Effects of MTA and Brazilian propolis on the biological properties of dental pulp cells

Shi, Bingqing; Zhao, Yuming; Yuan, Xiaojing

doi:10.1590/1807-3107bor-2019.vol33.0117

Cited by 9 publications

(10 citation statements)

References 35 publications

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“…Peycheva [ 50 ] demonstrated that the expression of pro-inflammatory cytokines such as IL-1β, IL-6, TNF-α, IL-17A, IL-18, and INF-γ was significantly reduced in the gingival crevicular fluid isolated from adolescents with moderate gingivitis after receiving toothpaste containing propolis [ 50 ]. Similar observations on the effect of propolis in reducing the expression of pro-inflammatory cytokines were also made in studies investigating the effect of propolis on human periodontal ligament cells and human dental pulp cells and tissues [ 51 , 52 , 53 ]. In addition, propolis appeared to inhibit high glucose-induced NLRP3 inflammasome activation by downregulating the expression of NLRP3, caspase-1 and IL-1β mRNA and their associated protein levels [ 54 ].…”

Section: Resultssupporting

confidence: 71%

Recent Update on the Anti-Inflammatory Activities of Propolis

et al. 2022

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In recent years, research has demonstrated the efficacy propolis as a potential raw material for pharmaceuticals and nutraceuticals. There is limited report detailing the mechanisms of action of propolis and its bioactive compounds in relation to their anti-inflammatory properties. Thus, the aim of the present review is to examine the latest experimental evidence (2017–2022) regarding the anti-inflammatory properties of propolis. A systematic scoping review methodology was implemented. After applying the exclusion criteria, a total of 166 research publications were identified and retrieved from Scopus, Web of Science, and Pubmed. Several key themes related to the anti-inflammatory properties of propolis were subsequently identified, namely in relation to cancers, oral health, metabolic syndrome, organ toxicity and inflammation, immune system, wound healing, and pathogenic infections. Based on the latest experimental evidence, propolis is demonstrated to possess various mechanisms of action in modulating inflammation towards the regulatory balance and anti-inflammatory environment. In general, we summarize that propolis acts as an anti-inflammatory substance by inhibiting and downregulating TLR4, MyD88, IRAK4, TRIF, NLRP inflammasomes, NF-κB, and their associated pro-inflammatory cytokines such as IL-1β, IL-6, IFN-γ, and TNF-α. Propolis also reduces the migration of immune cells such as macrophages and neutrophils, possibly by downregulating the chemokines CXCL9 and CXCL10.

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Section: Resultssupporting

confidence: 71%

Recent Update on the Anti-Inflammatory Activities of Propolis

et al. 2022

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“…www.nature.com/scientificreports/ 96% purity, extracted by an enzyme digestion method from healthy unerupted human third molars were used. Transwell membranes with a pore diameter of 0.4 µm were used to mimic the absence of direct contact between the biomaterial and hDPSCs while allowing for soluble compounds from the biomaterials to reach the cells, similar to clinical conditions 17 . TCS-based cements are known to release Ca 2+ in varying concentration, depending on the cement composition.…”

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confidence: 99%

The calcium dynamics of human dental pulp stem cells stimulated with tricalcium silicate-based cements determine their differentiation and mineralization outcome

Rathinam

Govindarajan

Rajasekharan

et al. 2021

Sci Rep

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Calcium (Ca2+) signalling plays an indispensable role in dental pulp and dentin regeneration, but the Ca2+ responses of human dental pulp stem cells (hDPSCs) stimulated with tricalcium silicate-based (TCS-based) dental biomaterials remains largely unexplored. The objective of the present study was to identify and correlate extracellular Ca2+ concentration, intracellular Ca2+ dynamics, pH, cytotoxicity, gene expression and mineralization ability of human dental pulp stem cells (hDPSCs) stimulated with two different TCS-based biomaterials: Biodentine and ProRoot white MTA. The hDPSCs were exposed to the biomaterials, brought in contact with the overlaying medium, with subsequent measurements of extracellular Ca2+ and pH, and intracellular Ca2+ changes. Messenger RNA expression (BGLAP, TGF-β, MMP1 and BMP2), cytotoxicity (MTT and TUNEL) and mineralization potential (Alizarin red and Von Kossa staining) were then evaluated. Biodentine released significantly more Ca2+ in the α-MEM medium than ProRoot WMTA but this had no cytotoxic impact on hDPSCs. The larger Biodentine-linked Ca2+ release resulted in altered intracellular Ca2+ dynamics, which attained a higher maximum amplitude, faster rise time and increased area under the curve of the Ca2+ changes compared to ProRoot WMTA. Experiments with intracellular Ca2+ chelation, demonstrated that the biomaterial-triggered Ca2+ dynamics affected stem cell-related gene expression, cellular differentiation and mineralization potential. In conclusion, biomaterial-specific Ca2+ dynamics in hDPSCs determine differentiation and mineralization outcomes, with increased Ca2+ dynamics enhancing mineralization.

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“…[28,29] Therefore, the ideal pulp capping material must be biocompatible, nontoxic, bactericide, anti-inflammation, antioxidative, and inducer of regenerative procedure in the damaged pulp tissue. [30] The application of natural bioactive ingredients attracts lots of attention in the regenerative medicine field. CUR is an ancient natural active ingredient, which accelerates healing procedures in different damaged organs.…”

Section: Discussionmentioning

confidence: 99%

Synthesis, characterization, and evaluation of curcumin‐loaded endodontic reparative material

Alipour

Fadakar

Aghazadeh

et al. 2021

J Biochem & Molecular Tox

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Curcumin (CUR) is an ancient therapeutic agent with remarkable antimicrobial and antiinflammatory properties. The purpose of the current study was to synthesize and evaluate a curcumin-based reparative endodontic material to reduce infection and inflammation besides the induction of mineralization during the healing of the dentin-pulp complex.Poly-ɛ-caprolactone (PCL)/gelatin (Gel)/CUR scaffold was synthesized and assessed by scanning electron microscopy, Fourier transform infrared spectroscopy, and thermogravimetric analysis (TGA). Agar diffusion test was performed against E. coli, A. baumannii, P. aeruginosa, S. aureus, E. faecalis, and S. mutans. Moreover, proliferative, antioxidative, anti-inflammatory, and calcification properties of these scaffolds on human dental pulp stem cells (hDPSCs) were evaluated. The results showed that PCL/Gel/CUR scaffold had antibacterial effects. Also, these CUR-based scaffolds had significant inhibitory effects on the expression of tumor necrosis factor α and DCF from inflamed hDPSCs (p < 0.05).Moreover, the induction of mineralization in hDPSCs significantly increased after seeding on CUR-based scaffolds (p < 0.05). Based on these findings, the investigated CUR-loaded material was fabricated successfully and provided an appropriate structure for the attachment and proliferation of hDPSCs. It was found that these scaffolds had antimicrobial, antioxidant, and anti-inflammatory characteristics and could induce mineralization in hDPSCs, which is essential for healing and repairing the injured dentin-pulp complex.

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Effects of MTA and Brazilian propolis on the biological properties of dental pulp cells

Cited by 9 publications

References 35 publications

Recent Update on the Anti-Inflammatory Activities of Propolis

Recent Update on the Anti-Inflammatory Activities of Propolis

The calcium dynamics of human dental pulp stem cells stimulated with tricalcium silicate-based cements determine their differentiation and mineralization outcome

Synthesis, characterization, and evaluation of curcumin‐loaded endodontic reparative material

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