Breastfeeding predicts blood mitochondrial DNA content in adolescents

Cosemans, Charlotte; Nawrot, Tim S.; Janssen, Bram G.; Vriens, Annette; Smeets, Karen; Baeyens, Willy; Bruckers, Liesbeth; Hond, Elly Den; Loots, Ilse; Nelen, Vera; Larebeke, Nicolas Van; Schoeters, Greet; Martens, Dries S.; Plusquin, Michelle

doi:10.1038/s41598-019-57276-z

Cited by 5 publications

(3 citation statements)

References 80 publications

(76 reference statements)

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“…The percentage of persons below LOD for each metal is shown in the Supplementary Table S1. For >50% below LOD (Mn) also investigated as binary variables, coded 0 when below the LOD and 1 when above the LOD (22). To reduce the effect of urine dilution, the metal concentrations were corrected for urinary creatinine and expressed as micrograms per gram of creatinine.…”

Section: Exposure Assessmentmentioning

confidence: 99%

Sex-specific associations of urinary mixed-metal concentrations with femoral bone mineral density among older people: an NHANES (2017–2020) analysis

Li,

et al. 2024

Front. Public Health

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BackgroundHeavy metal exposure is an important cause of reduced bone mineral density (BMD). Epidemiological studies focusing on the effects of mixed heavy metal exposure on BMD in middle-aged and older people are scarce. In single-metal studies, men and women have shown distinct responses of BMD to environmental metal exposure. This study therefore aimed to elucidate the association between mixed heavy metal exposure and BMD and to investigate whether it is sex-specific.MethodsData from the 2017–2020 National Health and Nutrition Examination Survey were selected for this cross-sectional study. The study used three statistical methods, i.e., linear regression, Bayesian kernel machine regression (BKMR) modeling, and weighted quartiles (WQS) regression, to explore the association between the urinary concentrations of 11 metals (barium, cadmium, cobalt, cesium, manganese, molybdenum, lead, antimony, tin, thallium, and Tungsten), either individually or as a mixture, and total femoral BMD.ResultsA total of 1,031 participants were included in this study. Femoral BMD was found to be higher in men than women. A significant negative correlation between the urinary concentrations of the 10 metals and femoral BMD was found in the overall cohort. Further gender sub-stratified analyses showed that in men, urinary metal concentrations were negatively correlated with femoral BMD, with cobalt and barium playing a significant and non-linear role in this effect. In women, although urinary metal concentrations negatively modulated femoral BMD, none of the correlations was statistically significant. Antimony showed sex-specific differences in its effect.ConclusionThe urinary concentrations of 10 mixed heavy metals were negatively correlated with femoral BMD in middle-aged and older participants, and this effect showed gender differences. These findings emphasize the differing role of mixed metal exposure in the process of BMD reduction between the sexes but require further validation by prospective studies.

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Section: Exposure Assessmentmentioning

confidence: 99%

Sex-specific associations of urinary mixed-metal concentrations with femoral bone mineral density among older people: an NHANES (2017–2020) analysis

Li,

et al. 2024

Front. Public Health

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“…Cord blood mtDNA content was measured by determining the ratio of two mitochondrial gene copy numbers (MTF3212/R3319 and MT-ND1) to two single-copy nuclear control genes (RPLP0 and ACTB) using the 7900HT Fast Real-Time PCR System (Applied Biosystems). The full protocol is described elsewhere (23).…”

Section: Mitochondrial Dna Contentmentioning

confidence: 99%

Exploring Mitochondrial Heteroplasmy in Neonates: Implications for Growth Patterns in the First Years of Life

