Biological behavior of the curcumin incorporated chitosan/poly(vinyl alcohol) nanofibers for biomedical applications

Abstract

Electrospun composite scaffolds show high ability to be used in regenerative medicine and drug delivery, due to the nanofibrous structure and high surface area to volume ratio. In this study, we used nanofibrous scaffolds fabricated by chitosan (CS), poly(vinyl alcohol) (PVA), carbopol, and polycaprolactone using a dual electrospinning technique while curcumin (Cur) incorporated inside of the CS/PVA fibers. Scaffolds were fully characterized via scanning electron microscopy, water contact angle, tensile measurement, hydration, protein adsorption, and wrinkled tests. Furthermore, viability of the buccal fat pad‐derived mesenchymal stem cells (BFP‐MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds. Cell cycle assay was also performed to more detailed evaluation of the stem cells growth when grown on scaffolds (with and without Cur) compared with the culture plate. Results demonstrated that Cur loaded nanofibrous scaffold had more suitable capability for water absorption and mechanical properties compared with the scaffold without Cur and it could also support the stem cells viability and proliferation. Cur release profile showed a decreasing effect on BFP‐MSCs viability in the initial stage, but it showed a positive effect on stem cell viability in a long‐term manner. In general, the results indicated that this nanofibrous scaffold has great potential as a delivery of the Cur and BFP‐MSCs simultaneously, and so holds the promising potential for use in various regenerative medicine applications.

Wound healing improvement by curcumin‐loaded electrospun nanofibers and BFP‐MSCs as a bioactive dressing

“…1,7‐Bis(4‐hydroxy‐3‐methoxyphenyl)‐1,6‐heptadiene‐3,5‐dione, which is recognized as Cur or diferuloylmethane, is included as one of the primary Curcuma longa components, and the other is Curcuma spp. rhizome . According to pharmaceutical characteristics, Cur has shown to have antibacterial, antifungal, antiviral, antioxidant, anti‐inflammatory, anticoagulant, antiatherosclerotic, hypoglycemic and anticarcinogenesis impacts .…”

“…rhizome . According to pharmaceutical characteristics, Cur has shown to have antibacterial, antifungal, antiviral, antioxidant, anti‐inflammatory, anticoagulant, antiatherosclerotic, hypoglycemic and anticarcinogenesis impacts . The main restriction for Cur is poor bioavailability and bioactivity that subsequently, in neutral pH, has a poor absorbability, insolubility features, higher metabolism level, inactive metabolic products, short half‐life time in plasma and fast liver and intestine clearance .…”

“…In many studies, Cur is loaded onto a synthesized polymer such as chitosan (CS), polyvinyl alcohol (PVA), poly(ε‐caprolactone) (PCL), poly(2‐hydroxyethyl methacrylate) (HEMA), poly( dl ‐lactic‐co‐glycolic) acid, poly(lactic acid), cellulose acetate and their copolymers and blends . CS is a biodegradable, biocompatible polymer regarded as safe for human dietary use and approved for wound‐dressing applications, and PVA is also a type of nontoxic, water‐soluble and biocompatible synthetic polymer . According to the literature review, we decided to use hydrophilic polymers such as CS and carbopol as materials of scaffolding and PVA and PCL to improve the manipulation of the polymers to acquire good fibers .…”

“…CS is a biodegradable, biocompatible polymer regarded as safe for human dietary use and approved for wound‐dressing applications, and PVA is also a type of nontoxic, water‐soluble and biocompatible synthetic polymer . According to the literature review, we decided to use hydrophilic polymers such as CS and carbopol as materials of scaffolding and PVA and PCL to improve the manipulation of the polymers to acquire good fibers . Therefore, these polymers are selected for the purpose of the present study.…”

“…The present study aimed to introduce a novel nanofibrous scaffold composite for simultaneous delivery of BFP‐MSCs and Cur in a full‐thickness wound of mouse model. Following our last study and fully characterizing our designed scaffold, we decided to use CS/PVA/Cur + Carbopol/PCL nanofibrous scaffolds as a BFP‐MSC delivery scaffold for evaluating wound healing in the mouse model (Figure A).…”