Brazilian red propolis effects on peritoneal macrophage activity: Nitric oxide, cell viability, pro-inflammatory cytokines and gene expression

Bueno‐Silva, Bruno; Kawamoto, Dione; Ando-Suguimoto, Ellen S.; Casarin, Renato Corrêa Viana; Alencar, Severino Matias de; Rosalen, Pedro Luiz; Mayer, Márcia Pinto Alves

doi:10.1016/j.jep.2017.06.015

Cited by 44 publications

(39 citation statements)

References 80 publications

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“…In this study, the expression of IL-1β was significantly decreased in the presence of 0.15 and 1.5 µg/ml of PEEP. In line with these results, 60 µg/ml of BRP indicated inhibitory effects on IL-1α, IL-1β, and IL-6 levels (Bueno-Silva et al, 2017). In contrast, Bachiega et al showed that macrophages incubattion with propolis, cinnamic, and coumaric acids were increased production of IL-1β at all concentrations (5, 10, 25, 50, and 100 µg/well), suggesting that the given effects were likely to be due to the synergistic action of its components, because coumaric acids could produce IL-1β higher than propolis alone.…”

Section: Effects Of Peep On Inflammatory Genes Expressionsupporting

confidence: 83%

“…In the study on EEP in Turkey, the inhibition of NO production with IC 50 value of 0.1 ± 0.1 μg/ml without causing any cytotoxic effect on iNOS was reported (Onbas et al, ). In another investigation, Brazilian red propolis (BRP 60 μg/ml) inhibited 65% of NO production without any effects on cell viability (Bueno‐Silva et al, ). Additionally, Paulino et al demonstrated decreased NO production in LPS‐stimulated RAW 264.7 cells at low concentrations (Paulino et al, ).…”

Section: Resultsmentioning

confidence: 98%

“…Among proinflammatory cytokines, IL-1β is known as a potent pro-inflammatory cytokine expressed in response to microbial stimuli (Bachiega, Orsatti, Pagliarone, & Sforcin, 2012;Dinarello, 2010;Turner et al, 2014). So, the inhibition of IL-1β led to the inhibition of a cascade that activated NF-κB, iNOS, and pro-inflammatory cytokines (Bueno-Silva et al, 2017) which indicated important roles of this cytokine in the inflammatory response (Bachiega et al, 2012). In this study, the expression of IL-1β was significantly decreased in the presence of 0.15 and 1.5 µg/ml of PEEP.…”

Section: Effects Of Peep On Inflammatory Genes Expressionmentioning

confidence: 99%

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Propolis attenuates lipopolysaccharide‐induced inflammatory responses through intracellular ROS and NO levels along with downregulation of IL‐1β and IL‐6 expressions in murine RAW 264.7 macrophages

et al. 2019

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Propolis had a wide spectrum of biological activities. In the current study, antioxidative and the immunomodulatory effects of the Polur ethanol extract of propolis (PEEP) in murine macrophage (RAW 264.7) cells were investigated. Bioactive composition of the PEEP was determined by HPLC analysis. Cells were treated with different concentrations of PEEP and LPS, then cell viability, NO levels, and expression of inflammatory factors were evaluated. HPLC analysis of PEEP indicated the presence of flavonoids and phenolic acid. The PEEP inhibited the proliferation of RAW 264.7 cells with IC50 15 ± 3.2 µg/ml. Reactive oxygen species (ROS) and NO production was significantly reduced by 0.15 µg/ml of PEEP. Additionally, expression of Cox‐2, IL‐1β and IL‐6 significantly decreased. The obtained results supported the PEEP anti‐inflammatory effects on RAW 264.7 cells may be applied via reducing ROS and NO production along with COX‐2, IL‐1β, and IL‐6 expression. Practical applications Propolis is a resinous substance produced by the honeybee that has been adopted as a form of traditional medicine since ancient times. The main compounds found in propolis are typically various and depend on the type of plants and climatic region. In this respect, a wide spectrum of biological activities for propolis has been identified including antioxidant, antimicrobial, anticarcinogenic, anti‐inflammatory, as well as antifungal properties. This extraordinary substance is rich in flavonoids and antioxidants. Therefore, it is now widely used in foods and drinks with the claim that it can maintain or improve human health.