Cosemans,

Alfano,

Sleurs

et al. 2023

Preprint

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Background: Mitochondrial heteroplasmy reflects genetic diversity within individuals due to the presence of varying mitochondrial DNA (mtDNA) sequences, possibly affecting mitochondrial function and energy production in cells. Rapid growth during early childhood is a critical development with long-term implications for health and well-being. In this study, we investigated if cord blood mtDNA heteroplasmy is associated with rapid growth at six and 12 months and overweight in childhood at four to six years. Methods: This study included 200 mother-child pairs of the ENVIRONAGE birth cohort. Whole mitochondrial genome sequencing was performed to determine mtDNA heteroplasmy levels (in variant allele frequency; VAF) in cord blood. Rapid growth was defined for each child as the difference between WHO-SD scores of predicted weight at either six or 12 months and birth weight. Logistic regression models were used to determine the association of mitochondrial heteroplasmy with rapid growth and childhood overweight. Determinants of relevant cord blood mitochondrial heteroplasmies were identified using multiple linear regression models. Results: One % increase in VAF of cord blood MT-D-Loop16362T>C heteroplasmy was associated with rapid growth at six (OR=1.03; 95% CI: 1.01 to 1.05; p=0.001) and 12 months (OR=1.02; 95% CI: 1.00 to 1.03; p=0.02). Furthermore, this variant was associated with childhood overweight at four to six years (OR=1.01; 95% CI 1.00 to 1.02; p=0.05). Additionally, rapid growth at six (OR=3.00; 95% CI: 1.49 to 6.14; p=0.002) and 12 months (OR=4.05; 95% CI: 2.06 to 8.49; p<0.001) was also associated with childhood overweight at four to six years. Furthermore, we identified maternal age, pre-pregnancy BMI, maternal education, parity, and gestational age as determinants of cord blood MT-D-Loop16362T>C heteroplasmy. Conclusions: Our findings, based on mitochondrial DNA genotyping, offer insights into the molecular machinery leading to rapid growth in early life, potentially explaining a working mechanism of the development towards childhood overweight.

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“…In addition, on a molecular level, being breastfed during infancy was associated with blood mtDNA content in adolescence. More precisely, the longer the adolescent was postnatally breastfed, the higher the blood mtDNA content was during adolescence, suggesting a duration-dependent effect of breastfeeding ( 7 ).…”

Section: Introductionmentioning

confidence: 99%

Black carbon particles in human breast milk: assessing infant’s exposure

Cosemans,

Bongaerts,

Vanbrabant

et al. 2024

Front. Public Health

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Background/AimHuman breast milk is the recommended source of nutrition for infants due to its complex composition and numerous benefits, including a decline in infection rates in childhood and a lower risk of obesity. Hence, it is crucial that environmental pollutants in human breast milk are minimized. Exposure to black carbon (BC) particles has adverse effects on health; therefore, this pilot study investigates the presence of these particles in human breast milk.MethodsBC particles from ambient exposure were measured in eight human breast milk samples using a white light generation under femtosecond illumination. The carbonaceous nature of the particles was confirmed with BC fingerprinting. Ambient air pollution exposures (PM2.5, PM10, and NO2) were estimated using a spatial interpolation model based on the maternal residential address. Spearman rank correlation coefficients were obtained to assess the association between human breast milk’s BC load and ambient air pollution exposure.ResultsBC particles were found in all human breast milk samples. BC loads in human breast milk were strongly and positively correlated with recent (i.e., 1 week) maternal residential NO2 (r = 0.79; p = 0.02) exposure and medium-term (i.e., 1 month) PM2.5 (r = 0.83; p = 0.02) and PM10 (r = 0.93; p = 0.002) exposure.ConclusionFor the first time, we showed the presence of BC particles in human breast milk and found a robust association with ambient air pollution concentrations. Our findings present a pioneering insight into a novel pathway through which combustion-derived air pollution particles can permeate the delicate system of infants.

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Breastfeeding predicts blood mitochondrial DNA content in adolescents

Cited by 5 publications

References 80 publications

Sex-specific associations of urinary mixed-metal concentrations with femoral bone mineral density among older people: an NHANES (2017–2020) analysis

Sex-specific associations of urinary mixed-metal concentrations with femoral bone mineral density among older people: an NHANES (2017–2020) analysis

Exploring Mitochondrial Heteroplasmy in Neonates: Implications for Growth Patterns in the First Years of Life

Black carbon particles in human breast milk: assessing infant’s exposure

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