BUB1B promotes hepatocellular carcinoma progression via activation of the mTORC1 signaling pathway

Qiu, Jiannan; Zhang, Shaopeng; Wang, Peng; Wang, Hao; Sha, Bowen; Peng, Hao; Zheng, Jie; Rao, Jianhua; Lü, Ling

doi:10.1002/cam4.3411

Cited by 49 publications

(38 citation statements)

References 37 publications

(72 reference statements)

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“…BUB1 promotes the occurrence and development of HCC by activating mTORC1-related proteins (33). PLK1 and CDK1 are important molecules in the abnormal cell cycle of HCC cells (34).…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics Profiling of Five Immune-Related lncRNAs for a Prognostic Model of Hepatocellular Carcinoma

Wei

Liu

et al. 2021

Front. Oncol.

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Hepatocellular carcinoma (HCC), one of the most common tumors worldwide, has the fifth highest mortality rate, which is increasing every year. At present, many studies have revealed that immunotherapy has an important effect on many malignant tumors. The main purpose of our research was to verify and establish a new immune-related lncRNA model and to explore the potential immune mechanisms. We analysed the pathways and mechanisms of immune-related lncRNAs by bioinformatics analysis, screened key lncRNAs based on Cox regression analysis, and determined the characteristics of the immune-related lncRNAs. On this basis, a predictive model was established. Through a comparison of specificity and sensitivity, we found that the constructed model was superior to the known markers of HCC. Then, the cell types were identified by the relative subgroup (CIBERSORT) algorithm for RNA transcripts. A signature model was eventually constructed, and we proved that it was a survival factor for HCC. Moreover, five kinds of immune cells were significantly positively correlated with the signature. The results indicated that these five kinds of lncRNAs may be related to the immune infiltration of hepatocellular carcinoma. To verify these findings, we selected the top coexpressed lncRNA, AC099850.3, for further study. We found that AC099850.3 could promote the migration and proliferation of hepatocellular carcinoma cells in vitro. RT-PCR experiments found that AC099850.3 could promote the expression of the cell cycle molecules BUB1, CDK1, PLK1, and TTK, and western blotting to prove that the expression of the molecules CD155 and PD-L1 was inhibited in the interference group. In conclusion, we used five kinds of immune-related lncRNAs to construct prognostic signatures to explore the mechanism, which provides a new way to study therapies for HCC.

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“…BUB1 promotes the occurrence and development of HCC by activating mTORC1-related proteins (33). PLK1 and CDK1 are important molecules in the abnormal cell cycle of HCC cells (34).…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics Profiling of Five Immune-Related lncRNAs for a Prognostic Model of Hepatocellular Carcinoma

Wei

Liu

et al. 2021

Front. Oncol.

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“…Dysregulation in BUB genes have been reported in many cancers [ 6 , 14 – 19 , 21 , 23 , 24 ]. However, the role that BUB factors play in sarcoma remains poorly understood.…”

Section: Discussionmentioning

confidence: 99%

Expression and prognosis analyses of BUB1, BUB1B and BUB3 in human sarcoma

Long

Tian

et al. 2021

Aging

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Budding Uninhibited By Benzimidazoles are a group of genes encoding proteins that play central roles in spindle checkpoint during mitosis. Improper mitosis may lead to aneuploidy which is found in many types of tumors. As a key mediator in mitosis, the dysregulated expression of BUBs has been proven to be highly associated with various malignancies, such as leukemia, gastric cancer, breast cancer, and liver cancer. However, bioinformatic analysis has not been applied to explore the role of the BUBs in sarcomas. Herein, we investigate the transcriptional and survival data of BUBs in patients with sarcomas using Oncomine, Gene Expression Profiling Interactive Analysis, Cancer Cell Line Encyclopedia, Kaplan-Meier Plotter, LinkedOmics, and the Database for Annotation, Visualization and Integrated Discovery. We found that the expression levels of BUB1, BUB1B and BUB3 were higher in sarcoma samples and cell lines than in normal controls. Survival analysis revealed that the higher expression levels of BUB1, BUB1B and BUB3 were associated with lower overall and disease-free survival in patients with sarcomas. This study implies that BUB1, BUB1B and BUB3 are potential treatment targets for patients with sarcomas and are new biomarkers for the prognosis of sarcomas.

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“…A recent study demonstrated that BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B) could promote the proliferation of prostate cancer [ 46 ]. BUB1B also promotes the progression of hepatocellular carcinoma by activating the mammalian target of the rapamycin complex 1 (mTORC1) signaling pathway [ 47 ]. However, the role and function of BUB1B in the crosstalk between G-SCs/P-SCs and OSCC have been poorly studied.…”

Section: Discussionmentioning

confidence: 99%

The Origin of Stroma Influences the Biological Characteristics of Oral Squamous Cell Carcinoma

Omori

Shan

Takabatake

et al. 2021

Cancers

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Normal stromal cells surrounding the tumor parenchyma, such as the extracellular matrix (ECM), normal fibroblasts, mesenchymal stromal cells, and osteoblasts, play a significant role in the progression of cancers. However, the role of gingival and periodontal ligament tissue-derived stromal cells in OSCC progression is unclear. In this study, the effect of G-SCs and P-SCs on the differentiation, proliferation, invasion, and migration of OSCC cells in vitro was examined by Giemsa staining, Immunofluorescence (IF), (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS), invasion, and migration assays. Furthermore, the effect of G-SCs and P-SCs on the differentiation, proliferation, and bone invasion by OSCC cells in vivo was examined by hematoxylin-eosin (HE) staining, immunohistochemistry (IHC), and tartrate-resistant acid phosphatase (TRAP) staining, respectively. Finally, microarray data and bioinformatics analyses identified potential genes that caused the different effects of G-SCs and P-SCs on OSCC progression. The results showed that both G-SCs and P-SCs inhibited the differentiation and promoted the proliferation, invasion, and migration of OSCC in vitro and in vivo. In addition, genes, including CDK1, BUB1B, TOP2A, DLGAP5, BUB1, and CCNB2, are probably involved in causing the different effects of G-SCs and P-SCs on OSCC progression. Therefore, as a potential regulatory mechanism, both G-SCs and P-SCs can promote OSCC progression.

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BUB1B promotes hepatocellular carcinoma progression via activation of the mTORC1 signaling pathway

Cited by 49 publications

References 37 publications

Bioinformatics Profiling of Five Immune-Related lncRNAs for a Prognostic Model of Hepatocellular Carcinoma

Bioinformatics Profiling of Five Immune-Related lncRNAs for a Prognostic Model of Hepatocellular Carcinoma

Expression and prognosis analyses of BUB1, BUB1B and BUB3 in human sarcoma

The Origin of Stroma Influences the Biological Characteristics of Oral Squamous Cell Carcinoma

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