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Section: Effects Of Peep On Inflammatory Genes Expressionsupporting

confidence: 83%

Section: Resultsmentioning

confidence: 98%

Section: Effects Of Peep On Inflammatory Genes Expressionmentioning

confidence: 99%

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Propolis attenuates lipopolysaccharide‐induced inflammatory responses through intracellular ROS and NO levels along with downregulation of IL‐1β and IL‐6 expressions in murine RAW 264.7 macrophages

et al. 2019

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“…This was due to the complex interactions between the various cytokines, including IL-10, IL-4, IL-13, and TNF. 28 The regulation of capping medicaments in hDPC migration may affect the healing of the remaining dental pulp and the formation of dentin bridges. In this experiment, propolis was found to inhibit cell migration to a certain degree, while MTA promoted cell migration significantly.…”

Section: Discussionmentioning

confidence: 99%

Effects of MTA and Brazilian propolis on the biological properties of dental pulp cells

2019

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The aim of this study was to evaluate the effect of mineral trioxide aggregate (MTA) and Brazilian propolis on the cell viability, mineralization, anti-inflammatory ability, and migration of human dental pulp cells (hDPCs). The cell viability was evaluated with CCK-8 kit after 1, 5, 7, and 9 days. The deposition of calcified matrix and the expression of osteogenesis-related genes were evaluated by Alizarin Red staining and real-time PCR after incubation in osteogenic medium for 21 days. The expression of inflammation-related genes in cells was determined after exposure to 1 μg/mL LPS for 3 h. Finally, the numbers of cells that migrated through the permeable membranes were compared during 15 h. Propolis and MTA significantly increased the viability of hDPCscompared to the control group on days 7 and 9. In the propolis group, significant enhancement of osteogenic potential and suppressed expression of IL-1β and IL-6 was observed after LPS exposure compared to the MTA and control groups. The number of migration cells in the propolis group was similar to that of the control group, while MTA significantly promoted cell migration. Propolis showed comparable cell viability to that of MTA and exhibited significantly higher anti-inflammatory and mineralization promotion effects on hDPCs.

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“…PGES gene expression is modulated by the NF‐κB transcription factor, an important signaling pathway on inflammation and cancer diseases because its activation leads to the PGES gene expression (Korbecki, Baranowska‐Bosiacka, Gutowska, & Chlubek, ). Previously, our group found that treatment with neovestitol decreases the NF‐κB transcription factor activation (Bueno‐Silva et al, ). The present results help to explain the mechanism of action of neovestitol in malignant cells, where this molecule reduces NF‐κB activation, leading to a decrease in the PGES gene expression and ultimately reducing cancer cell activity and promoting cell death.…”

Section: Resultsmentioning

confidence: 95%

Isoflavonoids from Brazilian red propolis down‐regulate the expression of cancer‐related target proteins: A pharmacogenomic analysis

Nani

Franchin

Lazarini

et al. 2018

Phytotherapy Research

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Vestitol and neovestitol are bioactive isoflavonoids isolated from Brazilian red propolis, a unique Apis melifera type of propolis botanically originated from Dalbergia ecastophyllum. Although these molecules have relevant biological effects, including anticancer and immunomodulatory activities, their mechanism(s) of action and the affected pathways remain largely unknown. Here, we carried out a pharmacogenomic analysis to investigate the effects of vestitol and neovestitol on the whole-genome expression in human tumor cells, particularly cancer-related target proteins. HeLa cells were exposed to the compounds at IC and genomic information of treated cells was analyzed using the Illumina transcriptome system and GeneGo MetaCore software. Our results showed that vestitol (IC = 214.7 μM) reduced the expression of genes enrolled with the alpha tubulin (fold -3.7), tubulin in microtubules (fold -3.7), and histone h3 (fold = -3.03), and that treatment with neovestitol (IC = 102.91 μM) downregulated prostaglandin E synthase gene (fold = -3.12), which are considered ideal targets for anticancer therapy. These data open avenues for the study of vestitol and neovestitol as potential promising candidates for anticancer therapy. Toxicological, non-clinical, and clinical validation of the findings presented herein is needed.

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Brazilian red propolis effects on peritoneal macrophage activity: Nitric oxide, cell viability, pro-inflammatory cytokines and gene expression

Cited by 44 publications

References 80 publications

Propolis attenuates lipopolysaccharide‐induced inflammatory responses through intracellular ROS and NO levels along with downregulation of IL‐1β and IL‐6 expressions in murine RAW 264.7 macrophages

Propolis attenuates lipopolysaccharide‐induced inflammatory responses through intracellular ROS and NO levels along with downregulation of IL‐1β and IL‐6 expressions in murine RAW 264.7 macrophages

Effects of MTA and Brazilian propolis on the biological properties of dental pulp cells

Isoflavonoids from Brazilian red propolis down‐regulate the expression of cancer‐related target proteins: A pharmacogenomic analysis

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