Bridged Rebar Graphene functionalized aptasensor for pathogenic E. coli O78:K80:H11 detection

Kaur, Harmanjit; Shorie, Munish; Sharma, Manju; Ganguli, Ashok K.; Sabherwal, Priyanka

doi:10.1016/j.bios.2017.07.004

Cited by 70 publications

(26 citation statements)

References 28 publications

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“…The application of EIS in the ultrasensitive detection of bacterial pathogens E. coli and S. dysenteriae has been a proof in such cases. 133,134 Mechanical aptasensors…”

Section: Eis Based Aptasensorsmentioning

confidence: 99%

Nanomaterial based aptasensors for clinical and environmental diagnostic applications

Kaur

Shorie

2019

Nanoscale Adv.

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“…The application of EIS in the ultrasensitive detection of bacterial pathogens E. coli and S. dysenteriae has been a proof in such cases. 133,134 Mechanical aptasensors…”

Section: Eis Based Aptasensorsmentioning

confidence: 99%

Nanomaterial based aptasensors for clinical and environmental diagnostic applications

Kaur

Shorie

2019

Nanoscale Adv.

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“…Therefore, here we focus on the improvement of the working electrode in E. coli cell direct detection. As important electrode modification material, metal nanoparticles, graphene and single-walled carbon nanotubes (SWCNT) have been used in constructing electrochemical biosensors for improving the electron transfer and reducing the detection limitation [ 75 , 76 , 77 ]. Here, we present the latest advances for E. coli cell detection by using of electrochemical biosensors that combines metal nanoparticles, graphene and SWCNT.…”

Section: Aptamer-based Electrochemical Sensors For Pathogenic mentioning

confidence: 99%

Application of Aptamer-Based Biosensor for Rapid Detection of Pathogenic Escherichia coli

Zhao

Wang

Jia

et al. 2018

Sensors

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Pathogenic Escherichia coli (E. coli) widely exist in Nature and have always been a serious threat to the human health. Conventional colony forming units counting-based methods are quite time consuming and not fit for rapid detection for E. coli. Therefore, novel strategies for improving detection efficiency and sensitivity are in great demand. Aptamers have been widely used in various sensors due to their extremely high affinity and specificity. Successful applications of aptamers have been found in the rapid detection of pathogenic E. coli. Herein, we present the latest advances in screening of aptamers for E. coli, and review the preparation and application of aptamer-based biosensors in rapid detection of E. coli. Furthermore, the problems and new trends in these aptamer-based biosensors for rapid detection of pathogenic microorganism are also discussed.

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“…In order to develop a highly sensitive and specific immunosensor, a molecular recognition element (MRE) with a high affinity must firstly be immobilized onto the sensing interface. Specific antibodies/antigens [ 33 , 35 ] and aptamers [ 36 ] are two kinds of well-established MREs ( Figure 1 ). The antigen–antibody reaction is the basis of immunoanalysis in clinical laboratories, and modern immuno-labeling techniques using highly sensitive substances as labels have further broadened the scope of immunological techniques.…”

Section: Biosensors For Protein Immunoassaysmentioning

confidence: 99%

“…Nanomaterial-based EC immunosensors have shown extensive advantages, leading to a greater loading amount of MREs, a faster electron transfer speed, and a more convenient combination of signal amplification strategies [ 2 ]. For example, Au nanoparticles (NPs) [ 43 , 44 ], carbon nanotubes (CNTs) [ 21 ], and graphene [ 36 ] have been widely used to preserve the activity of biomolecules and to enhance the electron transfer between redox species, increasing sensitivity. In addition, modern EC immunosensors exhibit an excellent detection performance for whole blood tests in a fast and simple way [ 31 , 45 ], with remarkable sensitivity and selectivity, offering EC biosensors with a benefit for transition toward POC application [ 34 ].…”

Section: Biosensors For Protein Immunoassaysmentioning

confidence: 99%

“…Besides antibodies/antigens, aptamers have also been widely used as capture probes for the construction of immunosensors, named aptasensors, with good selectivity and stability. For example, based on bridged rebar graphene, a novel label-free aptasensor was built for pathogenic bacteria E. coli O78:K80:H11 detection using a screen-printed electrode and an impedance detection technique [ 36 ]. The specific anti- E. coli DNA aptamer with K d ~14 nM was immobilized on a bridged-rebar-graphene-nanostructured electrode and used as a capture probe, showing an LOD as low as ~10 cfu mL −1 .…”

Section: Biosensors For Protein Immunoassaysmentioning

confidence: 99%

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Construction and Potential Applications of Biosensors for Proteins in Clinical Laboratory Diagnosis

Jiang

2017

Sensors

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Biosensors for proteins have shown attractive advantages compared to traditional techniques in clinical laboratory diagnosis. In virtue of modern fabrication modes and detection techniques, various immunosensing platforms have been reported on basis of the specific recognition between antigen-antibody pairs. In addition to profit from the development of nanotechnology and molecular biology, diverse fabrication and signal amplification strategies have been designed for detection of protein antigens, which has led to great achievements in fast quantitative and simultaneous testing with extremely high sensitivity and specificity. Besides antigens, determination of antibodies also possesses great significance for clinical laboratory diagnosis. In this review, we will categorize recent immunosensors for proteins by different detection techniques. The basic conception of detection techniques, sensing mechanisms, and the relevant signal amplification strategies are introduced. Since antibodies and antigens have an equal position to each other in immunosensing, all biosensing strategies for antigens can be extended to antibodies under appropriate optimizations. Biosensors for antibodies are summarized, focusing on potential applications in clinical laboratory diagnosis, such as a series of biomarkers for infectious diseases and autoimmune diseases, and an evaluation of vaccine immunity. The excellent performances of these biosensors provide a prospective space for future antibody-detection-based disease serodiagnosis.

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Bridged Rebar Graphene functionalized aptasensor for pathogenic E. coli O78:K80:H11 detection

Cited by 70 publications

References 28 publications

Nanomaterial based aptasensors for clinical and environmental diagnostic applications

Nanomaterial based aptasensors for clinical and environmental diagnostic applications

Application of Aptamer-Based Biosensor for Rapid Detection of Pathogenic Escherichia coli

Construction and Potential Applications of Biosensors for Proteins in Clinical Laboratory Diagnosis

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