Bromelain Inhibits Allergic Sensitization and Murine Asthma via Modulation of Dendritic Cells

Abstract

The incidence of atopic conditions has increased in industrialized countries. Persisting symptoms and concern for drug side-effects lead patients toward adjunctive treatments such as phytotherapy. Previously, we have shown that Bromelain (sBr), a mixture of cysteine proteases from pineapple, Ananas comosus, inhibits ovalbumin (OVA)-induced murine model of allergic airway disease (AAD). However, sBr's effect on development of AAD when treatment is administered throughout OVA-alum sensitization was unknown and is the aim of the present study. C57BL/6J mice were sensitized with OVA/alum and challenged with 7 days OVA aerosol. sBr 6 mg/kg/0.5 ml or PBS vehicle were administered throughout sensitization. Lung, bronchoalveolar lavage (BAL), spleen, and lymph nodes were processed for flow cytometry and OVA-specific IgE was determined via ELISA. sBr treatment throughout OVA-alum sensitization significantly reduced the development of AAD (BAL eosinophils and lymphocytes). OVA-specific IgE and OVA TET+ cells were decreased. sBr reduced CD11c+ dendritic cell subsets, and in vitro treatment of DCs significantly reduced CD44, a key receptor in both cell trafficking and activation. sBr was shown to reduce allergic sensitization and the generation of AAD upon antigen challenge. These results provide additional insight into sBr's anti-inflammatory and antiallergic properties and rationale for translation into the clinical arena.

Bromelain a Potential Bioactive Compound: A Comprehensive Overview from a Pharmacological Perspective

“…The total BAL leukocytes (eosinophils and lymphocytes) and cellular infiltrates are reduced by bromelain, thus relieving asthma symptoms [ 5 , 116 ]. Bromelain also prominently reduces BAL CD4 + , CD8 + , CD4 + T, and CD25 + T cells [ 5 , 117 ]; interleukins IL-4, IL-13, IL-12, IL-17, and IFN-α in the serum; and the ratio of CD4 + to CD8 + cells [ 5 , 115 , 116 , 118 , 119 ].…”

Does Bromelain-Cisplatin Combination Afford In-Vitro Synergistic Anticancer Effects on Human Prostatic Carcinoma Cell Line, PC3?

“…Further, the proteolytic properties of bromelain was advocated as to be the leading mechanism of its anticancer action [8]. Accordingly, bromelain displayed blocking cell adhesion, inhibiting cell migration and invasiveness on glioblastoma [8,11,[24][25][26][27].…”

“…Further, the proteolytic properties of bromelain was advocated as to be the leading mechanism of its anticancer action [ 8 ]. Accordingly, bromelain displayed blocking cell adhesion, inhibiting cell migration and invasiveness on glioblastoma [ 8 , 11 , 24 - 27 ]. The bromelain inhibitory effects on neoplastic cell proliferation (cytostatic or cytotoxic) were widely documented on various animal or human neoplasms such as leukemia, lymphoma, sarcoma, melanoma, lung carcinoma, gastric-intestinal carcinoma, gliolastoma, breast cancer, epidermoid carcinoma, melanoma and malignant mesothelioma [ 16 , 28 , 29 ].…”

A new bromelain-based enzyme for the release of Dupuytren’s contracture

“…The enzyme should be dissolved in a vehicle before injection. Bromelain, a clinically used natural pineapple product, has some reported anti-inflammatory and immunomodulatory activities; 22 , 23 these anti-inflammatory activites, alongside its proteolytic properties, may also help reduce the recurrence rate of DC.…”