An exploratory prospective clinical study

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